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    METHOD FOR PRODUCING TARGET PROTEINS USING AMINO ACIDS AND PYRUVIC ACIDS IN CULTURE OF PLANT CELLS
    4.
    发明申请
    METHOD FOR PRODUCING TARGET PROTEINS USING AMINO ACIDS AND PYRUVIC ACIDS IN CULTURE OF PLANT CELLS 失效
    在植物细胞培养中使用氨基酸和吡啶酸生产目标蛋白的方法

    公开(公告)号：US20090181428A1

    公开(公告)日：2009-07-16

    申请号：US12160049

    申请日：2007-09-19

    申请人： Sang-Lin Kim ,  Hyun-Kwang Tan ,  Sang-Min Lim ,  Wuk-Sang Ryu ,  Hahn-Sun Jung ,  Song-Jae Lee ,  Cheon-Ik Park ,  Seung-Hoon Kang ,  Dong-Il Kim

    发明人： Sang-Lin Kim ,  Hyun-Kwang Tan ,  Sang-Min Lim ,  Wuk-Sang Ryu ,  Hahn-Sun Jung ,  Song-Jae Lee ,  Cheon-Ik Park ,  Seung-Hoon Kang ,  Dong-Il Kim

    IPC分类号： C12P21/00

    CPC分类号： C12P21/00 ,  A01H4/00 ,  C12N15/8237

    摘要： Provided is a method for producing a target protein via cultivation of transgenic plant cells comprising a promoter capable of expressing the protein under sugar-free conditions or in response to the depletion of sugar and a gene encoding the target protein, without exchange of a cell growth medium with a sugar-depleted medium. The method comprises 1) culturing transgenic plant cells in a sugar-rich medium to grow plant cells; and 2) culturing the transgenic plant cells with addition of an amino acid mixture to the culture of Step 1 without exchange of a cell growth medium with a sugar-depleted medium, thereby expressing a target protein. The method of the present invention enables commercial-scale production of recombinant proteins via establishment of optimized culture conditions by addition of an amino acid mixture to induce protein expression without exchange of a cell growth medium with a sugar-free medium, and addition of pyruvic acid during the induction period of protein expression to thereby enhance the production yield of target proteins.

    摘要翻译： 提供了通过培养转基因植物细胞来生产靶蛋白的方法，所述转基因植物细胞包含能够在无糖条件下表达该蛋白质的启动子，或者响应于糖的消耗和编码靶蛋白质的基因而不交换细胞生长 培养基含糖耗竭的培养基。 该方法包括：1）在富含糖的培养基中培养转基因植物细胞以生长植物细胞; 和2）向步骤1的培养物中加入氨基酸混合物的转基因植物细胞培养，而不用无糖培养基交换细胞生长培养基，从而表达靶蛋白。 本发明的方法可以通过添加氨基酸混合物建立优化的培养条件来产生重组蛋白质，从而诱导蛋白质表达而不用无糖培养基交换细胞生长培养基，并加入丙酮酸 在蛋白质表达的诱导期，从而提高靶蛋白的产量。

    Antifungal remedy formulation for external application
    5.
    发明授权
    Antifungal remedy formulation for external application 有权
    外用抗真菌药物配方

    公开(公告)号：US07485656B2

    公开(公告)日：2009-02-03

    申请号：US10467244

    申请日：2002-02-07

    申请人： Sang-Lin Kim ,  Hyun-Kwang Tan ,  Jun-Sik Kim ,  Ho-Beom Lee ,  Geun-Hong Min ,  Hae-Won Ryou

    发明人： Sang-Lin Kim ,  Hyun-Kwang Tan ,  Jun-Sik Kim ,  Ho-Beom Lee ,  Geun-Hong Min ,  Hae-Won Ryou

    IPC分类号： A61K31/4196 ,  A61K31/41 ,  A61K9/06

    CPC分类号： A61K9/0014 ,  A61K47/06 ,  A61K47/10 ,  A61K47/14 ,  A61K47/26

    摘要： An antibacterial composition for external application, particularly, a remedial formulation for external use containing fluconazole is disclosed. The formulation comprises a base material, 0.1-5.0 percent by weight of fluconazole and 1.0-40.0 percent by weight of any one compound selected from a group consisting of fatty acid, fatty alcohol, higher fatty acid ester and lower alcohol based on weight of the base material. The composition according to the present invention has various advantages of a favorable absorption into a system through the skin of a human body and delivery of active material into horny layer of the skin, a reduced skin stimulation and an excellent stability. Therefore, the composition is efficiently useable in a medical treatment application for dermatomycosis-related infectious diseases including tinea pedis, tinea corporis, tinea cruris, tinea versicolor and/or candidiasis and the like.

    摘要翻译： 公开了一种用于外用的抗菌组合物，特别是含有氟康唑的外用补救制剂。 该制剂包含基础材料，0.1-5.0重量％的氟康唑和1.0-40.0重量％的任何一种选自脂肪酸，脂肪醇，高级脂肪酸酯和低级醇的化合物，基于 基材。 根据本发明的组合物具有通过人体皮肤对体系的有利吸收以及将活性物质递送到皮肤角质层，减少的皮肤刺激和优异的稳定性的各种优点。 因此，该组合物可有效地用于涉及皮肤真菌病相关感染性疾病的医疗用途，包括足癣，癣，癣，癣斑和/或念珠菌病等。

    Plant Recombinant Human CTLA4IG and a Method for Producing the Same
    6.
    发明申请
    Plant Recombinant Human CTLA4IG and a Method for Producing the Same 审中-公开
    植物重组人CTLA4IG及其生产方法

    公开(公告)号：US20100189717A1

    公开(公告)日：2010-07-29

    申请号：US12668766

    申请日：2008-07-09

    申请人： Sang-Lin Kim ,  Hyun-Kwang Tan ,  Sang-Min Lim ,  Wuk-Sang Ryu ,  Hahn-Sun Jung ,  Song-Jae Lee ,  Cheon-Ik Park ,  Seung-Hoon Kang ,  Dong Il Kim

    发明人： Sang-Lin Kim ,  Hyun-Kwang Tan ,  Sang-Min Lim ,  Wuk-Sang Ryu ,  Hahn-Sun Jung ,  Song-Jae Lee ,  Cheon-Ik Park ,  Seung-Hoon Kang ,  Dong Il Kim

    IPC分类号： A61K39/395 ,  C12N15/74 ,  C12N5/10 ,  C12P21/00 ,  C07K16/18 ,  A61P37/04

    CPC分类号： C07K14/70521 ,  C07K2319/30 ,  C12N9/1051 ,  C12N15/8257 ,  C12P21/005

    摘要： The present invention provides a recombinant vector pBI-3D-hGalT or pBI-35S-hGalT containing a human β1,4-galactosyltransferase gene; a cell line transformed with a recombinant vector pMYN414 containing a cytotoxic T-lymphocyte anti-gen A-immunoglobulin (CTLA4Ig) fusion protein gene and the recombinant vector pBI-3D-hGalT or pBI-355-hGalT; and a method for producing a plant-derived recombinant human CTLA4Ig (CTLA4Igp) fusion protein with a human glycan structure using the same. The plant cell-derived recombinant human CTLA4Ig fusion protein (CTLA4Igp), which has a human glycan structure and is produced according to the present invention, exhibits an improved in vivo half life as compared to conventional plant-derived proteins, due to the presence of a human-like glycan structure. Consequently, the present invention using the plant expression system enables low-cost mass production of a CTLA4Igp fusion protein having an immunosuppressive activity comparable to that of the CTLA4IgM fusion protein expressed in conventional animal cell expression systems.

    摘要翻译： 本发明提供了含有人和重组1,4-半乳糖基转移酶基因的重组载体pBI-3D-hGalT或pBI-35S-hGalT; 用含有细胞毒性T淋巴细胞抗A型免疫球蛋白（CTLA4Ig）融合蛋白基因和重组载体pBI-3D-hGalT或pBI-355-hGalT的重组载体pMYN414转化的细胞系; 以及使用其使用具有人聚糖结构的植物来源的重组人CTLA4Ig（CTLA4Igp）融合蛋白的方法。 具有人聚糖结构并根据本发明生产的植物细胞衍生的重组人CTLA4Ig融合蛋白（CTLA4Igp）与常规植物衍生的蛋白质相比显示出与常规植物衍生的蛋白质相比改善的体内半衰期， 人类聚糖结构。 因此，使用植物表达系统的本发明能够低成本大规模生产具有与常规动物细胞表达系统中表达的CTLA4IgM融合蛋白相当的免疫抑制活性的CTLA4Igp融合蛋白。