Methods for analysis of a nucleic acid sample
    3.
    发明申请
    Methods for analysis of a nucleic acid sample 审中-公开
    分析核酸样品的方法

    公开(公告)号:US20060211024A1

    公开(公告)日:2006-09-21

    申请号:US11372823

    申请日:2006-03-09

    IPC分类号: C12Q1/68

    摘要: An assay for analysis of a nucleic acid sample is provided. In one embodiment, the assay includes: a) employing an RNA sample to obtain a DNA probe; b) contacting the DNA probe with a substrate containing a surface-immobilized RNA oligonucleotide to produce a surface-immobilized RNA/DNA duplex; and c) detecting RNAseH-dependent cleavage of the surface-immobilized RNA oligonucleotide in the RNA/DNA duplex.

    摘要翻译: 提供了用于分析核酸样品的测定法。 在一个实施方案中,测定包括:a)使用RNA样品获得DNA探针; b)将DNA探针与含有表面固定的RNA寡核苷酸的底物接触以产生表面固定的RNA / DNA双链体; 和c)检测RNA / DNA双链体中表面固定的RNA寡核苷酸的RNAseH依赖性切割。