Process for expression of foreign genes in methylotrophic bacteria through chromosomal integration
    2.
    发明申请
    Process for expression of foreign genes in methylotrophic bacteria through chromosomal integration 审中-公开
    通过染色体整合在甲基营养细菌中表达外源基因的方法

    公开(公告)号:US20050287625A1

    公开(公告)日:2005-12-29

    申请号:US11070080

    申请日:2005-03-02

    申请人: Edward Miller Rick Ye

    发明人: Edward Miller Rick Ye

    摘要: Provided is a method for expressing an introduced gene or genes in a C1 metabolizing microorganism host wherein the gene(s) are integrated into the tig region of the chromosome. This method provides high level expression in a stable manner in which growth rate of the host strain is not highly affected and a selection marker is not required. The use of this method for expressing carotenoid biosynthetic genes and resulting production of canthaxanthin is also described.

    摘要翻译: 提供了在C1代谢微生物宿主中表达引入的基因或基因的方法,其中所述基因整合到染色体的tig区域中。 该方法以稳定的方式提供高水平的表达,其中宿主菌株的生长速率不受高度影响并且不需要选择标记。 还描述了使用这种方法来表达类胡萝卜素生物合成基因并导致角黄素的产生。

    Mutant carotenoid ketolase
    4.
    发明申请
    Mutant carotenoid ketolase 失效
    突变类胡萝卜素酮醇酶

    公开(公告)号:US20070231854A1

    公开(公告)日:2007-10-04

    申请号:US11395644

    申请日:2006-03-31

    CPC分类号: C12P23/00 C12N9/0069

    摘要: CrtW carotenoid ketolases are provided useful for the production of astaxanthin. The ketolases genes of the present invention exhibit improved ketolase activity when converting cyclic hydroxylated carotenoid intermediates into astaxanthin. Recombinant expression of the present carotenoid ketolases in host cell producing cyclic hydroxylated carotenoid intermediates enabled increased production of astaxanthin.

    摘要翻译: CrtW类胡萝卜素酮酶可用于生产虾青素。 当将环状羟基化类胡萝卜素中间体转化成虾青素时,本发明的酮糖酶基因表现出改善的酮糖酶活性。 目前的类胡萝卜素酮酶在宿主细胞产生的环状羟基化类胡萝卜素中间体中的重组表达能够增加虾青素的产生。

    GENES ENCODING DENITRIFICATION ENZYMES
    7.
    发明申请
    GENES ENCODING DENITRIFICATION ENZYMES 失效
    基因编码DENITRIFICATION ENZYMES

    公开(公告)号:US20050084945A1

    公开(公告)日:2005-04-21

    申请号:US09789161

    申请日:2001-02-20

    摘要: This invention relates to the isolation of nucleic acid fragments from Methylamons sp. that encode enzymes involved in denitrification. The enzymes are useful in denitrification reactions and for the identification of other denitrifying bacteria. In addition, this invention also relates to the construction of chimeric genes encoding all or a substantial portion of the present genes in sense or antisense orientation, wherein the expression of the chimeric genes results in production of altered levels of the present gene products in the recombinant host.

    摘要翻译: 本发明涉及从Methylamons sp。分离核酸片段。 编码涉及脱氮的酶。 这些酶可用于脱氮反应和鉴定其他反硝化细菌。 此外,本发明还涉及在正义或反义方向上编码本基因的全部或大部分的嵌合基因的构建,其中嵌合基因的表达导致重组中本发明基因产物水平的变化 主办。