公开(公告)号：US6107543A

公开(公告)日：2000-08-22

申请号：US348769

申请日：1994-12-02

Applicant: Michelle M. Sims ,  Neal L. First

Inventor： Michelle M. Sims ,  Neal L. First

IPC: C12N5/0735 ,  C12N15/873 ,  C12N15/09 ,  C12N5/00 ,  C12N15/00 ,  C12N15/63

CPC classification number: C12N15/873 ,  C12N5/0606 ,  C12N2500/14 ,  C12N2500/25 ,  C12N2500/90

Abstract: The use of totipotent embryonic stem cells to provide substantially identical cells for embryo cloning techniques is described. The method includes the culture of loose suspensions of inner cell mass cells of bovine animals to retrieve large populations of stem cells. The invention also describes the use of stem cells in various genetic manipulation techniques.

Abstract translation: 描述了使用全能胚胎干细胞提供基本上相同的胚胎克隆技术的细胞。 该方法包括培养牛动物的内细胞团细胞的松散悬浮液以获取大量的干细胞。 本发明还描述了干细胞在各种遗传操作技术中的应用。

公开(公告)号：US5213979A

公开(公告)日：1993-05-25

申请号：US139887

申请日：1987-12-30

Applicant: Neal L. First ,  Willard Eyestone

Inventor： Neal L. First ,  Willard Eyestone

IPC: A01K67/02 ,  A61K35/54 ,  C12N5/02 ,  C12N5/073 ,  C12R1/91

CPC classification number: C12N5/0604 ,  C12N2502/243

Abstract: The disclosure herein describes a bovine embryo culture medium which includes epithelial cells from the oviduct of a donor animal. Additionally, a process for maturing immature bovine oocytes in an in vitro bovine culture medium containing epithelial cells from a donor oviduct is described. As an alternative to co-culture of the embryos with the epithelial cells, it is described how a culture medium can be conditioned for the culture of bovine embryos by the culture of epithelial cells therein which are later removed.

Abstract translation: 本文的公开内容描述了一种牛胚培养基，其包括来自供体动物的输卵管的上皮细胞。 另外，描述了在含有来自供体输卵管的上皮细胞的体外牛培养基中成熟未成熟牛卵母细胞的方法。 作为胚胎与上皮细胞共培养的替代方案，描述了培养基如何通过其中去除的上皮细胞的培养来调节牛胚胎的培养。

公开(公告)号：US4994384A

公开(公告)日：1991-02-19

申请号：US113791

申请日：1987-10-27

Applicant: Randall S. Prather ,  Frank Barnes ,  James Robl ,  Neal L. First ,  Vincent F. Simmon

Inventor： Randall S. Prather ,  Frank Barnes ,  James Robl ,  Neal L. First ,  Vincent F. Simmon

IPC: A01K67/02 ,  A01K67/027 ,  C12N15/06 ,  C12N15/877

CPC classification number: C12N15/8771

Abstract: A method of multiplying bovine embryos by transfer of nuclei is described. The method includes enucleating a recipient oocyte, transferring the donor embryo nucleus to the enucleated recipient oocyte, and fusing the donor nucleus to the recipient oocyte by dielectrophoresis. The method is intended to allow the generation of multiplied genetically identical cattle.

Abstract translation: 描述了通过转移核来增加牛胚胎的方法。 该方法包括将受体卵母细胞去核，将供体胚胎核转移到去核的受体卵母细胞，并通过介导电泳将供体核融合到受体卵母细胞。 该方法旨在产生繁殖的遗传上相同的牛。