公开(公告)号：US6090616A

公开(公告)日：2000-07-18

申请号：US922865

申请日：1997-09-03

Applicant: Richard Anthony Wisdom ,  Caroline Susan Lee ,  Robert Christopher Brown

Inventor： Richard Anthony Wisdom ,  Caroline Susan Lee ,  Robert Christopher Brown

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/86 ,  C12N15/55 ,  C12P13/02 ,  C12P41/00 ,  C12R1/01 ,  C12P17/10 ,  C12P19/38 ,  C12P37/00

CPC classification number: C12N9/86 ,  C12P41/006

Abstract: A lactamase enzyme having good stability, capable of hydrolysing an enatiomer of the bicyclic lactam, 2-azabicyclo[2.2.1]hept-5-en-3-one, to give (-) lactam and (+) amino acid, has been found in a strain of Comamonas acidivorans. The enzyme has been isolated and cloned, and its structure identified.

Abstract translation: 具有良好稳定性的能够水解双环内酰胺-2-氮杂双环[2.2.1]庚-5-烯-3-酮的对映体以产生（ - ）内酰胺和（+）氨基酸的内酰胺酶已经被 发现在一个Comamonas酸运动的菌株。 酶已被分离并克隆，其结构鉴定。

公开(公告)号：US5912164A

公开(公告)日：1999-06-15

申请号：US854097

申请日：1997-05-09

Applicant: Julie Belinda Hazel Warneck ,  Richard Anthony Wisdom

Inventor： Julie Belinda Hazel Warneck ,  Richard Anthony Wisdom

IPC: C12N9/18 ,  C12P7/02 ,  C12P7/40 ,  C12P41/00 ,  C07C1/04

CPC classification number: C12N9/18 ,  C12P41/003 ,  C12P41/004 ,  C12P7/02 ,  C12P7/40 ,  Y10S435/911

Abstract: An organism of the genus Ophiostoma or Ceratocystis, or a material having enzymatic activity derived therefrom, is used as a stereo-specific agent, in a biotransformation reaction, for the production of an enantiomeric acid or alcohol from a mixture of enantiomers of an ester thereof.

Abstract translation: 在生物转化反应中使用Ophiostoma属或Ceratocystis属的生物或其衍生的具有酶活性的物质作为立体特异性试剂，用于从其酯的对映异构体的混合物制备对映异构体酸或醇 。

公开(公告)号：US5733754A

公开(公告)日：1998-03-31

申请号：US615193

申请日：1996-04-08

Applicant: Richard Anthony Wisdom ,  Caroline Susan Lee ,  Paul Maurice Ricaud

Inventor： Richard Anthony Wisdom ,  Caroline Susan Lee ,  Paul Maurice Ricaud

IPC: C12N1/21 ,  C12N9/80 ,  C12P17/12 ,  C12P41/00 ,  C12R1/05 ,  C12P13/04 ,  C12N1/20 ,  C12N9/78 ,  C12N11/00

CPC classification number: C12R1/05 ,  C12N9/80 ,  C12P17/12 ,  C12P41/007

Abstract: An enzyme is provided which has acylase activity capable of hydrolyzing N-acetyl-(R,S)-pipecolic acid stereoselectively to give (S)-pipecolic acid, wherein this activity is greater than that on N-acetyl-S-proline, at pH 7.5, 25.degree. C., and substrate concentration 20 g/l, in 75 mM Tris buffer. The enzyme is obtainable from the species of Alcaligenes denitrificans deposited as NCIMB 40587. A microorganism having therein the enzyme also is provided, as is a process for preparing (S)-pipecolic acid comprising contacting a mixture of enantiomers of an N-acyl-pipe colic acid with the enzyme or microorganism.

Abstract translation: PCT No.PCT / GB94 / 02259 Sec。 371日期：1996年4月8日 102（e）日期1996年4月8日PCT 1994年10月14日PCT PCT。 公开号WO95 / 10604 日期1995年4月20日提供具有能够立体选择性水解N-乙酰基 - （R，S） - 十二酸的酰基转移酶活性的酶，得到（S） - 十二酸，其中该活性大于N-乙酰基 - S-脯氨酸，pH 7.5,25℃，底物浓度20g / l，在75mM Tris缓冲液中。 该酶可从作为NCIMB 40587沉积的嗜碱性杆菌属物种获得。还提供了其中还含有该酶的微生物，制备（S） - 癸二酸的方法包括使N-酰基管的对映异构体的混合物 肠酸与酶或微生物。

公开(公告)号：US06423522B1

公开(公告)日：2002-07-23

申请号：US09510949

申请日：2000-02-22

Applicant: Richard Anthony Wisdom ,  Caroline Susan Lee ,  Robert Christopher Brown

Inventor： Richard Anthony Wisdom ,  Caroline Susan Lee ,  Robert Christopher Brown

IPC: C12N914

CPC classification number: C12N9/86 ,  C12P41/006

Abstract: A lactamase enzyme having good stability, capable of hydrolysing an enantiomer of the bicyclic lactam, 2-azabicyclo[2.2.1]hept-5-en-3-one, to give (−) lactam and (+) amino acid, has been found in a strain of Comamonas acidivorans. The enzyme has been isolated and cloned, and its structure identified.

Abstract translation: 具有良好稳定性的能够水解双环内酰胺2-氮杂双环[2.2.1]庚-5-烯-3-酮的对映体以得到（ - ）内酰胺和（+）氨基酸的内酰胺酶已经被 发现在一个Comamonas酸运动的菌株。 酶已被分离并克隆，其结构鉴定。