公开(公告)号：US20050009008A1

公开(公告)日：2005-01-13

申请号：US10617569

申请日：2003-07-11

Applicant: Robin Robinson ,  Peter Pushko

Inventor： Robin Robinson ,  Peter Pushko

IPC: A61K39/145 ,  C07H21/04 ,  C07K14/11 ,  C12N7/01 ,  C12N7/04 ,  C12Q1/70 ,  C12N15/09 ,  C12N15/70 ,  C12N15/74 ,  C07K17/00 ,  C07K14/00 ,  C12N15/87 ,  C07K1/00 ,  A61K39/12 ,  C12N15/63 ,  C12N15/85

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/575 ,  A61K2039/70 ,  C07H21/04 ,  C07K14/005 ,  C12N7/00 ,  C12N2760/16023 ,  C12N2760/16034 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134

Abstract: Recombinant influenza virus proteins, including influenza capsomers, subviral particles, virus-like particles (VLP), VLP complexes, and/or any portions of thereof, are provided as a vaccine for influenza viruses. The invention is based on the combination of two vaccine technologies: (1) intrinsically safe recombinant vaccine technology, and (2) highly immunogenic, self-assembled protein macromolecules embedded in plasma membranes and comprised of multiple copies of influenza virus structural proteins exhibiting neutralizing epitopes in native conformations. More specifically, this invention relates to the design and production of functional homotypic and heterotypic recombinant influenza virus-like particles (VLPs) comprised of recombinant structural proteins of human influenza virus type A/Sydney/5/94 (H3N2) and/or avian influenza virus type A/Hong Kong/1073/99 (H9N2) in baculovirus-infected insect cells and their application as a vaccine in the prevention of influenza infections and as a laboratory reagent for virus structural studies and clinical diagnostics.

Abstract translation: 提供重组流感病毒蛋白，包括流行性感冒病毒，亚病毒颗粒，病毒样颗粒（VLP），VLP复合物和/或其任何部分作为流感病毒疫苗。 本发明基于两种疫苗技术的组合：（1）本质安全的重组疫苗技术，和（2）高度免疫原性的，自组装的蛋白质大分子，其包埋在质膜中，并且包含表现中和表位的流感病毒结构蛋白的多个拷贝 在原生构象。 更具体地说，本发明涉及由人流感病毒A型/悉尼/ 5/94（H3N2）和/或禽流感的重组结构蛋白构成的功能同型和异型重组流感病毒样颗粒（VLPs）的设计和生产 病毒A型/香港/ 1073/99（H9N2）在杆状病毒感染昆虫细胞中的应用，并将其作为疫苗用于预防流感感染和作为病毒结构研究和临床诊断的实验室试剂。

公开(公告)号：US20050118191A1

公开(公告)日：2005-06-02

申请号：US10918337

申请日：2004-08-13

Applicant: Robin Robinson ,  Vittoria Cioce

Inventor： Robin Robinson ,  Vittoria Cioce

IPC: A61K39/12 ,  C07K14/025 ,  C07K14/14 ,  C12N5/06 ,  C12N7/00 ,  C12N7/01 ,  C12N7/04 ,  C12N15/86 ,  C12P21/04

CPC classification number: C07K14/005 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2510/02 ,  C12N2710/14143 ,  C12N2710/20022 ,  C12N2710/20023 ,  C12N2720/12322

Abstract: Chimeric virus-like particles that exhibit conformational antigenic epitopes capable of eliciting neutralizing antibodies are disclosed herein. The chimeric virus-like particles of the invention comprise a recombinant viral capsid protein that encapsulates a recombinant viral protein during self-assembly into a chimeric virus-like particle, wherein the chimeric virus-like particle exhibits confirmational antigenic epitopes capable of eliciting neutralizing antibodies. Pharmaceutical compositions, vaccines, and diagnostic test kits containing the chimeric virus-particles are also provided.

Abstract translation: 本文公开了表现出能引发中和抗体的构象抗原表位的嵌合病毒样颗粒。 本发明的嵌合病毒样颗粒包含重组病毒衣壳蛋白，其在自组装期间将重组病毒蛋白包封成嵌合病毒样颗粒，其中嵌合病毒样颗粒显示能够引发中和抗体的确认性抗原表位。 还提供了含有嵌合病毒颗粒的药物组合物，疫苗和诊断测试试剂盒。

公开(公告)号：US20130039938A1

公开(公告)日：2013-02-14

申请号：US13280043

申请日：2011-10-24

Applicant: Gale SMITH ,  Yingyun Wu ,  Michael Massare ,  Peter Pushko ,  Margret Nathan ,  Thomas Kort ,  Robin Robinson

Inventor： Gale SMITH ,  Yingyun Wu ,  Michael Massare ,  Peter Pushko ,  Margret Nathan ,  Thomas Kort ,  Robin Robinson

IPC: A61K39/145 ,  C12P21/02 ,  C12N9/24 ,  C07K14/11 ,  A61P37/04

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C07K2319/01 ,  C07K2319/03 ,  C12N9/2402 ,  C12N2710/14143 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16171 ,  C12N2760/16222 ,  C12N2760/16223 ,  C12N2760/16251 ,  C12N2770/20022 ,  C12N2770/20023 ,  C12N2770/20051 ,  C12N2800/22 ,  C12Y302/01018

Abstract: This invention discloses a method of increasing production of virus-like particles comprising expressing an avian influenza matrix protein. The invention also comprises methods of making and using said VLPs.

公开(公告)号：US20060263804A1

公开(公告)日：2006-11-23

申请号：US11372466

申请日：2006-03-10

Applicant: Robin Robinson ,  Peter Pushko

Inventor： Robin Robinson ,  Peter Pushko

IPC: C12Q1/68 ,  C12P21/06

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/575 ,  A61K2039/70 ,  C07H21/04 ,  C07K14/005 ,  C12N7/00 ,  C12N2760/16023 ,  C12N2760/16034 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134

Abstract: Recombinant influenza virus proteins, including influenza capsomers, subviral particles, virus-like particles (VLP), VLP complexes, and/or any portions of thereof, are provided as a vaccine for influenza viruses. The invention is based on the combination of two vaccine technologies: (1) intrinsically safe recombinant vaccine technology, and (2) highly immunogenic, self-assembled protein macromolecules embedded in plasma membranes and comprised of multiple copies of influenza virus structural proteins exhibiting neutralizing epitopes in native conformations. More specifically, this invention relates to the design and production of functional homotypic and heterotypic recombinant influenza virus-like particles (VLPs) comprised of recombinant structural proteins of human influenza virus type A/Sydney/5/94 (H3N2) and/or avian influenza virus type A/Hong Kong/1073/99 (H9N2) in baculovirus-infected insect cells and their application as a vaccine in the prevention of influenza infections and as a laboratory reagent for virus structural studies and clinical diagnostics.

公开(公告)号：US08992939B2

公开(公告)日：2015-03-31

申请号：US13280043

申请日：2011-10-24

Applicant: Gale Smith ,  Yingyun Wu ,  Michael Massare ,  Peter Pushko ,  Margret Nathan ,  Thomas Kort ,  Robin Robinson

Inventor： Gale Smith ,  Yingyun Wu ,  Michael Massare ,  Peter Pushko ,  Margret Nathan ,  Thomas Kort ,  Robin Robinson

IPC: C12N7/00 ,  C12N7/04 ,  A61K39/145 ,  A61K39/12 ,  C12N9/24 ,  A61K39/00

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C07K2319/01 ,  C07K2319/03 ,  C12N9/2402 ,  C12N2710/14143 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16171 ,  C12N2760/16222 ,  C12N2760/16223 ,  C12N2760/16251 ,  C12N2770/20022 ,  C12N2770/20023 ,  C12N2770/20051 ,  C12N2800/22 ,  C12Y302/01018

Abstract: This invention discloses a method of increasing production of virus-like particles comprising expressing an avian influenza matrix protein. The invention also comprises methods of making and using said VLPs.

Abstract translation: 本发明公开了一种增加包含表达禽流感基因蛋白质的病毒样颗粒的生产的方法。 本发明还包括制备和使用所述VLP的方法。