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    Multi-cistronic vectors for gene transfer protocols
    1.
    发明申请
    Multi-cistronic vectors for gene transfer protocols 审中-公开
    用于基因转移方案的多顺反子载体

    公开(公告)号：US20060263882A1

    公开(公告)日：2006-11-23

    申请号：US10490530

    申请日：2002-10-10

    申请人： Vito Fazio ,  Monica Rinaldi ,  Laura Sonzogni ,  Giancarlo Tonon ,  Gaetano Orsini

    发明人： Vito Fazio ,  Monica Rinaldi ,  Laura Sonzogni ,  Giancarlo Tonon ,  Gaetano Orsini

    IPC分类号： C12N15/86 ,  C12N15/09 ,  C12N5/06 ,  A61K48/00

    CPC分类号： C12N15/85 ,  A61K48/00 ,  A61K2039/53 ,  C12N2830/42 ,  C12N2840/203 ,  C12N2840/206

    摘要： The subject of the present invention is the construction of multicistronic eukaryotic plasmid expression vectors in which it is possible to express from two to four genes simultaneously and which are characterized by differently regulated bicistronic transcription units. The distinctive characteristic of these vectors is the presence of a CAP-independent translation initiation mechanism which is based on the ability of an IRES (internal ribosomal entry site) sequence to translate two proteins under the control of a single promoter. This family of multicistronic vectors can advantageously be used in various biotechnological applications in whcih the simultaneous expression of two or more genes is necessary, such as gene transfer protocols, DNA-immunization, or for the expression of different molecules in the same cell.

    摘要翻译： 本发明的主题是多顺反子真核质粒表达载体的构建，其中可以同时从两个到四个基因表达，其特征在于不同程度的双顺反子转录单位。 这些载体的独特特征是存在CAP独立的翻译起始机制，其基于IRES（内部核糖体进入位点）序列在单个启动子的控制下翻译两种蛋白质的能力。 这种多顺反子载体家族可有利地用于各种生物技术应用中，同时表达两种或多种基因是必需的，例如基因转移方案，DNA免疫或用于在同一细胞中表达不同分子。