公开(公告)号：US08394417B2

公开(公告)日：2013-03-12

申请号：US12903615

申请日：2010-10-13

申请人： Yoichi Ishii ,  Kazuyuki Ishii ,  Hiroyuki Sumi ,  Etsuo Yoshida ,  Sayaka Ishii

发明人： Yoichi Ishii ,  Kazuyuki Ishii ,  Hiroyuki Sumi ,  Etsuo Yoshida ,  Sayaka Ishii

IPC分类号： A61K35/56

CPC分类号： A61K35/62

摘要： Provided is a simple and easy method of producing an innoxious dry earthworm powder, while suppressing inactivation of the enzymes contained in earthworms. In the method, a homogenate obtained by grinding living earthworms is freeze-dried and the dried product is heat-treated at a temperature of 110° C. or higher but lower than 130° C. Preferably, the method according to the present invention comprises further a process for preparation of the living earthworms, which precedes the grinding step, comprising the steps of: standing the living earthworms under light for 10 to 50 hours; removing the dirt formed on the skin thereof, bringing the earthworms into contact with an organic acid for 30 seconds or less; diluting the acid with addition of water to adjust the pH of the aqueous acidic solution to a pH in the range of 2 to 5; standing the earthworm mixture under the pH condition for 3 to 180 minutes; and then washing the earthworms with water.

摘要翻译： 提供了一种简单易行的生产无嗅干蚯蚓粉的方法，同时抑制蚯蚓中含有的酶的失活。 在该方法中，通过研磨活蚯蚓获得的匀浆物被冷冻干燥，干燥产物在110℃以上且低于130℃的温度下进行热处理。优选地，根据本发明的方法包括 进一步的在研磨步骤之前制备活蚯蚓的方法，包括以下步骤：将活蚯蚓放在灯下10-50小时; 除去形成在其皮肤上的污垢，使蚯蚓与有机酸接触30秒以下; 通过添加水稀释酸，以将酸性水溶液的pH调节至2至5范围内的pH; 在pH条件下静置蚯蚓混合物3〜180分钟; 然后用水洗蚯蚓。

公开(公告)号：US20100276359A1

公开(公告)日：2010-11-04

申请号：US12677955

申请日：2008-09-08

申请人： Masamichi Ippommatsu ,  Chie Kurusu ,  Riichi Miyamoto ,  Sayaka Ishii ,  Kazuki Nakanishi ,  Hiroyoshi Minakuchi ,  Keiko Yano

发明人： Masamichi Ippommatsu ,  Chie Kurusu ,  Riichi Miyamoto ,  Sayaka Ishii ,  Kazuki Nakanishi ,  Hiroyoshi Minakuchi ,  Keiko Yano

IPC分类号： B01D15/22

CPC分类号： A61M1/3679 ,  B01J20/103 ,  B01J20/2808 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/28095 ,  B01J20/3204 ,  B01J20/327 ,  B01J20/3274 ,  B01J20/328 ,  B01J2220/58

摘要： Provided is an absorption column for body fluid purification by which the saturated adsorption amount of a substance to be adsorbed can be increased or the time required for reaching the saturated adsorption can be shortened, and therefore, the column capacity can be considerably reduced and the treatment time can be considerably shortened. The adsorption column having functional groups 6 specifically binding to a substance to be adsorbed including at least low density lipoprotein, immobilized on the surface of a porous support 5. The porous support 5 has double-pore structure comprising: a skeleton 7 made of a silica gel or silica glass having a three-dimensional network structure; through-pores 8 of an average diameter measured by the mercury intrusion method of 1 μm or more but less than 4 μm and having a three-dimensional network structure, which are formed in the voids of the skeleton; and micropores 9 having an average diameter measured by the mercury intrusion method larger than the grain diameter of the substance to be adsorbed, which are dispersedly formed on the surface of the skeleton.

摘要翻译： 本发明提供一种用于体液净化的吸收塔，其中吸附物质的饱和吸附量可以增加或达到饱和吸附所需的时间可以缩短，因此可以显着降低色谱柱容量和处理 时间可以大大缩短。 该吸附塔具有特定结合待吸附物质的官能团6，该物质固定在多孔载体5的表面上，至少含有低密度脂蛋白。多孔载体5具有双孔结构，包括：由二氧化硅 具有三维网络结构的凝胶或二氧化硅玻璃; 通过水银侵入法测量的平均直径为1μm以上且小于4μm的具有三维网状结构的通孔8，其形成在骨架的空隙中; 并且具有通过水银侵入法测量的平均直径的微孔9大于分散形成在骨架表面上的待吸附物质的粒径。

公开(公告)号：US20110086106A1

公开(公告)日：2011-04-14

申请号：US12903615

申请日：2010-10-13

申请人： Yoichi ISHII ,  Kazuyuki Ishii ,  Hiroyuki Sumi ,  Etsuo Yoshida ,  Sayaka Ishii

发明人： Yoichi ISHII ,  Kazuyuki Ishii ,  Hiroyuki Sumi ,  Etsuo Yoshida ,  Sayaka Ishii

IPC分类号： A61K35/56

CPC分类号： A61K35/62

摘要： Provided is a simple and easy method of producing an innoxious dry earthworm powder, while suppressing inactivation of the enzymes contained in earthworms. In the method, a homogenate obtained by grinding living earthworms is freeze-dried and the dried product is heat-treated at a temperature of 110° C. or higher but lower than 130° C. Preferably, the method according to the present invention comprises further a process for preparation of the living earthworms, which precedes the grinding step, comprising the steps of: standing the living earthworms under light for 10 to 50 hours; removing the dirt formed on the skin thereof, bringing the earthworms into contact with an organic acid for 30 seconds or less; diluting the acid with addition of water to adjust the pH of the aqueous acidic solution to a pH in the range of 2 to 5; standing the earthworm mixture under the pH condition for 3 to 180 minutes; and then washing the earthworms with water.

摘要翻译： 提供了一种简单易行的生产无嗅干蚯蚓粉的方法，同时抑制蚯蚓中含有的酶的失活。 在该方法中，通过研磨活蚯蚓获得的匀浆物被冷冻干燥，干燥产物在110℃以上且低于130℃的温度下进行热处理。优选地，根据本发明的方法包括 进一步的在研磨步骤之前制备活蚯蚓的方法，包括以下步骤：将活蚯蚓放在灯下10-50小时; 除去形成在其皮肤上的污垢，使蚯蚓与有机酸接触30秒以下; 通过添加水稀释酸，以将酸性水溶液的pH调节至2至5范围内的pH; 在pH条件下静置蚯蚓混合物3〜180分钟; 然后用水洗蚯蚓。