利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

2 条记录 (耗时 0.013 秒)

    Method of Manufacturing Induced Pluripotent Stem Cell Originated from Somatic Cell
    1.
    发明申请
    Method of Manufacturing Induced Pluripotent Stem Cell Originated from Somatic Cell 审中-公开
    诱导多能干细胞来源于体细胞的方法

    公开(公告)号：US20110129928A1

    公开(公告)日：2011-06-02

    申请号：US12865690

    申请日：2008-01-31

    申请人： Se Pill Park ,  Eun Young Kim ,  Kilsoo Jeon ,  Ssang-Goo Cho

    发明人： Se Pill Park ,  Eun Young Kim ,  Kilsoo Jeon ,  Ssang-Goo Cho

    IPC分类号： C12N15/86

    CPC分类号： C12N5/0696 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2510/00

    摘要： Disclosed is a method for manufacturing stem cells including preparing Oct-4 gene, Sox2 gene, C-myc gene, and Klf-4 gene from mouse embryonic stem cells, and allowing each of the genes to be infected in host cells using a lentiviral vector system to generate viruses in which each of the genes are induced; concentrating or mixing each of the viruses to prepare a virus concentrated mixture, and mixing the virus concentrated mixture and a first culture solution to prepare a virus solution; floating mouse somatic cells having been cultivated in advance in a first culture dish, and mixing and reacting the floated somatic cells and the virus solution to prepare a somatic cell-virus mixture; adding and retaining the somatic cell-virus mixture as is in a second culture dish including a second culture solution to induce the genes in the somatic cells; and cultivating the somatic cells.

    摘要翻译： 公开了一种制造干细胞的方法，包括从小鼠胚胎干细胞制备Oct-4基因，Sox2基因，C-myc基因和Klf-4基因，并且使用慢病毒载体使宿主细胞中的每一种都被感染 系统产生其中每个基因被诱导的病毒; 浓缩或混合每种病毒以制备病毒浓缩混合物，并混合病毒浓缩混合物和第一培养溶液以制备病毒溶液; 在第一培养皿中预先培养的浮游小鼠体细胞，并使浮选的体细胞和病毒溶液混合和反应以制备体细胞 - 病毒混合物; 在包含第二培养液的第二培养皿中加入和保留体细胞 - 病毒混合物以诱导体细胞中的基因; 并培养体细胞。