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    Bacterial leader sequences for increased expression
    1.
    发明授权
    Bacterial leader sequences for increased expression 有权
    用于增加表达的细菌前导序列

    公开(公告)号：US07618799B2

    公开(公告)日：2009-11-17

    申请号：US12022789

    申请日：2008-01-30

    申请人： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

    发明人： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

    IPC分类号： C12N9/00 ,  C12N15/00 ,  C12N1/04

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/036

    摘要： Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

    摘要翻译： 提供用于改善宿主细胞中感兴趣的蛋白质或多肽的表达和/或分泌的组合物和方法。 提供了包含细菌分泌信号肽的编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达感兴趣的蛋白质或多肽。 本发明的组合物可用于增加正确加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加来自宿主细胞的适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示氨基酸序列的核苷酸序列的分离的核酸分子。 ，以及SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。

    Enzymatic resolution of propylene glycol alkyl (or aryl) ethers and ether acetates
    2.
    发明授权
    Enzymatic resolution of propylene glycol alkyl (or aryl) ethers and ether acetates 失效
    丙二醇烷基（或芳基）醚和醚乙酸酯的酶解

    公开(公告)号：US07264960B2

    公开(公告)日：2007-09-04

    申请号：US10502692

    申请日：2003-03-21

    申请人： Sol M. Resnick ,  Felipe A. Donate ,  Timothy C. Frank ,  Thomas C. Thyne ,  Paul Foley

    发明人： Sol M. Resnick ,  Felipe A. Donate ,  Timothy C. Frank ,  Thomas C. Thyne ,  Paul Foley

    IPC分类号： C12P41/00

    CPC分类号： C12P7/18 ,  C12P41/004

    摘要： Glycol ether acetates, and in particular propylene glycol alkyl (or aryl) ether acetates, can be resolved enzymatically by enantioselective hydrolysis with a hydrolase at high concentrations of substrates; in some embodiments, the hydrolase is a lipase. Glycol ethers, and in particular propylene glycol alkyl (or aryl) ethers, can be resolved enzymatically by enantioselective transesterification with a hydrolase, in the presence of an acyl donor, at high concentrations of substrates; in some embodiments, the hydrolase is a lipase.

    摘要翻译： 乙二醇醚乙酸酯，特别是丙二醇烷基（或芳基）醚乙酸酯可以通过在高浓度底物下用水解酶进行对映选择性水解来酶解; 在一些实施方案中，水解酶是脂肪酶。 乙二醇醚，特别是丙二醇烷基（或芳基）醚可以通过在酰基供体存在下，在高浓度底物下用水解酶进行对映选择性酯交换酶解解; 在一些实施方案中，水解酶是脂肪酶。

    BACTERIAL LEADER SEQUENCES FOR INCREASED EXPRESSION
    3.
    发明申请
    BACTERIAL LEADER SEQUENCES FOR INCREASED EXPRESSION 有权
    细菌引导序列增加表达

    公开(公告)号：US20080193974A1

    公开(公告)日：2008-08-14

    申请号：US12022789

    申请日：2008-01-30

    申请人： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

    发明人： Russell J. Coleman ,  Diane Retallack ,  Jane C. Schneider ,  Thomas M. Ramseier ,  Charles D. Hershberger ,  Stacey Lee ,  Sol M. Resnick

    IPC分类号： C12P21/04 ,  C12N15/11 ,  C12N15/00 ,  C12N1/20 ,  C07K14/00 ,  C12N9/90

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/036

    摘要： Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

    摘要翻译： 提供用于改善宿主细胞中感兴趣的蛋白质或多肽的表达和/或分泌的组合物和方法。 提供了包含细菌分泌信号肽的编码序列的组合物。 编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达感兴趣的蛋白质或多肽。 本发明的组合物可用于增加正确加工的蛋白质在宿主细胞的周质空间中的积累，或用于增加来自宿主细胞的适当加工的蛋白质的分泌。 特别地，提供了分离信号肽编码核酸分子。 另外，包含对应于核酸分子的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4,6,8,10,12,14,16,18,20,22和24所示氨基酸序列的核苷酸序列的分离的核酸分子。 ，以及SEQ ID NO：1,3,5,7,9,11,13,15,17,19,21和23所示的核苷酸序列，以及其变体和片段。