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1.发明授权Measurement of bacterial CO.sub.2 production in an isolated fluorophore by monitoring an absorbance regulated change of fluorescence 失效通过监测荧光的吸光度调节变化来测量分离的荧光团中的细菌CO 2产生
公开(公告)号：US5372784A
公开(公告)日：1994-12-13
申请号：US212674
申请日：1994-03-11
CPC分类号： G01N21/80 , C12Q1/06 , G01N21/17 , G01N21/272 , G01N21/78 , G01N21/534 , G01N2201/122 , Y10S435/808
摘要： A multi-layer blood culture sensor includes two matrices. The first matrix is a polymer that is permeable to carbon dioxide and water, but impermeable to protons. A pH sensitive absorbance based dye is encapsulated or isolated in the polymer. The second matrix is a polymer with a pH insensitive fluorescent dye encapsulated or isolated in the polymer. The matrices are spectrally coupled and are useful for the determination of microorganisms in a blood culture bottle.
摘要翻译： 多层血培养传感器包括两个基质。 第一种基质是可以渗透二氧化碳和水的聚合物，但对质子是不可渗透的。 基于pH敏感的吸光度的染料被包封或分离在聚合物中。 第二种基质是在聚合物中包封或分离的具有pH不敏感的荧光染料的聚合物。 这些基质是光谱偶联的，并且可用于测定血液培养瓶中的微生物。
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公开(公告)号：US5173434A
公开(公告)日：1992-12-22
申请号：US609278
申请日：1990-11-05
CPC分类号： G01N21/17 , G01N21/78 , G01N21/534 , G01N2201/122 , Y10S436/80
摘要： A process to determine the concentration of any substance in a colorimetric, turbidimetric or nephelometric reaction using a fluorometric detector to measure fluorescence intensity. In particular, a change in color can be monitored by observing the measurement of fluorescence intensity of a fluorophore in an inert matrix. The absorption spectrum of the chromophore may overlap the excitation and/or emission spectrum of the fluorophore, thereby allowing the change in fluorescence to be related to the intensity of color in the reaction and thus related to the quantity of the substance of interest.
摘要翻译： 使用荧光检测器测定比色，比浊或比浊法反应中任何物质的浓度以测量荧光强度的方法。 特别地，可以通过观察惰性基质中荧光团的荧光强度的测量来监测颜色变化。 发色团的吸收光谱可能与荧光团的激发和/或发射光谱重叠，从而允许荧光的变化与反应中的颜色强度相关，并且因此与感兴趣的物质的量相关。
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