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    MODIFIED OIL ENCAPSULATING PROTEINS AND USES THEREOF
    4.
    发明申请
    MODIFIED OIL ENCAPSULATING PROTEINS AND USES THEREOF 有权
    改性油包封蛋白及其用途

    公开(公告)号：US20120278951A1

    公开(公告)日：2012-11-01

    申请号：US13460464

    申请日：2012-04-30

    申请人： Nicholas John Roberts ,  Richard William Scott ,  Somrutai Winichayakul ,  Marissa Roldan

    发明人： Nicholas John Roberts ,  Richard William Scott ,  Somrutai Winichayakul ,  Marissa Roldan

    IPC分类号： C07K14/415 ,  C12N15/82 ,  C12P7/64 ,  A01H5/00 ,  C07K19/00 ,  C12P21/00 ,  C12N15/29 ,  C12N5/10

    CPC分类号： C07K14/415 ,  C07K2319/00 ,  C07K2319/21 ,  C12N15/8243 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8257 ,  C12N15/8261 ,  C12N15/8269 ,  C12N15/8271 ,  C12N15/8273 ,  C12P7/6463 ,  Y02A40/146 ,  Y02P60/247

    摘要： The invention provides modified oleosins, including at least one artificially introduced cysteine, and methods and compositions for producing the modified oleosins. Also provided are polynucleotides encoding the modified oleosins, constructs and host cells comprising the polynucleotides, methods for producing oil bodies comprising the modified oleosins, in vivo and in vitro, and methods for producing oil in host cells and plants. The invention also provides methods for increasing the rate of CO2 assimilation in photosynthetic cells and plants, and involves reducing or preventing lipid recycling, and/or expressing modified oleosins with artificially introduced cysteine residues in the photosynthetic cells and plants. Also provided are methods for increasing oil production in plants, via expression of modified oleosins in the non-photosynthetic tissues/organs of plants. The method also optionally includes the step of extrating the oil from the non-photosynthetic tissues/organs of the plant, or processing the oil rich non-photosynthetic tissues/organs into animal or biofuel feedstocks.

    摘要翻译： 本发明提供了修饰的油质蛋白，包括至少一个人造引入的半胱氨酸，以及用于生产改性油质蛋白的方法和组合物。 还提供了编码经修饰的油质蛋白，构建体和包含多核苷酸的宿主细胞的多核苷酸，在体内和体外用于生产包含改性油质蛋白的油体的方法，以及在宿主细胞和植物中生产油的方法。 本发明还提供了增加光合细胞和植物中二氧化碳同化速率的方法，并且涉及减少或预防脂质循环，和/或在光合细胞和植物中人造引入的半胱氨酸残基表达修饰的油质蛋白。 还提供了通过在非光合组织/植物器官中表达改性油质蛋白来增加植物中的油生产的方法。 该方法还任选地包括从植物的非光合组织/器官中抽出油，或将富含油的非光合组织/器官加工成动物或生物燃料原料的步骤。

    Modified oil encapsulating proteins and uses thereof
    5.
    发明授权
    Modified oil encapsulating proteins and uses thereof 有权
    改性油包封蛋白及其用途

    公开(公告)号：US08987551B2

    公开(公告)日：2015-03-24

    申请号：US13460464

    申请日：2012-04-30

    申请人： Nicholas John Roberts ,  Richard William Scott ,  Somrutai Winichayakul ,  Marissa Roldan

    发明人： Nicholas John Roberts ,  Richard William Scott ,  Somrutai Winichayakul ,  Marissa Roldan

    IPC分类号： A01H1/00 ,  A01H5/10 ,  C12N15/82 ,  C12N5/04 ,  C07H21/04 ,  A61K36/00 ,  C07K14/415 ,  C12P7/64

    CPC分类号： C07K14/415 ,  C07K2319/00 ,  C07K2319/21 ,  C12N15/8243 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8257 ,  C12N15/8261 ,  C12N15/8269 ,  C12N15/8271 ,  C12N15/8273 ,  C12P7/6463 ,  Y02A40/146 ,  Y02P60/247

    摘要： The invention provides modified oleosins, including at least one artificially introduced cysteine, and methods and compositions for producing the modified oleosins. Also provided are polynucleotides encoding the modified oleosins, constructs and host cells comprising the polynucleotides, methods for producing oil bodies comprising the modified oleosins, in vivo and in vitro, and methods for producing oil in host cells and plants. The invention also provides methods for increasing the rate of CO2 assimilation in photosynthetic cells and plants, and involves reducing or preventing lipid recycling, and/or expressing modified oleosins with artificially introduced cysteine residues in the photosynthetic cells and plants. Also provided are methods for increasing oil production in plants, via expression of modified oleosins in the non-photosynthetic tissues/organs of plants. The method also optionally includes the step of extracting the oil from the non-photosynthetic tissues/organs of the plant, or processing the oil rich non-photosynthetic tissues/organs into animal or biofuel feedstocks.

    摘要翻译： 本发明提供了修饰的油质蛋白，包括至少一个人造引入的半胱氨酸，以及用于生产改性油质蛋白的方法和组合物。 还提供了编码经修饰的油质蛋白，构建体和包含多核苷酸的宿主细胞的多核苷酸，在体内和体外用于生产包含改性油质蛋白的油体的方法，以及在宿主细胞和植物中生产油的方法。 本发明还提供了增加光合细胞和植物中二氧化碳同化速率的方法，并且涉及减少或预防脂质循环，和/或在光合细胞和植物中人造引入的半胱氨酸残基表达修饰的油质蛋白。 还提供了通过在非光合组织/植物器官中表达改性油质蛋白来增加植物中的油生产的方法。 该方法还任选地包括从植物的非光合组织/器官中提取油或将富含油的非光合组织/器官加工成动物或生物燃料原料的步骤。