公开(公告)号：US20140024070A1

公开(公告)日：2014-01-23

申请号：US14003541

申请日：2012-03-06

申请人： Saki Izumi ,  Takafumi Komori ,  Yoshitane Nozaki

发明人： Saki Izumi ,  Takafumi Komori ,  Yoshitane Nozaki

IPC分类号： G01N21/64

CPC分类号： G01N21/6486 ,  C09B11/24 ,  G01N33/5008 ,  G01N33/5067 ,  G01N33/582 ,  G01N33/6872 ,  G01N2500/00

摘要： The present invention provides a method of screening for a compound that enhances or inhibits the transport activity of OATP1B1, using dichlorofluorescein. The present invention also provides a use of dichlorofluorescein in measurement of the expression level of OATP1B1. The present invention further provides a method for measuring the expression level of OATP1B1 in test cells, using dichlorofluorescein. The present invention further provides a use of a kit including dichlorofluorescein and positive cells expressing OATP1B1 in measurement of the expression level of OATP1B1 in test cells.

摘要翻译： 本发明提供了使用二氯荧光素筛选增强或抑制OATP1B1的转运活性的化合物的方法。 本发明还提供了二氯荧光素在测量OATP1B1表达水平方面的用途。 本发明还提供了使用二氯荧光素测量测试细胞中OATP1B1的表达水平的方法。 本发明还提供了包含二氯荧光素和表达OATP1B1的阳性细胞的试剂盒在测试细胞中测量OATP1B1表达水平的用途。

公开(公告)号：US08410164B2

公开(公告)日：2013-04-02

申请号：US12991750

申请日：2009-05-15

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

IPC分类号： A61K31/352 ,  C07D311/82

CPC分类号： G01N31/225 ,  C07D311/82 ,  C07D493/10 ,  G01N2021/7786

摘要： A compound represented by the formula (I) (R1 represents an aryl group such as hydroxyphenyl group, R2 represents 2-carboxyphenyl group etc., and R3 and R4 represent —(CH2)p—N(R5)(R6) (R5 and R6 represent —(CH2)n—COOH), a salt thereof, or an ester thereof, which is useful as a reagent for highly sensitive measurement of reactive oxygen such as hydroxy radical present at a trace amount in living bodies or tissues by a bioimaging technique over a long period of time.

摘要翻译： 由式（I）表示的化合物（R 1表示芳基如羟基苯基，R 2表示2-羧基苯基等，R 3和R 4表示 - （CH 2）p -N（R 5）（R 6）（R 5和 R6表示 - （CH2）n-COOH），其盐或其酯，可用作活性氧如活体或组织中微量存在的活性氧的高灵敏度测定的试剂，通过生物成像 技术在很长一段时间内。

公开(公告)号：US20110287552A1

公开(公告)日：2011-11-24

申请号：US13131173

申请日：2009-12-04

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

IPC分类号： G01N21/76 ,  C07D493/10

CPC分类号： G01N31/22 ,  Y10T436/170769 ,  Y10T436/173076 ,  Y10T436/177692 ,  Y10T436/178459 ,  Y10T436/179228

摘要： A compound represented by the general formula (I) [R1 and R2 are amino groups that substitute at adjacent positions on the benzene ring; R3 and R4 are halogen atoms; R5 and R6 represent hydrogen atom, an acyl group or an acyloxy(C1-6 alkyl) group; R7 and R8 represent —(CH2)p—N(R9)(R10) (p is 1 to 4, and R9 and R10 represent —(CH2)n—COOH (n is 1 to 4))], which is useful for measuring a reactive nitrogen species existing in cells such as nitrogen monoxide or peroxynitrite at high sensitivity over a long period of time.

摘要翻译： 由通式（I）表示的化合物[R1和R2是在苯环上的相邻位置取代的氨基; R3和R4是卤素原子; R5和R6代表氢原子，酰基或酰氧基（C1-6烷基）基团; R7和R8表示 - （CH2）p-N（R9）（R10）（p为1至4，R9和R10表示 - （CH2）n-COOH（n为1至4））] 在长时间内以高灵敏度测量存在于细胞中的活性氮物质，例如一氧化氮或过氧亚硝酸盐。

公开(公告)号：US20160349658A1

公开(公告)日：2016-12-01

申请号：US15155317

申请日：2016-05-16

申请人： Saki IZUMI ,  Hiroyuki SUGIYAMA ,  Shunichi HASHIMOTO ,  Naoyuki OZAKI ,  Mikio ISHIBASHI ,  Kayoko TANAKA ,  Kohta FUJIMORI

发明人： Saki IZUMI ,  Hiroyuki SUGIYAMA ,  Shunichi HASHIMOTO ,  Naoyuki OZAKI ,  Mikio ISHIBASHI ,  Kayoko TANAKA ,  Kohta FUJIMORI

IPC分类号： G03G15/02

CPC分类号： G03G15/0266 ,  G03G15/5058

摘要： An image forming apparatus includes a latent image bearer to rotate, an image forming unit including a charger and a developing device, a charge power supply to output a charging bias applied to a charger, a transfer device, a toner adhesion amount, and a controller. The controller causes the image forming unit to form a background fog pattern in a background area of the latent image bearer while changing a background potential, acquires toner adhesion amount values detected at different positions of the background fog pattern, having different potentials, sorts the toner adhesion amount values in an order of the background potential, determines a relation between the background potential and background fog amount based the toner adhesion amount values except any toner adhesion amount value out of monotonicity, and adjust the charging bias to an optimum value computed based on the determined relation.

摘要翻译： 图像形成装置包括要转动的潜像载体，包括充电器和显影装置的图像形成单元，用于输出施加到充电器的充电偏压的充电电源，转印装置，调色剂附着量和控制器 。 控制器使图像形成单元在改变背景电位的同时在潜像承载体的背景区域中形成背景模糊图案，获取在具有不同电位的背景模式的不同位置处检测到的调色剂附着量值，对调色剂 基于背景电位的顺序的粘附量值基于除了单调性之外的任何调色剂附着量值之外的调色剂附着量值，确定背景电位和背景灰度量之间的关系，并将充电偏压调整到基于 确定的关系。

公开(公告)号：US09977361B2

公开(公告)日：2018-05-22

申请号：US15354401

申请日：2016-11-17

申请人： Hideaki Kanaya ,  Taichi Urayama ,  Ryusuke Mase ,  Hiroki Ishii ,  Hiroyuki Sugiyama ,  Saki Izumi ,  Yuushi Hirayama

发明人： Hideaki Kanaya ,  Taichi Urayama ,  Ryusuke Mase ,  Hiroki Ishii ,  Hiroyuki Sugiyama ,  Saki Izumi ,  Yuushi Hirayama

IPC分类号： G03G15/02 ,  G03G15/00 ,  G03G21/20

CPC分类号： G03G15/0266 ,  G03G15/5058 ,  G03G21/20

摘要： An image forming apparatus includes an image bearer, a charging, a charge power supply to supply a charging bias to the charging device, a developing device, a toner adhesion amount detector, an environment detector, and a controller to determine whether to execute a charging bias adjustment process in which the charging device charges the image bearer to have different potentials, the developing device supplies the toner to the image bearer according to the different potentials, the toner adhesion amount detector detects the amount of toner adhering to the image bearer, and the controller adjusts the charging. The controller includes a memory device to store the environment data, and is configured to compare the environment data with previous environment data stored in the memory device, and determine not to execute the charging bias adjustment process when an environment change amount is not greater than a threshold.

公开(公告)号：US20170153564A1

公开(公告)日：2017-06-01

申请号：US15354401

申请日：2016-11-17

申请人： Hideaki KANAYA ,  Taichi URAYAMA ,  Ryusuke MASE ,  Hiroki ISHll ,  Hiroyuki SUGIYAMA ,  Saki IZUMI ,  Yuushi HIRAYAMA

发明人： Hideaki KANAYA ,  Taichi URAYAMA ,  Ryusuke MASE ,  Hiroki ISHll ,  Hiroyuki SUGIYAMA ,  Saki IZUMI ,  Yuushi HIRAYAMA

IPC分类号： G03G15/02

CPC分类号： G03G15/0266 ,  G03G15/5058 ,  G03G21/20

摘要： An image forming apparatus includes an image bearer, a charging, a charge power supply to supply a charging bias to the charging device, a developing device, a toner adhesion amount detector, an environment detector, and a controller to determine whether to execute a charging bias adjustment process in which the charging device charges the image bearer to have different potentials, the developing device supplies the toner to the image bearer according to the different potentials, the toner adhesion amount detector detects the amount of toner adhering to the image bearer, and the controller adjusts the charging. The controller includes a memory device to store the environment data, and is configured to compare the environment data with previous environment data stored in the memory device, and determine not to execute the charging bias adjustment process when an environment change amount is not greater than a threshold.

公开(公告)号：US20110251404A1

公开(公告)日：2011-10-13

申请号：US12991750

申请日：2009-05-15

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

IPC分类号： C07D311/82 ,  C07D311/90

CPC分类号： G01N31/225 ,  C07D311/82 ,  C07D493/10 ,  G01N2021/7786

摘要： A compound represented by the formula (I) (R1 represents an aryl group such as hydroxyphenyl group, R2 represents 2-carboxyphenyl group etc., and R3 and R4 represent —(CH2)p—N(R5)(R6) (R5 and R6 represent —(CH2)n—COOH), a salt thereof, or an ester thereof, which is useful as a reagent for highly sensitive measurement of reactive oxygen such as hydroxy radical present at a trace amount in living bodies or tissues by a bioimaging technique over a long period of time.

摘要翻译： 由式（I）表示的化合物（R 1表示芳基如羟基苯基，R 2表示2-羧基苯基等，R 3和R 4表示 - （CH 2）p -N（R 5）（R 6）（R 5和 R6表示 - （CH2）n-COOH），其盐或其酯，可用作活性氧如活体或组织中微量存在的活性氧的高灵敏度测定的试剂，通过生物成像 技术在很长一段时间内。

公开(公告)号：US09606469B2

公开(公告)日：2017-03-28

申请号：US15155317

申请日：2016-05-16

申请人： Saki Izumi ,  Hiroyuki Sugiyama ,  Shunichi Hashimoto ,  Naoyuki Ozaki ,  Mikio Ishibashi ,  Kayoko Tanaka ,  Kohta Fujimori

发明人： Saki Izumi ,  Hiroyuki Sugiyama ,  Shunichi Hashimoto ,  Naoyuki Ozaki ,  Mikio Ishibashi ,  Kayoko Tanaka ,  Kohta Fujimori

IPC分类号： G03G15/02

CPC分类号： G03G15/0266 ,  G03G15/5058

摘要： An image forming apparatus includes a latent image bearer to rotate, an image forming unit including a charger and a developing device, a charge power supply to output a charging bias applied to a charger, a transfer device, a toner adhesion amount, and a controller. The controller causes the image forming unit to form a background fog pattern in a background area of the latent image bearer while changing a background potential, acquires toner adhesion amount values detected at different positions of the background fog pattern, having different potentials, sorts the toner adhesion amount values in an order of the background potential, determines a relation between the background potential and background fog amount based the toner adhesion amount values except any toner adhesion amount value out of monotonicity, and adjust the charging bias to an optimum value computed based on the determined relation.

公开(公告)号：US08945865B2

公开(公告)日：2015-02-03

申请号：US14003541

申请日：2012-03-06

申请人： Saki Izumi ,  Takafumi Komori ,  Yoshitane Nozaki

发明人： Saki Izumi ,  Takafumi Komori ,  Yoshitane Nozaki

IPC分类号： C12Q1/68 ,  G01N21/64 ,  G01N33/50 ,  G01N33/58 ,  G01N33/68

CPC分类号： G01N21/6486 ,  C09B11/24 ,  G01N33/5008 ,  G01N33/5067 ,  G01N33/582 ,  G01N33/6872 ,  G01N2500/00

摘要： The present invention provides a method of screening for a compound that enhances or inhibits the transport activity of OATP1B1, using dichlorofluorescein. The present invention also provides a use of dichlorofluorescein in measurement of the expression level of OATP1B1. The present invention further provides a method for measuring the expression level of OATP1B1 in test cells, using dichlorofluorescein. The present invention further provides a use of a kit including dichlorofluorescein and positive cells expressing OATP1B1 in measurement of the expression level of OATP1B1 in test cells.

摘要翻译： 本发明提供了使用二氯荧光素筛选增强或抑制OATP1B1的转运活性的化合物的方法。 本发明还提供了二氯荧光素在测量OATP1B1表达水平方面的用途。 本发明还提供了使用二氯荧光素测量测试细胞中OATP1B1的表达水平的方法。 本发明还提供了包含二氯荧光素和表达OATP1B1的阳性细胞的试剂盒在测试细胞中测量OATP1B1表达水平的用途。

公开(公告)号：US08895317B2

公开(公告)日：2014-11-25

申请号：US13131173

申请日：2009-12-04

申请人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

发明人： Tetsuo Nagano ,  Yasuteru Urano ,  Saki Izumi

IPC分类号： G01N33/48 ,  C07D311/82 ,  G01N31/22

CPC分类号： G01N31/22 ,  Y10T436/170769 ,  Y10T436/173076 ,  Y10T436/177692 ,  Y10T436/178459 ,  Y10T436/179228

摘要： A compound represented by the general formula (I) [R1 and R2 are amino groups that substitute at adjacent positions on the benzene ring; R3 and R4 are halogen atoms; R5 and R6 represent hydrogen atom, an acyl group or an acyloxy(C1-6 alkyl) group; R7 and R8 represent —(CH2)p—N(R9)(R10) (p is 1 to 4, and R9 and R10 represent —(CH2)n—COOH (n is 1 to 4))], which is useful for measuring a reactive nitrogen species existing in cells such as nitrogen monoxide or peroxynitrite at high sensitivity over a long period of time.

摘要翻译： 由通式（I）表示的化合物[R1和R2是在苯环上的相邻位置取代的氨基; R3和R4是卤素原子; R5和R6代表氢原子，酰基或酰氧基（C1-6烷基）基团; R7和R8表示 - （CH2）p-N（R9）（R10）（p为1至4，R9和R10表示 - （CH2）n-COOH（n为1至4））] 在长时间内以高灵敏度测量存在于细胞中的活性氮物质，例如一氧化氮或过氧亚硝酸盐。