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公开(公告)号：US20080194511A1
公开(公告)日：2008-08-14
申请号：US11941876
申请日：2007-11-16
IPC分类号： A61K31/711
CPC分类号： C12N15/67 , A61K48/00 , C07K14/60 , C12N15/85 , C12N2830/008 , C12N2830/15 , C12N2840/20 , C12N2840/203
摘要： One aspect of the current invention is an optimized synthetic mammalian expression plasmid (e.g. pAV0201). This new plasmid comprise a therapeutic element, and a replication element. The therapeutic element of the new plasmid comprises a eukaryotic promoter; a 5′ untranslated region (“UTR”); a codon-optimized-eukaryotic therapeutic gene sequence; and a poly adenylation signal. The therapeutic elements of this plasmid are operatively linked and located in a first operatively-linked arrangement. Additionally, the optimized synthetic mammalian expression plasmid comprises replication elements, wherein the replication elements are operatively linked and located in a second operatively-linked arrangement. The replication elements comprise a selectable marker gene promoter, a ribosomal binding site, and an origin of replication. The first-operatively-linked arrangement and the second-operatively-linked arrangement comprise a circular structure of the codon optimized synthetic mammalian expression plasmid.
摘要翻译： 本发明的一个方面是优化的合成哺乳动物表达质粒（例如pAV0201）。 该新质粒包含治疗元件和复制元件。 新质粒的治疗元件包含真核启动子; 5'非翻译区（“UTR”）; 密码子优化的真核治疗基因序列; 和聚腺苷酸化信号。 该质粒的治疗元件可操作地连接并位于第一可操作连接的布置中。 此外，优化的合成哺乳动物表达质粒包含复制元件，其中复制元件可操作地连接并位于第二可操作连接的布置中。 复制元件包括选择性标记基因启动子，核糖体结合位点和复制起点。 第一操作连接的排列和第二操作连接的排列包括密码子优化的合成哺乳动物表达质粒的圆形结构。
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公开(公告)号：US07316925B2
公开(公告)日：2008-01-08
申请号：US10619939
申请日：2003-07-15
CPC分类号： C12N15/67 , A61K48/00 , C07K14/60 , C12N15/85 , C12N2830/008 , C12N2830/15 , C12N2840/20 , C12N2840/203
摘要： One aspect of the current invention is an optimized synthetic mammalian expression plasmid (e.g. pAV0201). This new plasmid comprise a therapeutic element, and a replication element. The therapeutic element of the new plasmid comprises a eukaryotic promoter; a 5′ untranslated region (“UTR”); a codon-optimized-eukaryotic therapeutic gene sequence; and a poly adenylation signal. The therapeutic elements of this plasmid are operatively linked and located in a first operatively-linked arrangement. Additionally, the optimized synthetic mammalian expression plasmid comprises replication elements, wherein the replication elements are operatively linked and located in a second operatively-linked arrangement. The replication elements comprise a selectable marker gene promoter, a ribosomal binding site, and an origin of replication. The first-operatively-linked arrangement and the second-operatively-linked arrangement comprise a circular structure of the codon optimized synthetic mammalian expression plasmid.
摘要翻译： 本发明的一个方面是优化的合成哺乳动物表达质粒（例如pAV0201）。 该新质粒包含治疗元件和复制元件。 新质粒的治疗元件包含真核启动子; 5'非翻译区（“UTR”）; 密码子优化的真核治疗基因序列; 和聚腺苷酸化信号。 该质粒的治疗元件可操作地连接并位于第一可操作连接的布置中。 此外，优化的合成哺乳动物表达质粒包含复制元件，其中复制元件可操作地连接并位于第二可操作连接的布置中。 复制元件包括选择性标记基因启动子，核糖体结合位点和复制起点。 第一操作连接的排列和第二操作连接的排列包括密码子优化的合成哺乳动物表达质粒的圆形结构。
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3.发明授权公开(公告)号：US07579326B2
公开(公告)日：2009-08-25
申请号：US10400053
申请日：2003-03-25
申请人： Ronald V Abruzzese , Vidya Mehta , Jeffrey L Nordstrom , Jason Fewell , Bert O'Malley , Sophia Tsai
发明人： Ronald V Abruzzese , Vidya Mehta , Jeffrey L Nordstrom , Jason Fewell , Bert O'Malley , Sophia Tsai
IPC分类号： A01N43/04 , A61K31/70 , A01N63/00 , A01N65/00 , C12N15/00 , C07H19/00 , C07H21/00 , C07H21/04
CPC分类号： C12N15/63 , A61K38/1816 , A61K48/00 , A61K48/0066 , C12N15/85 , C12N2830/002 , C12N2830/008 , C12N2830/42 , C12N2830/55 , C12N2830/702 , C12N2830/85 , C12N2830/90 , C12N2840/20 , C12N2840/44
摘要： Disclosed is a novel inducible expression system characterized by undetectable biological effect in the absence of an inducer, but which exhibits efficient inducibility in the presence of an inducer.
摘要翻译： 公开了一种新颖的诱导型表达系统，其特征在于在不存在诱导物的情况下不可检测的生物学效应，但在诱导物存在下表现出有效的诱导性。
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