公开(公告)号：US5776710A

公开(公告)日：1998-07-07

申请号：US771507

申请日：1996-12-23

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

IPC分类号： G01N33/543 ,  B01L3/14 ,  G01N33/49 ,  G01N33/537 ,  G01N33/569 ,  G01N33/58 ,  G01N33/558

CPC分类号： B01L3/5021 ,  G01N33/491 ,  G01N33/5375 ,  G01N33/56972 ,  G01N33/585 ,  Y10S435/81 ,  Y10S435/967 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/81 ,  Y10S436/824 ,  Y10S436/829 ,  Y10T436/111666

摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

摘要翻译： 可以通过将透明管中的血液样品离心来诊断患者的健康，该管包含一个或多个不同密度的浮体，插入物，脂质体或塑料珠的主体或组。 每个密度定义的身体携带分析物 - 捕获结合材料，例如抗原或抗体，其对靶分析物是特异性的，或靶分析物上的其它特异性高亲和力结合位点，其目标分析物可能在待测试的血液或其他样品中; 并且其分析物的水平表示患者的健康。 将至少一种对靶分析物上的表位或其他特异性高亲和力结合位点特异性的标记结合物质加入到样品中，以便在样品中形成标记的结合材料/分析物/身体复合物。 离心后，复合物将根据身体或身体的相应密度沉淀在管中的不同区域; 并且复合层的标签发射程度可以使得要进行样品的定性和/或定量分析。 通过在离心步骤期间样品的上升或下降组分的洗涤作用，未结合的标记结合材料将从复合层分离。 因此，未结合的标签结合材料不会影响分析。

公开(公告)号：US5635362A

公开(公告)日：1997-06-03

申请号：US247336

申请日：1994-05-23

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Alvydas J. Ozinskas

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Alvydas J. Ozinskas

IPC分类号： G01N33/543 ,  B01L3/14 ,  G01N33/49 ,  G01N33/537 ,  G01N33/569 ,  G01N33/58 ,  G01N33/558

CPC分类号： B01L3/5021 ,  G01N33/491 ,  G01N33/5375 ,  G01N33/56972 ,  G01N33/585 ,  Y10S435/81 ,  Y10S435/967 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/81 ,  Y10S436/824 ,  Y10S436/829 ,  Y10T436/111666

摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

摘要翻译： 可以通过将透明管中的血液样品离心来诊断患者的健康，该管包含一个或多个不同密度的浮体，插入物，脂质体或塑料珠的主体或组。 每个密度定义的身体携带分析物 - 捕获结合材料，例如抗原或抗体，其对靶分析物是特异性的，或靶分析物上的其它特异性高亲和力结合位点，其目标分析物可能在待测试的血液或其他样品中; 并且其分析物的水平表示患者的健康。 将至少一种对靶分析物上的表位或其他特异性高亲和力结合位点特异性的标记结合物质加入到样品中，以便在样品中形成标记的结合材料/分析物/身体复合物。 离心后，复合物将根据身体或身体的相应密度沉淀在管中的不同区域; 并且复合层的标签发射程度可以使得要进行样品的定性和/或定量分析。 通过在离心步骤期间样品的上升或下降组分的洗涤作用，未结合的标记结合材料将从复合层分离。 因此，未结合的标签结合材料不会影响分析。

公开(公告)号：US5108916A

公开(公告)日：1992-04-28

申请号：US361049

申请日：1989-06-05

申请人： Carrington S. Cobbs ,  Michael J. Barton ,  Lin Peng ,  Animesh Goswami ,  Adrien P. Malick ,  John P. Hamman ,  Gary J. Calton

发明人： Carrington S. Cobbs ,  Michael J. Barton ,  Lin Peng ,  Animesh Goswami ,  Adrien P. Malick ,  John P. Hamman ,  Gary J. Calton

IPC分类号： C12N9/20 ,  C12N9/18 ,  C12N11/08 ,  C12P7/40 ,  C12P7/62 ,  C12P7/64 ,  C12P41/00 ,  C12R1/72

CPC分类号： C12P7/6418 ,  C12N9/18 ,  C12P41/003 ,  C12P41/005 ,  C12P7/40 ,  C12P7/6436 ,  C12P7/6445 ,  C12P7/6454 ,  C12P7/6472

摘要： An immobilized isozyme of Lipase MY or AY from Candida rugosa is used for stereoselectively hydrolyzing racemic mixtures of esters of 2-substituted acids, other than 2-halo propionic acids, transesterifying esters or acids or esterify acids or alcohols, at high enantiomeric excess, in an organic solvent. Immobilization of the isozyme may be carried out in the presence of an organic acid such as stearic acid. The immobilized isozyme may be used with a fatty acid or fatty acid ester that increases stereoselectivity or rate of hydrolysis of a mixture of racemic esters.

公开(公告)号：US09207240B2

公开(公告)日：2015-12-08

申请号：US11985547

申请日：2007-11-14

申请人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf ,  John Mantlo

发明人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf ,  John Mantlo

IPC分类号： C12N15/52 ,  C07K1/14 ,  G01N33/569

CPC分类号： G01N33/54393 ,  C07K1/145 ,  C12N15/52 ,  C12N2710/20022 ,  G01N1/4044 ,  G01N33/56983 ,  G01N2333/025 ,  G01N2333/16 ,  G01N2469/10

摘要： Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.

公开(公告)号：US5834217A

公开(公告)日：1998-11-10

申请号：US763858

申请日：1996-12-11

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Leon W. M. M. Terstappen ,  Kristen L. Manion ,  Rodolfo R. Rodriguez ,  Adrien P. Malick ,  Subhash Dhanesar ,  Stephen J. Lovell ,  Alvydas J. Ozinskas

IPC分类号： G01N33/543 ,  B01L3/14 ,  G01N33/49 ,  G01N33/537 ,  G01N33/569 ,  G01N33/58 ,  G01N33/546

CPC分类号： B01L3/5021 ,  G01N33/491 ,  G01N33/5375 ,  G01N33/56972 ,  G01N33/585 ,  Y10S435/81 ,  Y10S435/967 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/81 ,  Y10S436/824 ,  Y10S436/829 ,  Y10T436/111666

摘要： A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

公开(公告)号：US20090123910A1

公开(公告)日：2009-05-14

申请号：US11985547

申请日：2007-11-14

申请人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf

发明人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf

IPC分类号： C12Q1/70 ,  C12P21/00 ,  G01N33/50 ,  C12Q1/00 ,  G01N33/567

CPC分类号： G01N33/54393 ,  C07K1/145 ,  C12N15/52 ,  C12N2710/20022 ,  G01N1/4044 ,  G01N33/56983 ,  G01N2333/025 ,  G01N2333/16 ,  G01N2469/10

摘要： Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.

摘要翻译： 提供了从细胞如含有病毒蛋白的细胞或细胞样品中产生蛋白质提取物的方法。 一般来说，所述方法可以包括：将细胞的pH增加到至少约pH 10.0的pH以产生中间体组合物，然后在非离子洗涤剂如聚氧乙烯烷基醚的存在下中和 中间体组合物的pH以产生蛋白质提取物。 这些方法可以结合用于检测样品中的一种或多种靶蛋白的方法，例如病毒蛋白。 还提供了用于实践主题方法的系统，试剂盒和组合物。