公开(公告)号：US20100270174A1

公开(公告)日：2010-10-28

申请号：US12161683

申请日：2006-01-20

Applicant: Xian Tong Chen ,  Han Hua Feng ,  Min Bin Yu ,  Guo Qiang Lo

Inventor： Xian Tong Chen ,  Han Hua Feng ,  Min Bin Yu ,  Guo Qiang Lo

IPC: G01N27/26 ,  G01N27/327

CPC classification number: C12Q1/6825 ,  B01L3/5027 ,  G01N27/4145 ,  G01N33/54313 ,  G01N33/5438 ,  G01N33/553 ,  G01N33/585 ,  C12Q2565/607

Abstract: A biosensor cell (10) and biosensor array comprising a plurality of biosensor cells (10), each biosensor cell (10) comprising a sensing zone. The first sensing electrode (24), a second sensing electrode (25) and the gap (27) separating the sensing electrodes (24,25) are arranged within the sensing zone. The first sensing electrode (24) is electrically insulated from the second sensing electrode (25) by means of the gap (27). Capture molecules (28) are immobilised in the sensing zone; and a field effect transistor (16) having a gate electrode (19), a source electrode (17) and a drain electrode (18); the first sensing electrode (24) being electrically connected to the gate electrode (19) of the field effect transistor (16); and the second sensing electrode (25) being electrically connectable to a gate voltage. The invention also provides a method of detecting a target molecule such as a biomolecule.

Abstract translation: 一种生物传感器单元（10）和包括多个生物传感器单元（10）的生物传感器阵列，每个生物传感器单元（10）包括感测区域。 第一感测电极（24），第二感测电极（25）和分离感测电极（24,25）的间隙（27）布置在感测区域内。 第一感测电极（24）借助间隙（27）与第二感测电极（25）电绝缘。 捕获分子（28）固定在传感区; 和具有栅电极（19），源电极（17）和漏电极（18）的场效应晶体管（16）。 所述第一感测电极（24）电连接到所述场效应晶体管（16）的栅电极（19）; 并且第二感测电极（25）可电连接到栅极电压。 本发明还提供了检测诸如生物分子的靶分子的方法。

公开(公告)号：US20100248284A1

公开(公告)日：2010-09-30

申请号：US12161681

申请日：2006-01-20

Applicant: Xian Tong Chen ,  Min Bin Yu ,  Han Hua Feng ,  Quo Qiang Lo

Inventor： Xian Tong Chen ,  Min Bin Yu ,  Han Hua Feng ,  Quo Qiang Lo

IPC: C12Q1/02 ,  G01N27/00 ,  H05K13/00

CPC classification number: G01N33/5438 ,  G01N33/48728 ,  Y10T29/49117

Abstract: A biosensor which comprises a substrate (101) having a buried electronic sensing element and a substrate surface (124) above the buried electronic sensing element; a structured top layer (125) covering the substrate surface (124), having a top surface above the substrate surface (124), and comprising at least one stimulation and/or sensing electrode (116) and a channel (121) for holding the biomolecule by means of suction through said channel (121) arranged between the top surface (125) and the substrate surface (124), the sensing electrode (116) being electrically coupled to the electronic sensing element; wherein the top surface (125) is provided for placing a biomolecule present in a sample solution thereupon, the sensing electrode (116) is provided for sensing electrical variations in and presence of the biomolecule.

Abstract translation: 一种生物传感器，包括具有掩埋电子感测元件的基底（101）和掩埋电子感测元件上方的基底表面（124） 覆盖衬底表面（124）的结构化顶层（125），其具有在衬底表面（124）上方的顶表面，并且包括至少一个刺激和/或感测电极（116）和用于保持 通过设置在顶表面（125）和衬底表面（124）之间的所述通道（121）的吸力的生物分子，所述感测电极（116）电耦合到所述电子感测元件; 其中提供顶表面（125）用于放置存在于样品溶液中的生物分子，感测电极（116）用于感测生物分子的电变化和存在。