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    Fractionation of Macro-Molecules Using Asymmetric Pulsed Field Electrophoresis
    1.
    发明申请
    Fractionation of Macro-Molecules Using Asymmetric Pulsed Field Electrophoresis 审中-公开
    使用不对称脉冲场电泳分离大分子

    公开(公告)号：US20090014332A1

    公开(公告)日：2009-01-15

    申请号：US12181827

    申请日：2008-07-29

    申请人： Lotien Richard Huang ,  James Christopher Sturm ,  Robert Hamilton Austin

    发明人： Lotien Richard Huang ,  James Christopher Sturm ,  Robert Hamilton Austin

    IPC分类号： B01D57/02

    CPC分类号： C12N15/101 ,  Y10T436/10 ,  Y10T436/11 ,  Y10T436/25

    摘要： A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.

    摘要翻译： 提供了用于分离带电大分子如DNA的方法和装置。 将DNA溶液加载到包括障碍物阵列的矩阵中。 施加具有不同取向的两个不同场的交变电场。 交变电场是不对称的，因为一个场的持续时间或强度比另一个场强更强，否则是不对称的。 因此，DNA分子根据位点进行分级，并被驱动到基质的远侧，其中分离的DNA被回收。 分馏电场可用于负载和回收DNA以连续操作过程。

    Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules
    4.
    发明授权
    Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules 有权
    用于产生用于快速分离分子的电场和流动分布的方法和装置

    公开(公告)号：US07597791B2

    公开(公告)日：2009-10-06

    申请号：US10147370

    申请日：2002-05-15

    申请人： Lotien Richard Huang ,  James Christopher Sturm ,  Robert Hamilton Austin

    发明人： Lotien Richard Huang ,  James Christopher Sturm ,  Robert Hamilton Austin

    IPC分类号： G01N27/453

    CPC分类号： B01D57/02 ,  G01N27/44773

    摘要： A method and apparatus for generating tunable, uniform electric fields in fluidic applications for rapid separation of molecules, such as DNA, is provided. A region receives the molecules to be separated, the molecules being injected into the region by an injection channel connected thereto. Fluidic microchannels or resistor arrays connected to sides of the region inject currents into the region and produce electric fields in the region that can be oriented at any angle. The electric fields can separate the molecules according to size, and can be used to move or manipulate the molecules within the region. Further, the molecules can be separated by controlling fluid flows within the region to manipulate the molecules. One or more reservoirs can be attached to the fluidic microchannels for collecting the molecules after separation, movement, or manipulation.

    摘要翻译： 提供了一种用于在用于快速分离分子（例如DNA）的流体应用中产生可调谐均匀电场的方法和装置。 区域接收要分离的分子，分子通过与其连接的注入通道注入该区域。 连接到区域侧面的流体微通道或电阻器阵列将电流注入该区域，并在可以任意角度定向的区域中产生电场。 电场可以根据大小分离分子，并且可以用于移动或操纵该区域内的分子。 此外，可以通过控制区域内的流体流动来分离分子以操纵分子。 可以将一个或多个储存器连接到流体微通道，用于在分离，移动或操纵之后收集分子。