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    Dry multilayer analytical elements for assaying transaminases
    1.
    发明授权
    Dry multilayer analytical elements for assaying transaminases 失效
    用于测定转氨酶的干多层分析元件

    公开(公告)号：US5462858A

    公开(公告)日：1995-10-31

    申请号：US175013

    申请日：1993-12-29

    Applicant: Marsha D. Bale Oenick ,  Richard L. Detwiler ,  Jon N. Eikenberry ,  John W. H. Sutherland

    Inventor： Marsha D. Bale Oenick ,  Richard L. Detwiler ,  Jon N. Eikenberry ,  John W. H. Sutherland

    IPC: G01N31/22 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/52 ,  G01N21/78 ,  G01N33/50 ,  G01N33/52 ,  C12Q1/28 ,  G01N21/25

    CPC classification number: G01N33/526 ,  Y10S435/805

    Abstract: A dry multilayer analytical element for assaying transaminases wherein the analytical element comprises:a) a transaminase substrate;b) reagents that generate pyruvate in the presence of a transaminase and the transaminase substrate;c) pyruvate oxidase and cofactors for pyruvate oxidase; andd) reagents that generate a color in proportion to a concentration of hydrogen peroxide;and wherein the analytical element has, in the following order, a spreading layer, a reagent layer, and a support whereini) the spreading layer contains the transaminase substrate and a material selected from the group consisting of polymer beads, barium sulfate, titanium oxide, and mixtures thereof;ii) the reagent layer contains the pyruvate oxidase; andiii) both a hardened gelatin layer and a subbing layer separate the reagent layer from the spreading layer.

    Abstract translation: 一种用于测定转氨酶的干燥多层分析元件，其中所述分析元件包括：a）转氨酶底物; b）在转氨酶和转氨酶底物存在下产生丙酮酸的试剂; c）丙酮酸氧化酶和丙酮酸氧化酶的辅因子; 和d）产生与过氧化氢浓度成比例的颜色的试剂; 并且其中所述分析元件按以下顺序具有扩散层，试剂层和载体，其中所述扩散层含有所述转氨酶底物和选自聚合物珠粒，硫酸钡，氧化钛 ，及其混合物; ii）试剂层含有丙酮酸氧化酶; 和iii）硬化的明胶层和底层都将试剂层与扩散层分开。

    Multilayer analytical element containing primary amine buffer and method for the determination of ethanol
    3.
    发明授权
    Multilayer analytical element containing primary amine buffer and method for the determination of ethanol 失效
    含有伯胺缓冲液的多层分析元素和乙醇测定方法

    公开(公告)号：US5416004A

    公开(公告)日：1995-05-16

    申请号：US5683

    申请日：1993-01-19

    Applicant: Richard L. Detwiler

    Inventor： Richard L. Detwiler

    IPC: C12Q1/00 ,  C12Q1/32 ,  G01N33/497 ,  G01N33/52 ,  G01N33/98 ,  C12Q1/26 ,  G01N21/00 ,  G01N21/77 ,  G01N33/00

    CPC classification number: G01N33/98 ,  G01N33/4972 ,  G01N33/526 ,  G01N2800/307 ,  Y10T436/203332 ,  Y10T436/204165

    Abstract: A multilayer analytical element has been prepared for accurate and rapid colorimetric determination of ethanol in aqueous specimens using alcohol dehydogenase and an oxidized nicotinamide coenzyme. The element includes three reagent layers beneath a porous spreading layer. The middle reagent layer has a crosslinked hydrophilic binder, while the other two reagent layers have uncrosslinked binders. In addition, both uncrosslinked reagent layers include a relatively high amount of a buffer having a primary amine, which buffer maintains the layer pH at from about 8 to about 10. Alcohol dehydrogenase is in the reagent layer next to the porous spreading layer.

    Abstract translation: 已经制备了一种多层分析元件，用于使用醇脱水酶和氧化烟酰胺辅酶对含水样品中的乙醇进行准确和快速的比色测定。 该元件包括在多孔扩散层下面的三个试剂层。 中间试剂层具有交联的亲水性粘合剂，而另外两个试剂层具有未交联的粘合剂。 此外，两个未交联的试剂层都包括相对较高量的具有伯胺的缓冲液，该缓冲剂将层的pH保持在约8至约10℃。醇脱氢酶位于多孔扩散层旁边的试剂层中。

    Method for the determination of bilirubin and an element useful therein
    6.
    发明授权
    Method for the determination of bilirubin and an element useful therein 失效
    胆红素测定方法及其中有用的元素

    公开(公告)号：US4788153A

    公开(公告)日：1988-11-29

    申请号：US106759

    申请日：1987-10-08

    Applicant: Richard L. Detwiler ,  David M. Taylor

    Inventor： Richard L. Detwiler ,  David M. Taylor

    IPC: G01N33/52 ,  G01N21/75 ,  G01N33/72

    CPC classification number: G01N33/526 ,  Y10T436/146666

    Abstract: A colorimetric assay for the determination of conjugated, unconjugated or total bilirubin in biological fluids can be carried out with an improved analytical element. This element comprises a support having thereon a substantially gelatin-free mordant layer comprising a positively-charged interactive mordant having at least one binding site for bilirubin, a radiation-blocking layer, and a porous spreading layer. This element does not contain any colorimetric or fluorometric interactive compositions for bilirubin other than the mordant.

    Abstract translation: 用于测定生物液体中共轭，非共轭或总胆红素的比色测定法可以用改进的分析元件进行。 该元件包括其上具有基本上无明胶的媒染剂层的载体，其包含具有至少一个胆红素结合位点，辐射阻挡层和多孔扩散层的带正电荷的交互媒染剂。 该元素不包含除媒染物之外的任何比色或荧光交互组合物用于胆红素。

    Overcoat compositions and ion-selective electrodes for ionic analyte determinations
    8.
    发明授权
    Overcoat compositions and ion-selective electrodes for ionic analyte determinations 失效
    用于离子分析物测定的外涂层组合物和离子选择性电极

    公开(公告)号：US4505801A

    公开(公告)日：1985-03-19

    申请号：US569695

    申请日：1984-01-10

    Applicant: Richard L. Detwiler ,  Brooke P. Schlegel ,  Thomas R. Kissel

    Inventor： Richard L. Detwiler ,  Brooke P. Schlegel ,  Thomas R. Kissel

    IPC: G01N27/28 ,  G01N27/30 ,  G01N27/333

    CPC classification number: G01N27/3335 ,  Y10T436/25125

    Abstract: An overcoat composition for use in ion-selective electrodes comprises: (a) a discontinuous liquid phase comprising an oleophilic solvent dispersed within a continuous phase comprising a hydrophilic binder; (b) a complexing agent useful for extracting oleophilic anions; (c) a buffer which provides a pH in the range of from about 7.5 to about 9.5 under conditions of use; and (d) a nucleating agent. This overcoat composition is particularly useful in ion-selective electrodes for determination of CO.sub.2.

    Abstract translation: 用于离子选择性电极的外涂层组合物包括：（a）包含分散在包含亲水性粘合剂的连续相中的亲油溶剂的不连续液相; （b）可用于提取亲油性阴离子的络合剂; （c）在使用条件下提供约7.5至约9.5范围内的pH的缓冲液; 和（d）成核剂。 该外涂层组合物特别适用于用于测定二氧化碳的离子选择性电极。

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