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公开(公告)号:US20050048501A1
公开(公告)日:2005-03-03
申请号:US10653365
申请日:2003-09-02
申请人: Robert Corn , Terry Goodrich , Hye Lee
发明人: Robert Corn , Terry Goodrich , Hye Lee
CPC分类号: C12Q1/682 , C12Q1/6869 , C12Q2565/628 , C12Q2521/319
摘要: DNA is detected using complementary RNA probes and an enzyme that attacks and hydrolyzes the RNA probes only when it has hybridized with target DNA. A low concentration of target DNA can therefore successively hydrolyze a larger amount of RNA whose loss may then be detected to indirectly determine the presence of the target DNA.
摘要翻译: 使用互补RNA探针和仅在与目标DNA杂交时才攻击和水解RNA探针的酶检测DNA。 因此,低浓度的靶DNA可以连续地水解较大量的RNA,然后可以检测其损失以间接测定靶DNA的存在。
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公开(公告)号:US20060211024A1
公开(公告)日:2006-09-21
申请号:US11372823
申请日:2006-03-09
申请人: Robert Corn , Lloyd Smith , Timothy Burland , Voula Kodoyianni
发明人: Robert Corn , Lloyd Smith , Timothy Burland , Voula Kodoyianni
IPC分类号: C12Q1/68
CPC分类号: B82Y15/00 , B82Y30/00 , C12Q1/6834 , C12Q2561/108 , C12Q2537/149 , C12Q2533/101
摘要: An assay for analysis of a nucleic acid sample is provided. In one embodiment, the assay includes: a) employing an RNA sample to obtain a DNA probe; b) contacting the DNA probe with a substrate containing a surface-immobilized RNA oligonucleotide to produce a surface-immobilized RNA/DNA duplex; and c) detecting RNAseH-dependent cleavage of the surface-immobilized RNA oligonucleotide in the RNA/DNA duplex.
摘要翻译: 提供了用于分析核酸样品的测定法。 在一个实施方案中,测定包括:a)使用RNA样品获得DNA探针; b)将DNA探针与含有表面固定的RNA寡核苷酸的底物接触以产生表面固定的RNA / DNA双链体; 和c)检测RNA / DNA双链体中表面固定的RNA寡核苷酸的RNAseH依赖性切割。
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公开(公告)号:US20080113446A1
公开(公告)日:2008-05-15
申请号:US12015186
申请日:2008-01-16
申请人: Alastair Wark , Hye Jin , Robert Corn , Voula Kodoyianni
发明人: Alastair Wark , Hye Jin , Robert Corn , Voula Kodoyianni
CPC分类号: G01N21/554 , B82Y30/00 , G01N21/648 , Y10S435/808 , Y10S435/973 , Y10S436/805 , Y10S436/809 , Y10T156/1089
摘要: A substrate for testing a carrier liquid for biomolecules using SPR or other techniques provides a metallic island surrounded by a hydrophobic layer, the islands being a location for the attachment of probe molecules. The hydrophobic layer may also be a dielectric material providing improved sensitivity in SPR imaging.
摘要翻译: 用于使用SPR或其他技术测试生物分子载体液体的基底提供了由疏水层包围的金属岛,所述岛是用于附着探针分子的位置。 疏水层也可以是在SPR成像中提供改善的灵敏度的电介质材料。
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公开(公告)号:US20060066249A1
公开(公告)日:2006-03-30
申请号:US11231626
申请日:2005-09-21
申请人: Alastair Wark , Hye Lee , Robert Corn , Voula Kodoyianni
发明人: Alastair Wark , Hye Lee , Robert Corn , Voula Kodoyianni
CPC分类号: G01N21/554 , B82Y30/00 , G01N21/648 , Y10S435/808 , Y10S435/973 , Y10S436/805 , Y10S436/809 , Y10T156/1089
摘要: A substrate for testing a carrier liquid for biomolecules using SPR or other techniques provides a metallic island surrounded by a hydrophobic layer, the islands being a location for the attachment of probe molecules. The hydrophobic layer may also be a dielectric material providing improved sensitivity in SPR imaging.
摘要翻译: 用于使用SPR或其他技术测试生物分子载体液体的基底提供了由疏水层包围的金属岛,所述岛是用于附着探针分子的位置。 疏水层也可以是在SPR成像中提供改善的灵敏度的电介质材料。
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