公开(公告)号：US20090123910A1

公开(公告)日：2009-05-14

申请号：US11985547

申请日：2007-11-14

申请人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf

发明人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf

IPC分类号： C12Q1/70 ,  C12P21/00 ,  G01N33/50 ,  C12Q1/00 ,  G01N33/567

CPC分类号： G01N33/54393 ,  C07K1/145 ,  C12N15/52 ,  C12N2710/20022 ,  G01N1/4044 ,  G01N33/56983 ,  G01N2333/025 ,  G01N2333/16 ,  G01N2469/10

摘要： Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.

摘要翻译： 提供了从细胞如含有病毒蛋白的细胞或细胞样品中产生蛋白质提取物的方法。 一般来说，所述方法可以包括：将细胞的pH增加到至少约pH 10.0的pH以产生中间体组合物，然后在非离子洗涤剂如聚氧乙烯烷基醚的存在下中和 中间体组合物的pH以产生蛋白质提取物。 这些方法可以结合用于检测样品中的一种或多种靶蛋白的方法，例如病毒蛋白。 还提供了用于实践主题方法的系统，试剂盒和组合物。

公开(公告)号：US09207240B2

公开(公告)日：2015-12-08

申请号：US11985547

申请日：2007-11-14

申请人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf ,  John Mantlo

发明人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf ,  John Mantlo

IPC分类号： C12N15/52 ,  C07K1/14 ,  G01N33/569

CPC分类号： G01N33/54393 ,  C07K1/145 ,  C12N15/52 ,  C12N2710/20022 ,  G01N1/4044 ,  G01N33/56983 ,  G01N2333/025 ,  G01N2333/16 ,  G01N2469/10

摘要： Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.

公开(公告)号：US20080269115A1

公开(公告)日：2008-10-30

申请号：US11570528

申请日：2005-06-16

申请人： Robert J. Beadenkopf

发明人： Robert J. Beadenkopf

IPC分类号： A61K38/16 ,  C07K14/165 ,  C07K7/06 ,  C07K7/08 ,  C07K16/08 ,  G01N33/53 ,  A61P31/12 ,  A61K38/08 ,  A61K38/10

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/20022

摘要： Potentially antigenic, conserved and specific SARS-CoV peptides are disclosed. The disclosed polypeptides may be used in a variety of diagnostic and treatment methods for SARS infection.

摘要翻译： 公开了潜在的抗原性，保守和特异性SARS-CoV肽。 所公开的多肽可用于SARS感染的多种诊断和治疗方法。