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公开(公告)号：US08129188B2
公开(公告)日：2012-03-06
申请号：US12155785
申请日：2008-06-10
申请人： Ming-Cheng Shih , Chin-Fu Chen , Rung-Jiun Gau , Yu-Shih Weng , Ya-Jen Yu , Shao-Jen Yeh
发明人： Ming-Cheng Shih , Chin-Fu Chen , Rung-Jiun Gau , Yu-Shih Weng , Ya-Jen Yu , Shao-Jen Yeh
CPC分类号： C12M25/14
摘要： A cell culture apparatus and a method for fabricating the cell culture apparatus are disclosed, the method comprises forming at least one fillister on a biomaterial composite layer by photolithography, wherein the biomaterial composite layer contains two gel materials. One is a bio-compatible hydrogel composition having various weight ratio of: 2-hydroxyethylmathacrylate (HEMA), bisphenol A and glycidyl methacrylate (bis-GMA), triethylene glycol dimethacrylate (TEGDMA), r-methacryloxypropyl trimethoxysilane (MAPTMS), α,α-diethoxyacetophenone (DEAP), and the other one is a photo-sensitive silica gel composition.
摘要翻译： 公开了一种细胞培养装置和细胞培养装置的制造方法，该方法包括通过光刻法在生物材料复合层上形成至少一个填充物，其中生物材料复合层含有两种凝胶材料。 一种是具有以下重要比例的生物相容性水凝胶组合物：2-羟乙基甲基丙烯酸酯（HEMA），双酚A和甲基丙烯酸缩水甘油酯（双-GMA），三乙二醇二甲基丙烯酸酯（TEGDMA），γ-甲基丙烯酰氧基丙基三甲氧基硅烷（MAPTMS），α，α 二乙氧基苯乙酮（DEAP），另一种是光敏硅胶组合物。
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公开(公告)号：US07749761B2
公开(公告)日：2010-07-06
申请号：US11446207
申请日：2006-06-05
申请人： Ming-Cheng Shih , Chin-Fu Chen , Rung-Jiun Gau , Yu-Shih Weng , Ya-Jen Yu , Shao-Jen Yeh
发明人： Ming-Cheng Shih , Chin-Fu Chen , Rung-Jiun Gau , Yu-Shih Weng , Ya-Jen Yu , Shao-Jen Yeh
CPC分类号： C12M25/14
摘要： A cell culture apparatus and a method for fabricating the cell culture apparatus are disclosed, the method comprises forming at least one fillister on a biomaterial composite layer by photolithography, wherein the biomaterial composite layer contains two gel materials. One is a bio-compatible hydrogel composition having various weight ratio of: 2-hydroxyethylmathacrylate (HEMA), bisphenolA and glycidyl methacrylate (bis-GMA), triethylene glycol dimethacrylate (TEGDMA), r-methacryloxypropyl trimethoxysilane (MAPTMS), α,α-diethoxyacetophenone (DEAP), and the other one is a photo-sensitive silica gel composition.
摘要翻译： 公开了一种细胞培养装置和细胞培养装置的制造方法，该方法包括通过光刻法在生物材料复合层上形成至少一个填充物，其中生物材料复合层含有两种凝胶材料。 一种是具有以下重量比的生物相容性水凝胶组合物：2-羟乙基甲基丙烯酸酯（HEMA），双酚A和甲基丙烯酸缩水甘油酯（双-GMA），三乙二醇二甲基丙烯酸酯（TEGDMA），γ-甲基丙烯酰氧基丙基三甲氧基硅烷（MAPTMS），α， 二乙氧基苯乙酮（DEAP），另一种是光敏硅胶组合物。
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公开(公告)号：US20070072288A1
公开(公告)日：2007-03-29
申请号：US11446207
申请日：2006-06-05
申请人： Ming-Cheng Shih , Yu-Shih Weng , Chin-Fu Chen , Rung-Jiun Gau , Ya-Jen Yu , Shao-Jen Yeh
发明人： Ming-Cheng Shih , Yu-Shih Weng , Chin-Fu Chen , Rung-Jiun Gau , Ya-Jen Yu , Shao-Jen Yeh
IPC分类号： C12M3/00
CPC分类号： C12M25/14
摘要： A cell culture apparatus and a method for fabricating the cell culture apparatus are disclosed, the method comprises forming at least one fillister on a biomaterial composite layer by photolithography, wherein the biomaterial composite layer contains two gel materials. One is a bio-compatible hydrogel composition having various weight ratio of:2-hydroxyethylmathacrylate(HEMA),bisphenolA and glycidyl methacrylate (bis-GMA), triethylene glycol dimethacrylate (TEGDMA), r-methacryloxypropyl trimethoxysilane (MAPTMS), α,α-diethoxyacetophenone (DEAP), and the other one is a photo-sensitive silica gel composition.
摘要翻译： 公开了一种细胞培养装置和细胞培养装置的制造方法，该方法包括通过光刻法在生物材料复合层上形成至少一个填充物，其中生物材料复合层含有两种凝胶材料。 一种是具有以下重量比的生物相容性水凝胶组合物：2-羟乙基甲基丙烯酸酯（HEMA），双酚A和甲基丙烯酸缩水甘油酯（双-GMA），三乙二醇二甲基丙烯酸酯（TEGDMA），γ-甲基丙烯酰氧基丙基三甲氧基硅烷（MAPTMS），α， 二乙氧基苯乙酮（DEAP），另一种是光敏硅胶组合物。
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4.发明申请Cell culture and method for screening for a compound useful in the treatment or prevention of hepatic cirrhosis 有权用于筛选用于治疗或预防肝硬化的化合物的细胞培养和方法公开(公告)号：US20060172416A1
公开(公告)日：2006-08-03
申请号：US11298586
申请日：2005-12-12
申请人： Wen-Tyng Li , Rung-Jiun Gau , Yu-Shih Weng , Cheng-Ta Hsieh , Pei-Shan Li
发明人： Wen-Tyng Li , Rung-Jiun Gau , Yu-Shih Weng , Cheng-Ta Hsieh , Pei-Shan Li
IPC分类号： C12N5/08
CPC分类号： G01N33/5067 , G01N33/5023 , G01N2800/085
摘要： The present invention provides a cell-culturing model and a method for screening compounds which can be applied in treating or preventing hepatic cirrhosis. The cell culturing model comprises a population of hepatocytes and hepatic stellate cells (HSCs) derived from co-culturing, and at least one population of the cells comprises a nucleotide sequence fragment of a reporter gene and a cell specific regulatory sequence. The cell-culturing model of the present invention can be applied in high throughput screening for effective compounds of medication, and also in understanding the functional mechanism of the effective compounds.
摘要翻译： 本发明提供了可用于治疗或预防肝硬化的化合物的细胞培养模型和筛选方法。 细胞培养模型包括源自共培养的肝细胞群和肝星状细胞（HSC），至少一个细胞群包含报告基因的核苷酸序列片段和细胞特异性调节序列。 本发明的细胞培养模型可以用于高通量筛选有效的药物化合物，也可用于理解有效化合物的功能机制。
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公开(公告)号：US20070122897A1
公开(公告)日：2007-05-31
申请号：US11503123
申请日：2006-08-14
申请人： Yu-Shi Weng , Ming-Cheng Shih , Rung-Jiun Gau
发明人： Yu-Shi Weng , Ming-Cheng Shih , Rung-Jiun Gau
摘要： A micro device for cell culture is disclosed, which cooperates with a fluid and includes: a top plate having an inlet port; a orifice plate having a plurality of orifices; a culture plate having a plurality of culture wells and a plurality of injection ports; and a bottom plate having at least one collecting well and at least one collecting flow channel, wherein, the culture plate is placed between the orifice plate and the bottom plate. The collecting flow channel connects to all regulating orifices in the culture wells and guides the fluid from the culture wells, then receives the fluid in the collecting well. The fluid flows into the orifice plate from the inlet port of the top plate, and then diversifies into the culture plate, then arrives at each culture well by way of the injection ports, and finally collects in the collecting well of the bottom plate.
摘要翻译： 公开了一种用于细胞培养的微型装置，其与流体协作并且包括：具有入口的顶板; 具有多个孔口的孔板; 具有多个培养孔和多个注射口的培养板; 以及具有至少一个收集井和至少一个收集流动通道的底板，其中，所述培养板被放置在所述孔板和所述底板之间。 收集流通道连接到培养井中的所有调节孔，并引导来自培养井的流体，然后在收集井中接收流体。 流体从顶板的入口流入孔板，然后分散到培养板中，然后通过注射口到达每个培养井，最后收集在底板的收集井中。
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公开(公告)号：US07820430B2
公开(公告)日：2010-10-26
申请号：US11503123
申请日：2006-08-14
申请人： Yu-Shi Weng , Ming-Cheng Shih , Rung-Jiun Gau
发明人： Yu-Shi Weng , Ming-Cheng Shih , Rung-Jiun Gau
IPC分类号： C12M3/00
摘要： A micro device for cell culture is disclosed, which cooperates with a fluid and includes: a top plate having an inlet port; a orifice plate having a plurality of orifices; a culture plate having a plurality of culture wells and a plurality of injection ports; and a bottom plate having at least one collecting well and at least one collecting flow channel, wherein, the culture plate is placed between the orifice plate and the bottom plate. The collecting flow channel connects to all regulating orifices in the culture wells and guides the fluid from the culture wells, then receives the fluid in the collecting well. The fluid flows into the orifice plate from the inlet port of the top plate, and then diversifies into the culture plate, then arrives at each culture well by way of the injection ports, and finally collects in the collecting well of the bottom plate.
摘要翻译： 公开了一种用于细胞培养的微型装置，其与流体协作并且包括：顶板，其具有入口; 具有多个孔口的孔板; 具有多个培养孔和多个注射口的培养板; 以及具有至少一个收集井和至少一个收集流动通道的底板，其中，所述培养板被放置在所述孔板和所述底板之间。 收集流通道连接到培养井中的所有调节孔，并引导来自培养井的流体，然后在收集井中接收流体。 流体从顶板的入口流入孔板，然后分散到培养板中，然后通过注射口到达每个培养井，最后收集在底板的收集井中。
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公开(公告)号：US20090004731A1
公开(公告)日：2009-01-01
申请号：US12155785
申请日：2008-06-10
申请人： Ming-Cheng Shih , Chin-Fu Chen , Rung-Jiun Gau , Yu-Shih Weng , Ya-Jen Yu , Shao-Jen Yeh
发明人： Ming-Cheng Shih , Chin-Fu Chen , Rung-Jiun Gau , Yu-Shih Weng , Ya-Jen Yu , Shao-Jen Yeh
CPC分类号： C12M25/14
摘要： A cell culture apparatus and a method for fabricating the cell culture apparatus are disclosed, the method comprises forming at least one fillister on a biomaterial composite layer by photolithography, wherein the biomaterial composite layer contains two gel materials. One is a bio-compatible hydrogel composition having various weight ratio of: 2-hydroxyethylmathacrylate (HEMA), bisphenol A and glycidyl methacrylate (bis-GMA), triethylene glycol dimethacrylate (TEGDMA), r-methacryloxypropyl trimethoxysilane (MAPTMS), α,α-diethoxyacetophenone (DEAP), and the other one is a photo-sensitive silica gel composition.
摘要翻译： 公开了一种细胞培养装置和细胞培养装置的制造方法，该方法包括通过光刻法在生物材料复合层上形成至少一个填充物，其中生物材料复合层含有两种凝胶材料。 一种是具有各种重量比的生物相容性水凝胶组合物：2-羟乙基甲基丙烯酸酯（HEMA），双酚A和甲基丙烯酸缩水甘油酯（双-GMA），三乙二醇二甲基丙烯酸酯（TEGDMA），γ-甲基丙烯酰氧基丙基三甲氧基硅烷（MAPTMS），α，α 二乙氧基苯乙酮（DEAP），另一种是光敏硅胶组合物。
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8.发明授权Cell culture and method for screening for a compound useful in the treatment or prevention of hepatic cirrhosis 有权用于筛选用于治疗或预防肝硬化的化合物的细胞培养和方法公开(公告)号：US07745120B2
公开(公告)日：2010-06-29
申请号：US11298586
申请日：2005-12-12
申请人： Wen-Tyng Li , Rung-Jiun Gau , Yu-Shih Weng , Cheng-Ta Hsieh , Pei-Shan Li
发明人： Wen-Tyng Li , Rung-Jiun Gau , Yu-Shih Weng , Cheng-Ta Hsieh , Pei-Shan Li
CPC分类号： G01N33/5067 , G01N33/5023 , G01N2800/085
摘要： The present invention provides a cell-culturing model and a method for screening compounds which can be applied in treating or preventing hepatic cirrhosis. The cell culturing model comprises a population of hepatocytes and hepatic stellate cells (HSCs) derived from co-culturing, and at least one population of the cells comprises a nucleotide sequence fragment of a reporter gene and a cell specific regulatory sequence. The cell-culturing model of the present invention can be applied in high throughput screening for effective compounds of medication, and also in understanding the functional mechanism of the effective compounds.
摘要翻译： 本发明提供了可用于治疗或预防肝硬化的化合物的细胞培养模型和筛选方法。 细胞培养模型包括源自共培养的肝细胞群和肝星状细胞（HSC），至少一个细胞群包含报告基因的核苷酸序列片段和细胞特异性调节序列。 本发明的细胞培养模型可以用于高通量筛选有效的药物化合物，也可用于理解有效化合物的功能机制。
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