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公开(公告)号：US20090054626A1
公开(公告)日：2009-02-26
申请号：US12282761
申请日：2007-03-13
IPC分类号： C07K14/435 , C07K1/14
CPC分类号： C07K14/43509 , A23J1/04 , A23L3/3526 , A23L3/37 , A23L7/109 , A23V2002/00 , A23V2250/543 , A23V2200/10
摘要： A crustacean-derived protein having antifreeze activity which under reduced conditions has a molecular weight, as measured by sodium dodecyl sulfate (SDS)—polyacrylamide gel electrophoresis, of about 37,000, about 16,000, and/or about 15,400; and the N-terminal amino acids are indicated by SEQ ID No. 1 or SEQ ID No. 2.Since this protein is excellent in comparison to the conventional antifreeze proteins from the standpoints of safety, activity, production rate, cost, and the like, use is possible in a wide range of fields, beginning with foods.
摘要翻译： 具有抗冻活性的甲壳动物衍生的蛋白质，其在降低条件下具有通过十二烷基硫酸钠（SDS） - 聚丙烯酰胺凝胶电泳测量的约37,000，约16,000和/或约15,400的分子量; 并且N-末端氨基酸由SEQ ID No.1或SEQ ID No.2指示。由于与安全性，活性，生产率，成本等的观点相比，该蛋白质与常规的防冻蛋白质相比是优异的 在广泛的领域中可以使用，从食物开始。
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公开(公告)号：US20120064196A1
公开(公告)日：2012-03-15
申请号：US13231605
申请日：2011-09-13
CPC分类号： A22C25/20 , A22C17/0026 , A23B4/06 , A23L17/10 , A23L17/70
摘要： The purpose of the present technology is to provide a frozen minced fish meat of high quality while effectively using a natural resource. During production of minced fish meat, after removal of the head, guts, and kidney tissue (kidneys), meat is harvested to produce a minced fish meat characterized in that gelation strength is not lowered. Specifically, this production method includes as essential steps a step of washing the fish body; a step of removing the head, guts, and kidney tissue; a washing step; and a meat harvesting step. Protease specific activity of the minced fish meat, as measured by the peptide quantitative analysis method using phenol test solution, is less than or equal to 0.001.
摘要翻译： 本技术的目的是提供一种高品质的冷冻切碎鱼肉，同时有效利用自然资源。 在生产切碎的鱼肉时，在去除头部，肠道和肾脏组织（肾脏）之后，收获肉以产生切碎的鱼肉，其特征在于凝胶强度不降低。 具体而言，该制造方法包括作为洗涤鱼体的步骤的必要步骤; 去除头，肠和肾组织的一个步骤; 洗涤步骤 和肉类收割步骤。 通过使用苯酚试验溶液的肽定量分析法测定的切碎的鱼肉的蛋白酶比活性小于或等于0.001。
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3.发明申请METHOD FOR PRODUCING SURIMI AND PASTE PRODUCT FROM FISH MEAT CONTAINING KIDNEY 审中-公开从含有肉桂的肉制造SURIMI和PASTE产品的方法公开(公告)号：US20110129587A1
公开(公告)日：2011-06-02
申请号：US12952976
申请日：2010-11-23
申请人： Mitsutoshi KUBOTA , Satoru CHIBA
发明人： Mitsutoshi KUBOTA , Satoru CHIBA
IPC分类号： A23L1/325
CPC分类号： A23L17/70
摘要： According to a method for producing a surimi by adding a protease inhibitor when producing a surimi from fish meat that contains kidney or tissue thereof, such as backbone meat, that is left over when using a filleting and deboning method to extract fillets from fish from which the head and internal organs have been removed, it is possible to produce a surimi and a paste product having stable quality from backbone meat obtained as a residue when producing fillets. The protease inhibitor is preferably a cysteine protease inhibitor.
摘要翻译： 根据通过添加蛋白酶抑制剂的方法，当从含有肾或其组织的鱼肉（例如骨骼肉）中制造鱼糜时，通过添加蛋白酶抑制剂，当使用切片和剔骨方法从鱼中提取鱼片时，残留 头部和内脏已经被去除，可以在生产鱼片时从作为残留物的骨肉生产具有质量稳定的鱼糜和糊状产品。 蛋白酶抑制剂优选为半胱氨酸蛋白酶抑制剂。
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公开(公告)号：US20090136649A1
公开(公告)日：2009-05-28
申请号：US12282789
申请日：2007-03-13
CPC分类号： C07K14/43509 , A23J1/04 , A23L3/3526 , A23L3/37 , A23L7/109 , A23V2002/00 , A23V2250/543 , A23V2200/10
摘要： A crustacean-derived protein, as measured by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, displays a non-reduced band of a molecular weight of about 200,000, displays reduced-form bands at about 86,000 and 90,000 molecular weights, and has N-terminal amino acids as indicated by SEQ ID No. 1 or SEQ ID No. 2.This protein is contained in large amounts in the crustacean shells that are largely discarded except for use for meal, chitin, and chitosan raw material. Thus large volume preparation, as well as inexpensive production, is possible. Moreover, application by addition to foods is easy since this is a food-derived protein. Since the protein of the present invention has ice nucleation activity and recrystallization inhibition activity for ice crystals, wide use is possible with the object of quality maintenance and the like of frozen food and the like.
摘要翻译： 通过十二烷基硫酸钠（SDS） - 聚丙烯酰胺凝胶电泳测得的甲壳糖衍生蛋白质显示分子量约为20万的非还原带，显示约86,000和90,000分子量的还原带，并具有N - 末端氨基酸，如SEQ ID No.1或SEQ ID No.2所示。该蛋白质大量被包含在除了用于膳食，几丁质和壳聚糖原料之外被大量丢弃的甲壳类壳中。 因此，大批量的制备以及廉价的生产是可能的。 此外，通过添加到食物中的应用是容易的，因为这是食物来源的蛋白质。 由于本发明的蛋白质具有冰晶核活性和冰晶的重结晶抑制活性，所以在冷冻食品等的质量保持等方面可以广泛使用。
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公开(公告)号：US20080220419A1
公开(公告)日：2008-09-11
申请号：US11665843
申请日：2005-11-02
IPC分类号： C12Q1/68 , C07H21/00 , C07H1/00 , C12N15/63 , C07K14/00 , C12N15/00 , C12N9/04 , C12N1/00 , C12P7/00
CPC分类号： C12N9/0077 , C07K2319/00
摘要： The present invention provides a method for preparing a hybrid gene. The method includes a step of amplifying a P450 gene fragment contained in a sample using primers designed on the basis of regions of a plurality of P450 in which amino acid sequences are highly conserved and a step of preparing the hybrid gene using the amplified fragments and a known P450 gene. The method includes no culturing step or a step of normalizing extracted DNAs and is useful in isolating a P450 gene from various microbial resources.The present invention further provides a fused cytochrome P450 monooxygenase containing a peptide which is linked to the C-terminus of a P450 protein with a linker portion disposed therebetween and which has the same function as that of a reductase domain contained in a cytochrome P450 monooxygenase originating from Rhodococcus sp. strain NCIMB 9784. This enables the construction of a high-efficiency electron transfer system useful for various P450 proteins and also enables the production of an active P450 monooxygenase.
摘要翻译： 本发明提供了一种制备杂交基因的方法。 该方法包括使用基于其中氨基酸序列高度保守的多个P450的区域设计的引物和使用扩增片段制备杂交基因的步骤来扩增样品中包含的P450基因片段的步骤，以及 已知的P450基因。 该方法不包括培养步骤或标准化提取的DNA的步骤，并且可用于从各种微生物资源中分离P450基因。 本发明还提供一种融合的细胞色素P450单加氧酶，其含有与P450蛋白的C末端连接的肽，其中连接部位置于其间，并且具有与源于细胞色素P450单加氧酶的还原酶结构域相同的功能 来自红球菌属 应用NCIMB 9784.这使得能够构建可用于各种P450蛋白质的高效电子转移系统，并且还能够生产活性P450单加氧酶。
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公开(公告)号：US20140017370A1
公开(公告)日：2014-01-16
申请号：US14007542
申请日：2012-03-21
申请人： Takayuki Ishida , Mitsutoshi Kubota
发明人： Takayuki Ishida , Mitsutoshi Kubota
IPC分类号： A23B4/20
摘要： Problem: The problem of the present invention is to provide a frozen unwashed minced fish meat that has high versatility similar to that of frozen surimi and that is capable of frozen storage. In particular, the problem of the present invention is to prevent the freezing-denaturation of frozen unwashed minced fish meat of a fish of the order Gadiformes that is widely used for frozen surimi.Means to Solve the Problem: A frozen uncooked fish meat product in which an alcohol having from 2 to 5 carbons and capable of being used in foodstuffs is added. Preferably, to the frozen uncooked fish meat product, sugars or sugar alcohols and/or salts are further added. A method for preventing freezing-denaturation of uncooked fish meat, the method comprising suppressing freezing-denaturation due to trimethylamine-N-oxide degradation by adding an alcohol having from 2 to 5 carbons that is capable of being used in foodstuffs to the uncooked fish meat.
摘要翻译： 问题：本发明的问题是提供一种具有与冷冻鱼糜类似的能够冷冻保存的高通用性的冷冻未切碎的切碎的鱼肉。 特别地，本发明的问题在于防止冷冻鱼肉的冷冻未变质的鱼肉的冷冻变性。 解决问题的手段：加入一种冷冻未煮熟的鱼肉制品，其中加入2至5个碳的醇并且能够用于食品中。 优选地，对于冷冻未煮熟的鱼肉制品，进一步加入糖或糖醇和/或盐。 一种防止未经烹煮的鱼肉冷冻变性的方法，所述方法包括通过加入能够用于食品中的2至5个碳原子的醇对未煮熟的鱼肉加以抑制由于三甲胺-N-氧化物降解而导致的冷冻变性 。
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