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公开(公告)号：US20130203153A1
公开(公告)日：2013-08-08
申请号：US12983922
申请日：2011-01-04
IPC分类号： C12N15/74
CPC分类号： C12N15/74 , C07K14/195 , C12N15/52 , C12P19/04
摘要： The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene 1 Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
摘要翻译： 本发明提供由突变基因1鞘氨醇单胞菌菌株产生的改善的多糖（例如，结冷糖和二萜），其含有至少一种有利于形成泥质多糖的生物的遗传修饰。 还提供了制备突变型鞘氨醇单胞菌菌株和含有这种突变型鞘氨醇单胞菌属菌株的培养液的方法。
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2.发明申请Method for Rapidly Screening Microbial Hosts to Identify Certain Strains with Improved Yield and/or Quality in the Expression of Heterologous Proteins 有权快速筛选微生物宿主鉴定异源蛋白表达中产量和/或质量提高的某些菌株的方法公开(公告)号：US20100137162A1
公开(公告)日：2010-06-03
申请号：US12610207
申请日：2009-10-30
申请人： Diane M. Retallack , Charles H. Squires , Thomas M. Ramseier , Russell J. Coleman , Jane C. Schneider , Charies D. Hershberger
发明人： Diane M. Retallack , Charles H. Squires , Thomas M. Ramseier , Russell J. Coleman , Jane C. Schneider , Charies D. Hershberger
CPC分类号： C12N15/1037 , C12N15/78
摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.
摘要翻译： 本发明提供了一种用于快速鉴定能够产生具有改善的产量和/或质量的异源蛋白质的宿主细胞群的阵列。 该阵列包含已被遗传修饰的一个或多个宿主细胞群，以增加涉及蛋白质产生的一个或多个靶基因的表达，减少参与蛋白质降解的一个或多个靶基因的表达，或两者。 阵列中的一个或多个菌株可以在周质隔室中表达感兴趣的异源蛋白质，或者通过外细胞壁在细胞外分泌异源蛋白质。 菌株阵列可用于筛选任何目的蛋白质的表达，包括治疗性蛋白质，激素，生长因子，细胞外受体或配体，蛋白酶，激酶，血液蛋白，趋化因子，细胞因子，抗体等。
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3.发明申请METHOD FOR RAPIDLY SCREENING MICROBIAL HOSTS TO IDENTIFY CERTAIN STRAINS WITH IMPROVED YIELD AND/OR QUALITY IN THE EXPRESSION OF HETEROLOGOUS PROTEINS 有权用于快速筛选微生物宿主以鉴别异源蛋白质表达中具有改善的产量和/或质量的某些菌株的方法公开(公告)号：US20080269070A1
公开(公告)日：2008-10-30
申请号：US12109554
申请日：2008-04-25
摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.
摘要翻译： 本发明提供了一种用于快速鉴定能够产生具有改善的产量和/或质量的异源蛋白质的宿主细胞群的阵列。 该阵列包含已被遗传修饰的一个或多个宿主细胞群，以增加涉及蛋白质产生的一个或多个靶基因的表达，减少参与蛋白质降解的一个或多个靶基因的表达，或两者。 阵列中的一个或多个菌株可以在周质隔室中表达感兴趣的异源蛋白质，或者通过外细胞壁在细胞外分泌异源蛋白质。 菌株阵列可用于筛选任何目的蛋白质的表达，包括治疗性蛋白质，激素，生长因子，细胞外受体或配体，蛋白酶，激酶，血液蛋白，趋化因子，细胞因子，抗体等。
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公开(公告)号：US08759071B2
公开(公告)日：2014-06-24
申请号：US12983922
申请日：2011-01-04
IPC分类号： C12N15/74
CPC分类号： C12N15/74 , C07K14/195 , C12N15/52 , C12P19/04
摘要： The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene I Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
摘要翻译： 本发明提供了由含有至少一种有利于形成泥质多糖生产的遗传修饰的突变基因I鞘氨醇单胞菌菌株产生的改良多糖（例如，结冷糖和二萜）。 还提供了制备突变型鞘氨醇单胞菌菌株和含有这种突变型鞘氨醇单胞菌属菌株的培养液的方法。
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公开(公告)号：US07618799B2
公开(公告)日：2009-11-17
申请号：US12022789
申请日：2008-01-30
申请人： Russell J. Coleman , Diane Retallack , Jane C. Schneider , Thomas M. Ramseier , Charles D. Hershberger , Stacey Lee , Sol M. Resnick
发明人： Russell J. Coleman , Diane Retallack , Jane C. Schneider , Thomas M. Ramseier , Charles D. Hershberger , Stacey Lee , Sol M. Resnick
CPC分类号： C12N15/625 , C07K2319/02 , C07K2319/036
摘要： Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.
摘要翻译： 提供用于改善宿主细胞中感兴趣的蛋白质或多肽的表达和/或分泌的组合物和方法。 提供了包含细菌分泌信号肽的编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达感兴趣的蛋白质或多肽。 本发明的组合物可用于增加正确加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加来自宿主细胞的适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示氨基酸序列的核苷酸序列的分离的核酸分子。 ，以及SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。
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公开(公告)号：US08716003B2
公开(公告)日：2014-05-06
申请号：US12983914
申请日：2011-01-04
CPC分类号： C12N15/74 , C07K14/195 , C12N15/52 , C12P19/04
摘要： The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene R Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
摘要翻译： 本发明提供了由含有至少一种有利于形成多糖的产生的遗传修饰的突变基因R鞘氨醇单胞菌菌株产生的改良的多糖（例如，结冷糖和二萜）。 还提供了制备突变型鞘氨醇单胞菌菌株和含有这种突变型鞘氨醇单胞菌属菌株的培养液的方法。
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公开(公告)号：US20110171721A1
公开(公告)日：2011-07-14
申请号：US12983914
申请日：2011-01-04
CPC分类号： C12N15/74 , C07K14/195 , C12N15/52 , C12P19/04
摘要： The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene R Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
摘要翻译： 本发明提供了由含有至少一种有利于形成多糖的产生的遗传修饰的突变基因R鞘氨醇单胞菌菌株产生的改良的多糖（例如，结冷糖和二萜）。 还提供了制备突变型鞘氨醇单胞菌菌株和含有这种突变型鞘氨醇单胞菌属菌株的培养液的方法。
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公开(公告)号：US07888333B2
公开(公告)日：2011-02-15
申请号：US11347341
申请日：2006-02-03
IPC分类号： A61K31/715 , C08B37/00
CPC分类号： C12N15/74 , C07K14/195 , C12N15/52 , C12P19/04
摘要： The present invention provides improved diutan polysaccharides produced by mutant Sphingomonas strains containing at least one genetic modification that favors slime-forming diutan production that exhibits increased viscosity. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
摘要翻译： 本发明提供了由突变型鞘氨醇单胞菌属菌株产生的改进的二脱水多糖，其含有至少一种有利于显示增加的粘度的粘液形成二萜生产的遗传修饰。 还提供了制备突变型鞘氨醇单胞菌菌株和含有这种突变型鞘氨醇单胞菌属菌株的培养液的方法。
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公开(公告)号：US20110020868A1
公开(公告)日：2011-01-27
申请号：US12884095
申请日：2010-09-16
CPC分类号： C12N15/625 , C07K2319/02 , C07K2319/036
摘要： Compositions and methods for improving expression and/or secretion of a polypeptide of interest in a host cell are provided. Compositions including a coding sequence for a bacterial secretion signal peptide are provided. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. The present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.
摘要翻译： 提供用于改善宿主细胞中感兴趣的多肽的表达和/或分泌的组合物和方法。 提供了包括细菌分泌信号肽的编码序列的组合物。 本发明的组合物可用于增加适当加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 本发明提供了分离的核酸分子，包括编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示的氨基酸序列的核苷酸序列， SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。
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公开(公告)号：US20100048864A1
公开(公告)日：2010-02-25
申请号：US12604061
申请日：2009-10-22
CPC分类号： C12N15/625 , C07K2319/02 , C07K2319/036
摘要： Compositions and methods for improving expression and/or secretion of a polypeptide of interest in a host cell are provided. Compositions including a coding sequence for a bacterial secretion signal peptide are provided. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. The present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.
摘要翻译： 提供用于改善宿主细胞中感兴趣的多肽的表达和/或分泌的组合物和方法。 提供了包括细菌分泌信号肽的编码序列的组合物。 本发明的组合物可用于增加适当加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 本发明提供了分离的核酸分子，包括编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示的氨基酸序列的核苷酸序列， SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。
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