公开(公告)号：US20150361446A1

公开(公告)日：2015-12-17

申请号：US14763239

申请日：2014-01-23

申请人： Mary BEATTY ,  Kent BRINK ,  Virginia CRANE ,  Scott DIEHN ,  Albert L. LU ,  Gregory J. YOUNG ,  PIONEER HI-BRED INTERNATIONAL, INC. ,  E. I. DUPONT DE NEMOURS & COMPANY

发明人： Mary Beatty ,  Kent BRINK ,  Virginia CRANE ,  Scott DIEHN ,  Albert L. LU ,  Gregory J. YOUNG

IPC分类号： C12N15/82 ,  C12Q1/68 ,  A01H5/10

CPC分类号： C12N15/8286 ,  A01H5/10 ,  C07K14/325 ,  C12N15/8277 ,  C12Q1/6895 ,  C12Q2600/13 ,  Y02A40/162

摘要： The disclosure provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-033121-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

摘要翻译： 本公开提供与转基因抗虫玉米植物相关的DNA组合物。 还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-033121-3事件的存在的测定法。 提供了用于进行测定的试剂盒和条件。

公开(公告)号：US20130145502A1

公开(公告)日：2013-06-06

申请号：US13701848

申请日：2011-06-09

申请人： Scott Diehn ,  Ajit Nott ,  David A. Selinger ,  Carl R. Simmons ,  Priyanka Bhyri ,  Venkata S. Tavva

发明人： Scott Diehn ,  Ajit Nott ,  David A. Selinger ,  Carl R. Simmons ,  Priyanka Bhyri ,  Venkata S. Tavva

IPC分类号： C12N15/82

CPC分类号： C12N15/8216

摘要： The invention relates to gene expression regulatory sequences, specifically introns that act as enhancers of gene expression, the promoter and terminator sequences endogenously associated with these introns. Presence of these intronic enhancer sequences in proximity to promoter sequences leads to enhancement of gene expression. Methods of finding such new intronic enhancer sequences and using them to generate transgenic plants are also described.

公开(公告)号：US20110209242A1

公开(公告)日：2011-08-25

申请号：US13028367

申请日：2011-02-16

申请人： Scott Diehn ,  Albert L. Lu ,  Lynne E. Sims

发明人： Scott Diehn ,  Albert L. Lu ,  Lynne E. Sims

IPC分类号： C12N15/82 ,  C07H21/00 ,  C12N15/63 ,  C12N5/10 ,  A01H5/00 ,  A01H5/10

CPC分类号： C07K14/415 ,  C12N15/8223 ,  C12N15/8225

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a green tissue-preferred promoter from maize. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于来自玉米的绿色组织优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US07411112B2

公开(公告)日：2008-08-12

申请号：US10961629

申请日：2004-10-08

申请人： Scott Diehn ,  Albert Laurence Lu ,  Steven W. Ritchie ,  Lynne Sims ,  Kim R. Ward ,  Philip Benfey ,  Jee W. Jung

发明人： Scott Diehn ,  Albert Laurence Lu ,  Steven W. Ritchie ,  Lynne Sims ,  Kim R. Ward ,  Philip Benfey ,  Jee W. Jung

IPC分类号： C12N15/82 ,  A01H5/00 ,  C07H21/04

CPC分类号： C12N15/8227

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding CRWAQ81. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码CRWAQ81的基因的根优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US07214854B2

公开(公告)日：2007-05-08

申请号：US11022111

申请日：2004-12-22

申请人： Scott Diehn ,  Albert L. Lu

发明人： Scott Diehn ,  Albert L. Lu

IPC分类号： C12N15/82 ,  A01H5/00 ,  C07H21/04

CPC分类号： C12N15/8227 ,  C12N15/8222

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a maize metallothionein promoter for the gene encoding metallothionein. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码金属硫蛋白的基因的玉米金属硫蛋白启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括用与本发明的启动子之一可操作地连接的核苷酸序列转化植物或植物细胞。

公开(公告)号：US07193135B2

公开(公告)日：2007-03-20

申请号：US11299452

申请日：2005-12-12

申请人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

发明人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

IPC分类号： C12P7/62 ,  C12P11/00 ,  C07H21/04 ,  A01H1/00

CPC分类号： C12N9/0069 ,  C07K14/415 ,  C12N15/8227 ,  C12N15/8237 ,  C12N15/8239

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize 2-oxoglutarate-dependent oxygenase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码玉米2-氧戊二酸依赖性加氧酶的基因的诱导型启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US20050097633A1

公开(公告)日：2005-05-05

申请号：US10961629

申请日：2004-10-08

申请人： Scott Diehn ,  Albert Lu ,  Steven Ritchie ,  Lynne Sims ,  Kim Ward ,  Philip Benfey ,  Jee Jung

发明人： Scott Diehn ,  Albert Lu ,  Steven Ritchie ,  Lynne Sims ,  Kim Ward ,  Philip Benfey ,  Jee Jung

IPC分类号： C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C07K14/415 ,  C12N5/04

CPC分类号： C12N15/8227

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding CRWAQ81. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码CRWAQ81的基因的根优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US08962916B2

公开(公告)日：2015-02-24

申请号：US13329511

申请日：2011-12-19

申请人： Scott Diehn ,  Albert L. Lu ,  Carl R. Simmons

发明人： Scott Diehn ,  Albert L. Lu ,  Carl R. Simmons

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N15/00 ,  C12N5/04 ,  C12N5/14 ,  C07H21/04

CPC分类号： C12N15/8226 ,  C12N15/8216 ,  C12N15/8227 ,  C12N15/8286 ,  Y02A40/162

摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将与本发明的启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US20140275208A1

公开(公告)日：2014-09-18

申请号：US13831230

申请日：2013-03-14

申请人： XU HU ,  JAMES PRESNAIL ,  NINA Richtman ,  SCOTT DIEHN ,  MICHELLE HARNACK ,  LISA PROCYK

发明人： XU HU ,  JAMES PRESNAIL ,  NINA Richtman ,  SCOTT DIEHN ,  MICHELLE HARNACK ,  LISA PROCYK

IPC分类号： A01N57/16 ,  C12N15/113

CPC分类号： C12N15/113 ,  A01N37/46 ,  A01N63/02 ,  C12N15/8218 ,  C12N15/8286 ,  C12N2310/14 ,  C12N2310/531 ,  Y02A40/162

摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiments, the pest is D. virgifera virgifera, D. barberi, D. virgifera zeae, D. speciosa, D. speciosa, or D. undecimpunctata howardi. Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

摘要翻译： 提供了使用沉积元件的方法和组合物，当由有害生物摄取的诸如鞘翅目植物病虫害（Diaprotica plant pest）的害虫时，会降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供了本文所公开的任何一个（但不包括正向和反向引物）或其活性变体及其片段或其互补序列的任何一种或多种靶基因，其中一个或多个 目标害虫中更多的序列控制害虫（即具有杀虫活性）。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，害虫是D. virgifera virgifera，D.barberi，D. virgifera zeae，D. speciosa，D. speciosa或D. undecimpunctata howardi。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

公开(公告)号：US08395022B2

公开(公告)日：2013-03-12

申请号：US12847534

申请日：2010-07-30

申请人： Scott Diehn ,  Albert L. Lu ,  Carl R. Simmons

发明人： Scott Diehn ,  Albert L. Lu ,  Carl R. Simmons

IPC分类号： C12N15/82 ,  C12N15/113 ,  C12N5/10

CPC分类号： C12N15/8222

摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.