Method and apparatus for detection or identification of DNA
    1.
    发明申请
    Method and apparatus for detection or identification of DNA 审中-公开
    用于检测或鉴定DNA的方法和装置

    公开(公告)号:US20050048501A1

    公开(公告)日:2005-03-03

    申请号:US10653365

    申请日:2003-09-02

    IPC分类号: C12P19/34 C12Q1/68

    摘要: DNA is detected using complementary RNA probes and an enzyme that attacks and hydrolyzes the RNA probes only when it has hybridized with target DNA. A low concentration of target DNA can therefore successively hydrolyze a larger amount of RNA whose loss may then be detected to indirectly determine the presence of the target DNA.

    摘要翻译: 使用互补RNA探针和仅在与目标DNA杂交时才攻击和水解RNA探针的酶检测DNA。 因此,低浓度的靶DNA可以连续地水解较大量的RNA,然后可以检测其损失以间接测定靶DNA的存在。

    Methods for analysis of a nucleic acid sample
    2.
    发明申请
    Methods for analysis of a nucleic acid sample 审中-公开
    分析核酸样品的方法

    公开(公告)号:US20060211024A1

    公开(公告)日:2006-09-21

    申请号:US11372823

    申请日:2006-03-09

    IPC分类号: C12Q1/68

    摘要: An assay for analysis of a nucleic acid sample is provided. In one embodiment, the assay includes: a) employing an RNA sample to obtain a DNA probe; b) contacting the DNA probe with a substrate containing a surface-immobilized RNA oligonucleotide to produce a surface-immobilized RNA/DNA duplex; and c) detecting RNAseH-dependent cleavage of the surface-immobilized RNA oligonucleotide in the RNA/DNA duplex.

    摘要翻译: 提供了用于分析核酸样品的测定法。 在一个实施方案中,测定包括:a)使用RNA样品获得DNA探针; b)将DNA探针与含有表面固定的RNA寡核苷酸的底物接触以产生表面固定的RNA / DNA双链体; 和c)检测RNA / DNA双链体中表面固定的RNA寡核苷酸的RNAseH依赖性切割。