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    Target-Dependent Nucleic Acid Adapter
    4.
    发明申请
    Target-Dependent Nucleic Acid Adapter 有权
    靶向依赖型核酸适配器

    公开(公告)号：US20080268516A1

    公开(公告)日：2008-10-30

    申请号：US11631689

    申请日：2005-07-06

    Applicant: Jean-Pierre Perreault ,  Lucien Junior Bergeron

    Inventor： Jean-Pierre Perreault ,  Lucien Junior Bergeron

    IPC: C12N9/99 ,  C07H21/02 ,  C12N9/24

    CPC classification number: C12N15/1131 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/123 ,  C12N2310/3519 ,  C12N2320/50

    Abstract: In accordance with the present invention, there is provided a nucleic acid target-dependent adapter linked to a nucleic acid sequence. The adapter comprises linked together a biosensor having a specific sequence complementary to a target sequence of a substrate, the biosensor improving the specificity of the nucleic acid sequence for the substrate, and a blocker stem sequence complementary to a portion of the nucleic acid sequence. In absence of the target sequence of the substrate, the blocker stem sequence forms an intramolecular stem with the nucleic acid sequence linked thereto, preventing exposition of the sequence of the nucleic acid sequence, thus locking the nucleic acid sequence so linked to the adapter in an inactive conformation, and in presence of the target sequence, the blocker stem sequence dissociating from the nucleic acid sequence, thus exposing the nucleic acid sequence linked to the adapter in an active conformation.

    Abstract translation: 根据本发明，提供了与核酸序列连接的核酸靶依赖性衔接子。 适配器包括将具有与底物的靶序列互补的特定序列的生物传感器连接在一起，生物传感器提高了底物的核酸序列的特异性，以及与核酸序列的一部分互补的阻断茎序列。 在不存在底物的靶序列的情况下，阻断剂茎序列形成与其连接的核酸序列的分子内茎，防止核酸序列的序列的显露，从而锁定与适配器连接的核酸序列 在靶序列存在下，阻断茎干序列与核酸序列解离，从而将与衔接子连接的核酸序列以活性构象暴露。

    Log positioning and conveying apparatus
    5.
    发明授权
    Log positioning and conveying apparatus 失效
    日志定位和传送设备

    公开(公告)号：US06896019B2

    公开(公告)日：2005-05-24

    申请号：US10426868

    申请日：2003-05-01

    Applicant: René Achard ,  Robert Gauthier ,  Jean-Pierre Perreault ,  Eddy Ste-Croix

    Inventor： René Achard ,  Robert Gauthier ,  Jean-Pierre Perreault ,  Eddy Ste-Croix

    IPC: B23Q1/62 ,  B23Q7/05 ,  B23Q7/14 ,  B27B31/00 ,  B27B31/06 ,  B27F15/013 ,  B65G37/00 ,  B27F31/00

    CPC classification number: B23Q1/621 ,  B23Q7/055 ,  B23Q7/14 ,  B27B31/003 ,  B27B31/06

    Abstract: The log positioning and conveying apparatus is used for conveying a log from an upstream end to a downstream end, and for positioning a log according to a predetermined downstream position. The apparatus comprises an open frame and a number of powered conveying rollers arranged therein in a row. These rollers, in addition to being rotatable, are displaceable in two directions relative to the open frame, namely, in a vertical direction for correcting the position of the log vertically relative to the open frame, and in a horizontal direction transversal to the longitudinal axis of the conveying apparatus, for correcting the position of the log transversely relative to the longitudinal axis.

    Abstract translation: 原木定位和输送装置用于将原木从上游端输送到下游端，并根据预定的下游位置定位原木。 该装置包括一排开放框架和多个排列在其中的动力输送辊。 除了可旋转之外，这些辊子可以相对于开放框架在两个方向上移位，即在垂直方向上可以相对于打开的框架竖直地校正木材的位置，并且在水平方向上横向于纵向轴线 的传送装置，用于相对于纵向轴线横向地校正对数的位置。

    Molecular markers for the diagnosis of human diseases including Crohn's disease
    6.
    发明授权
    Molecular markers for the diagnosis of human diseases including Crohn's disease 失效
    用于诊断人类疾病包括克罗恩病的分子标记

    公开(公告)号：US6150096A

    公开(公告)日：2000-11-21

    申请号：US938783

    申请日：1997-09-26

    Applicant: Jean-Pierre Perreault ,  Daniel LaFontaine ,  Stephane Mercure

    Inventor： Jean-Pierre Perreault ,  Daniel LaFontaine ,  Stephane Mercure

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/6883 ,  C12Q2600/158

    Abstract: The present invention relates to an arbitrarily primed PCR-based method to identify genetic marker associated with a pathology, which comprises the steps of: a) collecting heterogeneous nucleic acid samples from diseased and healthy tissues; b) determining quantities of the nucleic acid pools by specific amplification of a fragment of glyceraldehyde-phosphate dehydrogenase (GAPDH) mRNA; c) amplifying nucleic acid pools of step b) using non-specific sense and antisense primers to obtain clear patterns of nucleic acid sequences; and d) subjecting amplified nucleic acid sequences to gel electrophoresis to obtain fingerprints of both diseased and healthy, wherein bands predominantly associated with diseased tissues are markers for the pathology. The present invention also relates to a RNA expression marker for the diagnosis of Crohn's disease, which comprises a 3.1 kb RNA sequence, and uses thereof.

    Abstract translation: 本发明涉及一种用于鉴定与病理相关的遗传标记的任意引发的基于PCR的方法，其包括以下步骤：a）从患病和健康组织收集异质核酸样品; b）通过甘油醛磷酸脱氢酶（GAPDH）mRNA片段的特异性扩增确定核酸库的量; c）扩增步骤b）的核酸库，使用非特异性有义和反义引物获得核酸序列的明确模式; 和d）使扩增的核酸序列进行凝胶电泳以获得患病和健康的指纹，其中主要与患病组织相关的条带是病理学的标志物。 本发明还涉及用于诊断克罗恩病的RNA表达标志物，其包含3.1kb RNA序列及其用途。

    Target-dependent nucleic acid adapter
    9.
    发明授权
    Target-dependent nucleic acid adapter 有权
    靶依赖性核酸适配器

    公开(公告)号：US09012140B2

    公开(公告)日：2015-04-21

    申请号：US11631689

    申请日：2005-07-06

    Applicant: Jean-Pierre Perreault ,  Luncien Junior Bergeron

    Inventor： Jean-Pierre Perreault ,  Luncien Junior Bergeron

    IPC: C12Q1/68 ,  C12N15/11 ,  A61K48/00 ,  C12N15/113 ,  C07H21/04

    CPC classification number: C12N15/1131 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/123 ,  C12N2310/3519 ,  C12N2320/50

    Abstract: In accordance with the present invention, there is provided a nucleic acid target-dependent adapter linked to a nucleic acid sequence. The adapter comprises linked together a biosensor having a specific sequence complementary to a target sequence of a substrate, the biosensor improving the specificity of the nucleic acid sequence for the substrate, and a blocker stem sequence complementary to a portion of the nucleic acid sequence. In absence of the target sequence of the substrate, the blocker stem sequence forms an intramolecular stem with the nucleic acid sequence linked thereto, preventing exposition of the sequence of the nucleic acid sequence, thus locking the nucleic acid sequence so linked to the adapter in an inactive conformation, and in presence of the target sequence, the blocker stem sequence dissociating from the nucleic acid sequence, thus exposing the nucleic acid sequence linked to the adapter in an active conformation.

    Abstract translation: 根据本发明，提供了与核酸序列连接的核酸靶依赖性衔接子。 适配器包括将具有与底物的靶序列互补的特定序列的生物传感器连接在一起，生物传感器提高了底物的核酸序列的特异性，以及与核酸序列的一部分互补的阻断茎序列。 在不存在底物的靶序列的情况下，阻断剂茎序列形成与其连接的核酸序列的分子内茎，防止核酸序列的序列的显露，从而锁定与适配器连接的核酸序列 在靶序列存在下，阻断茎干序列与核酸序列解离，从而将与衔接子连接的核酸序列以活性构象暴露。

    Nucleic acid enzyme for RNA cleavage
    10.
    发明授权
    Nucleic acid enzyme for RNA cleavage 失效
    用于RNA切割的核酸酶

    公开(公告)号：US07148044B1

    公开(公告)日：2006-12-12

    申请号：US09699667

    申请日：2000-10-30

    Applicant: Jean-Pierre Perreault ,  Sirinart Ananvoranich ,  Daniel Lafontaine

    Inventor： Jean-Pierre Perreault ,  Sirinart Ananvoranich ,  Daniel Lafontaine

    IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC classification number: C12N15/113 ,  A61K38/00 ,  C12N2310/111 ,  C12N2310/123

    Abstract: A method is described for cleaving a nucleic acid substrate with a nucleic acid enzyme at a cleavage site comprising mixing the substrate with the enzyme, wherein the substrate includes a 7 nucleotide sequence with at least 6 nucleotides 3′ to the cleavage site and at least 1 nucleotide 5′ to the cleavage site and of formula: 5′-H′GNNHNN-3′ wherein each N is a nucleotide which may be the same or different, H is a nucleotide selected from the group consisting of A, U, C, and T, and is the site of cleavage, and H′ is a ribonucleotide selected from the group consisting of A, U, and C, wherein (i) the first nucleotide 3′ to the cleavage site is capable of forming a wobble pair with the enzyme, (ii) the second, third, fifth, and sixth nucleotides 3′ to the cleavage site are capable of forming conventional Watson-Crick base pairs with the enzyme, (iii) the fourth nucleotide 3′ to the cleavage site is capable of forming a non-conventional Watson-Crick base pair with the enzyme, and (iv) the first nucleotide 5′ to the cleavage site does not form a base pair with the enzyme; and the enzyme comprises a substrate binding portion which is capable of base pairing to the 6 nucleotides 3′ to the cleavage site of the substrate and which binding portion comprises the sequence: 3′-UNNXNN-5′ wherein each N is a nucleotide which may be the same or different, and X is a nucleotide selected from the group consisting of T, U, A, and G, whereby binding of the substrate to the enzyme effects cleavage of the substrate at the cleavage site.

    Abstract translation: 描述了一种用于在切割位点用核酸酶切割核酸底物的方法，包括将底物与酶混合，其中所述底物包含与切割位点具有至少6个核苷酸3'的7个核苷酸序列和至少1个 核苷酸5'至切割位点，并具有下式：<？in-line-formula description =“In-line formula”end =“lead”？> 5'-H'GNNHNN-3'<？in-line-formula description =“内联式”末端=“尾”→其中每个N是可以相同或不同的核苷酸，H是选自A，U，C和T的核苷酸，并且是 切割位点，H'是选自A，U和C的核糖核苷酸，其中（i）切割位点的第一个核苷酸3'能够与酶形成摆动对，（ii） 切割位点的第二，第三，第五和第六核苷酸3'能够与酶形成常规的沃森 - 克里克碱基对，（iii）第四核 到切割位点的3'能够与酶形成非常规的Watson-Crick碱基对，和（iv）切割位点的第一个核苷酸5'不与酶形成碱基对; 并且该酶包括能够与基底的切割位点的6'核苷酸3'碱基配对的底物结合部分，并且该结合部分包含以下序列：<？in-line-formula description =“In-line Formulas” end =“lead”？> 3'-UNNXNN-5'<？in-line-formula description =“In-line Formulas”end =“tail”？>其中每个N是可以相同或不同的核苷酸， 并且X是选自T，U，A和G的核苷酸，由此底物与酶的结合在切割位点处影响底物的切割。

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