公开(公告)号：US08202736B2

公开(公告)日：2012-06-19

申请号：US12379704

申请日：2009-02-26

申请人： Noha Ahmed Mousa ,  Mais J. Jebrail ,  Mohamed Omar Abdelgawad ,  Aaron R. Wheeler ,  Robert Fredric Joseph Casper

发明人： Noha Ahmed Mousa ,  Mais J. Jebrail ,  Mohamed Omar Abdelgawad ,  Aaron R. Wheeler ,  Robert Fredric Joseph Casper

IPC分类号： G01N33/536

CPC分类号： B01L3/502761 ,  B01L3/502753 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0415 ,  G01N1/4055 ,  G01N33/74 ,  G01N2001/4061

摘要： A method of extracting hormones from a biological sample using digital microfluidic arrays is provided. Biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in an extraction step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with excellent precision and recovery.

摘要翻译： 提供了一种使用数字微流体阵列从生物样品中提取激素的方法。 将含有激素的生物样品在数字微流体阵列上干燥，通过溶解溶剂裂解，干燥，随后溶解在极性溶剂中，并在萃取步骤中进一步纯化，其中液滴通过一定体积的非极性溶剂输送。 本文公开的方法提供了自动化样品制备方法的显着优点，其能够以优异的精度和恢复从低样品体积中提取激素。

公开(公告)号：US09851365B2

公开(公告)日：2017-12-26

申请号：US13203701

申请日：2010-02-26

申请人： Noha Ahmed Mousa ,  Mais J. Jebrail ,  Mohamed Omar Abdelgawad ,  Aaron R. Wheeler ,  Robert Fredric Joseph Casper

发明人： Noha Ahmed Mousa ,  Mais J. Jebrail ,  Mohamed Omar Abdelgawad ,  Aaron R. Wheeler ,  Robert Fredric Joseph Casper

IPC分类号： G01N33/536 ,  G01N33/74 ,  B01L3/00 ,  G01N1/40

CPC分类号： G01N33/74 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0415 ,  G01N1/4055 ,  G01N2001/4061

摘要： Methods and devices for liquid-liquid extraction using digital microfluidic arrays are provided. A polar droplet is transported to a separation region containing a substantially non-polar solvent, where non-polar impurities may be extracted from the polar droplet while maintaining a distinct phase separation. In a preferred embodiment, biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in a separation step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with high precision and recovery.

公开(公告)号：US20120085645A1

公开(公告)日：2012-04-12

申请号：US13203701

申请日：2010-02-26

申请人： Noha Ahmed Mousa ,  Mais J. Jebrail ,  Mohamed Omar Abdelgawad ,  Aaron R. Wheeler ,  Robert Fredric Joseph Casper

发明人： Noha Ahmed Mousa ,  Mais J. Jebrail ,  Mohamed Omar Abdelgawad ,  Aaron R. Wheeler ,  Robert Fredric Joseph Casper

IPC分类号： G01N33/53 ,  G01N33/74 ,  B81B1/00 ,  G01N27/447

CPC分类号： G01N33/74 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0415 ,  G01N1/4055 ,  G01N2001/4061

摘要： Methods and devices for liquid-liquid extraction using digital microfluidic arrays are provided. A polar droplet is transported to a separation region containing a substantially non-polar solvent, where non-polar impurities may be extracted from the polar droplet while maintaining a distinct phase separation. In a preferred embodiment, biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in a separation step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with high precision and recovery.

摘要翻译： 提供了使用数字微流体阵列进行液 - 液萃取的方法和装置。 将极性液滴输送到包含基本上非极性溶剂的分离区域，其中非极性杂质可以从极性液滴中提取，同时保持不同的相分离。 在优选的实施方案中，含有激素的生物样品在数字微流体阵列上干燥，通过裂解溶剂裂解，干燥，随后溶解在极性溶剂中，并在分离步骤中进一步纯化，其中液滴通过一定量的非 - 极性溶剂。 本文公开的方法提供了能够以高精度和恢复从低样品体积提取激素的自动化样品制备方法的显着优点。

公开(公告)号：US20060212297A1

公开(公告)日：2006-09-21

申请号：US11206621

申请日：2005-08-18

申请人： Upendra Chaudhari ,  Mohamed Omar ,  Ganesh Ramaswamy

发明人： Upendra Chaudhari ,  Mohamed Omar ,  Ganesh Ramaswamy

IPC分类号： G10L11/00

CPC分类号： G10L25/48

摘要： A system, method and computer program product for performing blind change detection audio segmentation that combines hypothesized boundaries from several segmentation algorithms to achieve the final segmentation of the audio stream. Automatic segmentation of the audio streams according to the system and method of the invention may be used for many applications like speech recognition, speaker recognition, audio data mining, online audio indexing, and information retrieval systems, where the actual boundaries of the audio segments are required.

摘要翻译： 一种用于执行盲变检测音频分段的系统，方法和计算机程序产品，其组合来自若干分段算法的假设边界以实现音频流的最终分段。 根据本发明的系统和方法的音频流的自动分割可以用于诸如语音识别，说话人识别，音频数据挖掘，在线音频索引和信息检索系统之类的许多应用，其中音频段的实际边界是 需要。

公开(公告)号：US20100213074A1

公开(公告)日：2010-08-26

申请号：US12379704

申请日：2009-02-26

申请人： Noha Ahmed Mousa ,  Mais J. Jebrail ,  Mohamed Omar Abdelgawad ,  Aaron R. Wheeler ,  Robert Fredric Joseph Casper

发明人： Noha Ahmed Mousa ,  Mais J. Jebrail ,  Mohamed Omar Abdelgawad ,  Aaron R. Wheeler ,  Robert Fredric Joseph Casper

IPC分类号： G01N27/26 ,  C25B3/00 ,  G01N33/74

CPC分类号： B01L3/502761 ,  B01L3/502753 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0415 ,  G01N1/4055 ,  G01N33/74 ,  G01N2001/4061

摘要： A method of extracting hormones from a biological sample using digital microfluidic arrays is provided. Biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in an extraction step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with excellent precision and recovery.

摘要翻译： 提供了一种使用数字微流体阵列从生物样品中提取激素的方法。 将含有激素的生物样品在数字微流体阵列上干燥，通过溶解溶剂裂解，干燥，随后溶解在极性溶剂中，并在萃取步骤中进一步纯化，其中液滴通过一定体积的非极性溶剂输送。 本文公开的方法提供了自动化样品制备方法的显着优点，其能够以优异的精度和恢复从低样品体积中提取激素。

公开(公告)号：US20180120335A1

公开(公告)日：2018-05-03

申请号：US15853515

申请日：2017-12-22

申请人： Noha Ahmed MOUSA ,  Mais J. JEBRAIL ,  Mohamed Omar ABDELGAWAD ,  Aaron R. WHEELER ,  Robert Fredric Joseph CASPER

发明人： Noha Ahmed MOUSA ,  Mais J. JEBRAIL ,  Mohamed Omar ABDELGAWAD ,  Aaron R. WHEELER ,  Robert Fredric Joseph CASPER

IPC分类号： G01N33/74 ,  G01N1/40 ,  B01L3/00

CPC分类号： G01N33/74 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0415 ,  G01N1/4055 ,  G01N2001/4061

摘要： Methods and devices for liquid-liquid extraction using digital microfluidic arrays are provided. A polar droplet is transported to a separation region containing a substantially non-polar solvent, where non-polar impurities may be extracted from the polar droplet while maintaining a distinct phase separation. In a preferred embodiment, biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in a separation step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with high precision and recovery.

公开(公告)号：US06372001B1

公开(公告)日：2002-04-16

申请号：US09509625

申请日：2000-03-30

申请人： Mohamed Omar ,  Heinz-Werner Schankweiler ,  Robert M. Burgess

发明人： Mohamed Omar ,  Heinz-Werner Schankweiler ,  Robert M. Burgess

IPC分类号： B24D306

CPC分类号： B24D11/02 ,  B24D3/002 ,  B24D3/06 ,  B24D11/005 ,  B24D18/0018

摘要： An abrasive article comprising an abrasive fabric comprising a fabric having discrete areas of electro-deposited metal extending on or therethrough and having abrasive material embedded in the metal, a layer of adhesive, and a backing substrate, the abrasive fabric being bonded to the backing substrate by the layer of adhesive, characterized in that either (i) the surface of the abrasive fabric bonded to the backing has been roughened and/or (ii) the layer of adhesive comprises an epoxy-acrylate thermosetting adhesive.

摘要翻译： 一种磨料制品，包括研磨织物，所述研磨织物包括具有分散的电沉积金属区域的织物，所述织物在其上延伸或贯穿并且具有嵌入金属中的研磨材料，粘合剂层和背衬基底，所述研磨织物结合到背衬基底 通过粘合剂层，其特征在于（i）结合到背衬的研磨织物的表面已被粗糙化和/或（ii）粘合剂层包含环氧丙烯酸酯热固性粘合剂。