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1.发明授权公开(公告)号：US07682788B2
公开(公告)日：2010-03-23
申请号：US10884536
申请日：2004-07-02
申请人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
发明人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
IPC分类号： C12Q1/68
CPC分类号： G01N33/54346 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , G01N33/532 , G01N33/533 , G01N33/54306 , G01N33/54366 , G01N33/5438 , G01N33/552 , G01N33/553 , G01N33/582 , G01N33/587 , G01N2458/30 , C12Q2563/149 , C12Q2563/113 , C12Q2537/125
摘要： A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
摘要翻译： 提出了一种用于进行电化学发光测定的方法和装置。 在本发明的示例性实施方案中，一种装置包括其上固定有第一测定配体的ECL非活性电极表面和其上固定有第二测定配体的ECL活性微粒。 在本发明的示例性实施方案中，方法包括（a）形成包含电化学发光标记和其上固定有第一测定配体的微粒的混合物，（b）在所述混合物存在下将电化学能施加到电极表面，以及 （c）测量发射的电化学发光，其中微粒是ECL-活性的，并且当在所述混合物的存在下施加电化学能时，电极表面是ECL-无活性的。
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2.发明授权Multi-label complex for enhanced sensitivity in electrochemiluminescence assay 失效用于增强电化学发光测定灵敏度的多标签复合物
公开(公告)号：US6096500A
公开(公告)日：2000-08-01
申请号：US954355
申请日：1997-10-20
IPC分类号： C12Q1/68 , G01N33/533 , G01N33/543 , G01N33/58
CPC分类号： G01N33/585 , C12Q1/6816 , G01N33/533 , G01N33/54306 , G01N33/582 , G01N2458/30
摘要： A nucleotide probe complex which enhances the ability to discriminate low level samples in electrochemiluminescent assays. The complex is composed of a platform molecule to which multiple copies of an organometallic electrochemiluminescent label and an oligonucleotide probe are separately attached. Preferably the complex is capped with streptavidin. Use of the complex permits detection of 1000 copies of analyte per sample in less than one hour.
摘要翻译： 核苷酸探针复合物，其增强在电化学发光测定中鉴别低水平样品的能力。 复合物由平台分子组成，分子连接有多个副产物的有机金属电化学发光标记和寡核苷酸探针。 优选地，复合物用链霉亲和素封端。 复合物的使用允许在不到一小时内检测每个样品1000份分析物。
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3.发明授权公开(公告)号：US09448235B2
公开(公告)日：2016-09-20
申请号：US12591528
申请日：2009-11-23
申请人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
发明人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
IPC分类号： G01N27/26 , G01N33/543 , B82Y30/00 , C07H21/00 , C12Q1/68 , G01N33/532 , G01N33/533 , G01N33/58
CPC分类号： G01N33/54346 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , G01N33/532 , G01N33/533 , G01N33/54306 , G01N33/54366 , G01N33/5438 , G01N33/552 , G01N33/553 , G01N33/582 , G01N33/587 , G01N2458/30 , C12Q2563/149 , C12Q2563/113 , C12Q2537/125
摘要： A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
摘要翻译： 提出了一种用于进行电化学发光测定的方法和装置。 在本发明的示例性实施方案中，一种装置包括其上固定有第一测定配体的ECL非活性电极表面和其上固定有第二测定配体的ECL活性微粒。 在本发明的示例性实施方案中，方法包括（a）形成包含电化学发光标记和其上固定有第一测定配体的微粒的混合物，（b）在所述混合物存在下将电化学能施加到电极表面，以及 （c）测量发射的电化学发光，其中微粒是ECL-活性的，并且当在所述混合物的存在下施加电化学能时，电极表面是ECL-无活性的。
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4.发明授权公开(公告)号：US06846629B2
公开(公告)日：2005-01-25
申请号：US09896962
申请日：2001-06-29
申请人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
发明人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
IPC分类号： C07H21/00 , C12Q1/68 , G01N33/533 , G01N33/543 , G01N33/58 , C07H19/00 , C07H21/04 , C12P19/34 , C07H21/07
CPC分类号： G01N33/54346 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , G01N33/532 , G01N33/533 , G01N33/54306 , G01N33/54366 , G01N33/5438 , G01N33/552 , G01N33/553 , G01N33/582 , G01N33/587 , G01N2458/30 , C12Q2563/149 , C12Q2563/113 , C12Q2537/125
摘要： Microparticles comprising a core and electrochemiluminescent moieties within the core and methods of using the same in assays.
摘要翻译： 核心内的核心和核心内的电化学发光部分以及在分析中使用该微粒子的方法。
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5.发明申请公开(公告)号：US20100140086A1
公开(公告)日：2010-06-10
申请号：US12591528
申请日：2009-11-23
申请人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
发明人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
CPC分类号： G01N33/54346 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , G01N33/532 , G01N33/533 , G01N33/54306 , G01N33/54366 , G01N33/5438 , G01N33/552 , G01N33/553 , G01N33/582 , G01N33/587 , G01N2458/30 , C12Q2563/149 , C12Q2563/113 , C12Q2537/125
摘要： A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
摘要翻译： 提出了一种用于进行电化学发光测定的方法和装置。 在本发明的示例性实施方案中，一种装置包括其上固定有第一测定配体的ECL非活性电极表面和其上固定有第二测定配体的ECL活性微粒。 在本发明的示例性实施方案中，方法包括（a）形成包含电化学发光标记和其上固定有第一测定配体的微粒的混合物，（b）在所述混合物存在下将电化学能施加到电极表面，以及 （c）测量发射的电化学发光，其中微粒是ECL-活性的，并且当在所述混合物的存在下施加电化学能时，电极表面是ECL-无活性的。
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6.发明授权公开(公告)号：US06319670B1
公开(公告)日：2001-11-20
申请号：US08998137
申请日：1997-12-23
申请人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
发明人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
IPC分类号： C12Q168
CPC分类号： G01N33/54346 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , G01N33/532 , G01N33/533 , G01N33/54306 , G01N33/54366 , G01N33/5438 , G01N33/552 , G01N33/553 , G01N33/582 , G01N33/587 , G01N2458/30 , C12Q2563/149 , C12Q2563/113 , C12Q2537/125
摘要： The present invention relates to methods, reagents and compositions, for conducting electrochemiluminescence binding assays which improve one or more characteristics of the assay or the instruments used to conduct the assay. The method is achieved using microparticles that include electrically conductive material. The electrically conductive material has one or more copies of an assay ligand immobilized an its outer surface and a plurality of electrochemiluminescent moieties immobilized an its outer surface. The assay ligand may be linked to the electrochemiluminescent moiety.
摘要翻译： 本发明涉及用于进行电化学发光结合测定法的方法，试剂和组合物，其改进测定的一个或多个特征或用于进行测定的仪器。 该方法使用包括导电材料的微粒来实现。 导电材料具有固定其外表面的测定配体的一个或多个拷贝和固定其外表面的多个电化学发光部分。 测定配体可以连接到电化学发光部分。
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