公开(公告)号：US07745203B2

公开(公告)日：2010-06-29

申请号：US11045646

申请日：2005-01-31

申请人： Sadato Hongo ,  Minoru Ishikawa ,  Takiji Ishimura ,  Shigeru Wakayama ,  Shinji Yanaga ,  Kenji Oki ,  Jun Okada

发明人： Sadato Hongo ,  Minoru Ishikawa ,  Takiji Ishimura ,  Shigeru Wakayama ,  Shinji Yanaga ,  Kenji Oki ,  Jun Okada

IPC分类号： C12M1/00 ,  C12M1/34 ,  C12M3/00

CPC分类号： G01N33/5438

摘要： A base sequence detection apparatus is provided with a channel formed on a base sequence detection chip. Working electrodes are formed along the channel and include a probe immobilized thereon, counter electrodes are formed on the inner surface of the channel and, reference electrodes are formed on the inner surface of the channel. An introduction port introduces solution or air from the upstream side of the channel, a delivery port delivers the solution or air in the channel, and the sample is injected into the channel through a sample injection port.

摘要翻译： 碱基序列检测装置具有形成在基序列检测芯片上的信道。 沿通道形成工作电极，并且包括固定在其上的探针，在通道的内表面上形成相对电极，并且在通道的内表面上形成参考电极。 引入端口从通道的上游侧引入溶液或空气，输送口将溶液或空气输送到通道中，并且样品通过样品注入口注入通道。

公开(公告)号：US08150635B2

公开(公告)日：2012-04-03

申请号：US12949411

申请日：2010-11-18

申请人： Sadato Hongo ,  Shinji Yanaga

发明人： Sadato Hongo ,  Shinji Yanaga

IPC分类号： G06F19/00 ,  G01N33/48 ,  G01N31/00 ,  C12Q1/68

CPC分类号： G01N27/3275

摘要： A method for determining nucleotide sequence encompasses: injecting a solution containing a sample DNA into a chip cartridge provided with a detecting electrode, to which a probe DNA is immobilized; introducing an intercalator solution in the chip cartridge; obtaining a current-voltage characteristic curve by measuring a current in the solution due to an electrochemical reaction of the intercalator through the detecting electrode; obtaining a baseline by linearly approximating the current-voltage characteristic curve; obtaining a net current value by subtracting, from a peak current value of the current-voltage characteristic curve, a baseline current value obtained from the baseline at a peak voltage value defining the peak current value; and identifying a nucleotide sequence in the sample DNA, using the net current value.

摘要翻译： 确定核苷酸序列的方法包括：将含有样本DNA的溶液注入设置有检测电极的芯片盒中，固定有探针DNA; 在芯片盒中引入嵌入剂溶液; 通过测量由于嵌入剂通过检测电极的电化学反应而导致的溶液中的电流来获得电流 - 电压特性曲线; 通过线性近似电流 - 电压特性曲线获得基线; 通过从当前电压特性曲线的峰值电流值中减去在定义峰值电流值的峰值电压值处从基线获得的基线电流值来获得净电流值; 并使用净电流值鉴定样品DNA中的核苷酸序列。

公开(公告)号：US20060252067A1

公开(公告)日：2006-11-09

申请号：US11377265

申请日：2006-03-17

申请人： Sadato Hongo ,  Shinji Yanaga

发明人： Sadato Hongo ,  Shinji Yanaga

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： G01N27/3275

摘要： A method for determining nucleotide sequence encompasses: injecting a solution containing a sample DNA into a chip cartridge provided with a detecting electrode, to which a probe DNA is immobilized; introducing an intercalator solution in the chip cartridge; obtaining a current-voltage characteristic curve by measuring a current in the solution due to an electrochemical reaction of the intercalator through the detecting electrode; obtaining a baseline by linearly approximating the current-voltage characteristic curve; obtaining a net current value by subtracting, from a peak current value of the current-voltage characteristic curve, a baseline current value obtained from the baseline at a peak voltage value defining the peak current value; and identifying a nucleotide sequence in the sample DNA, using the net current value.

摘要翻译： 确定核苷酸序列的方法包括：将含有样本DNA的溶液注入设置有检测电极的芯片盒中，固定有探针DNA; 在芯片盒中引入嵌入剂溶液; 通过测量由于嵌入剂通过检测电极的电化学反应而导致的溶液中的电流来获得电流 - 电压特性曲线; 通过线性近似电流 - 电压特性曲线获得基线; 通过从当前电压特性曲线的峰值电流值中减去在定义峰值电流值的峰值电压值处从基线获得的基线电流值来获得净电流值; 并使用净电流值鉴定样品DNA中的核苷酸序列。

公开(公告)号：US20110127171A1

公开(公告)日：2011-06-02

申请号：US12949411

申请日：2010-11-18

申请人： Sadato HONGO ,  Shinji YANAGA

发明人： Sadato HONGO ,  Shinji YANAGA

IPC分类号： G01N27/416

CPC分类号： G01N27/3275

摘要： A method for determining nucleotide sequence encompasses: injecting a solution containing a sample DNA into a chip cartridge provided with a detecting electrode, to which a probe DNA is immobilized; introducing an intercalator solution in the chip cartridge; obtaining a current-voltage characteristic curve by measuring a current in the solution due to an electrochemical reaction of the intercalator through the detecting electrode; obtaining a baseline by linearly approximating the current-voltage characteristic curve; obtaining a net current value by subtracting, from a peak current value of the current-voltage characteristic curve, a baseline current value obtained from the baseline at a peak voltage value defining the peak current value; and identifying a nucleotide sequence in the sample DNA, using the net current value.

摘要翻译： 确定核苷酸序列的方法包括：将含有样本DNA的溶液注入设置有检测电极的芯片盒中，固定有探针DNA; 在芯片盒中引入嵌入剂溶液; 通过测量由于嵌入剂通过检测电极的电化学反应而导致的溶液中的电流来获得电流 - 电压特性曲线; 通过线性近似电流 - 电压特性曲线获得基线; 通过从当前电压特性曲线的峰值电流值中减去在定义峰值电流值的峰值电压值处从基线获得的基线电流值来获得净电流值; 并使用净电流值鉴定样品DNA中的核苷酸序列。

公开(公告)号：US20080060940A1

公开(公告)日：2008-03-13

申请号：US11980409

申请日：2007-10-31

申请人： Sadato Hongo ,  Minoru Ishikawa ,  Takiji Ishimura ,  Shigeru Wakayama ,  Shinji Yanaga ,  Kenji Oki ,  Jun Okada

发明人： Sadato Hongo ,  Minoru Ishikawa ,  Takiji Ishimura ,  Shigeru Wakayama ,  Shinji Yanaga ,  Kenji Oki ,  Jun Okada

IPC分类号： G01N27/416

CPC分类号： G01N33/5438

摘要： A base sequence detection apparatus is provided with a channel formed on a base sequence detection chip. Working electrodes are formed along the channel and include a probe immobilized thereon, counter electrodes are formed on the inner surface of the channel and, reference electrodes are formed on the inner surface of the channel. An introduction port introduces solution or air from the upstream side of the channel, a delivery port delivers the solution or air in the channel, and the sample is injected into the channel through a sample injection port.

摘要翻译： 碱基序列检测装置具有形成在基序列检测芯片上的信道。 沿通道形成工作电极，并且包括固定在其上的探针，在通道的内表面上形成相对电极，并且在通道的内表面上形成参考电极。 引入端口从通道的上游侧引入溶液或空气，输送口将溶液或空气输送到通道中，并且样品通过样品注入口注入通道。

公开(公告)号：US20100121490A1

公开(公告)日：2010-05-13

申请号：US12689716

申请日：2010-01-19

申请人： Sadato HONGO ,  Minoru ISHIKAWA ,  Takiji ISHIMURA ,  Shigeru WAKAYAMA ,  Shinji YANAGA ,  Kenji OKI ,  Jun OKADA

发明人： Sadato HONGO ,  Minoru ISHIKAWA ,  Takiji ISHIMURA ,  Shigeru WAKAYAMA ,  Shinji YANAGA ,  Kenji OKI ,  Jun OKADA

IPC分类号： G05B21/00 ,  G01N27/26

CPC分类号： G01N33/5438

摘要： A base sequence detection apparatus is provided with a channel formed on a base sequence detection chip. Working electrodes are formed along the channel and include a probe immobilized thereon, counter electrodes are formed on the inner surface of the channel and, reference electrodes are formed on the inner surface of the channel. An introduction port introduces solution or air from the upstream side of the channel, a delivery port delivers the solution or air in the channel, and the sample is injected into the channel through a sample injection port.

摘要翻译： 碱基序列检测装置具有形成在基序列检测芯片上的信道。 沿通道形成工作电极，并且包括固定在其上的探针，在通道的内表面上形成相对电极，并且在通道的内表面上形成参考电极。 引入端口从通道的上游侧引入溶液或空气，输送口将溶液或空气输送到通道中，并且样品通过样品注入口注入通道。

公开(公告)号：US20050158787A1

公开(公告)日：2005-07-21

申请号：US11045646

申请日：2005-01-31

申请人： Sadato Hongo ,  Minoru Ishikawa ,  Takiji Ishimura ,  Shigeru Wakayama ,  Shinji Yanaga ,  Kenji Oki ,  Jun Okada

发明人： Sadato Hongo ,  Minoru Ishikawa ,  Takiji Ishimura ,  Shigeru Wakayama ,  Shinji Yanaga ,  Kenji Oki ,  Jun Okada

IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/543

CPC分类号： G01N33/5438

摘要： A base sequence detection apparatus is provided with a channel formed on a base sequence detection chip. Working electrodes are formed along the channel and include a probe immobilized thereon, counter electrodes are formed on the inner surface of the channel and, reference electrodes are formed on the inner surface of the channel. An introduction port introduces solution or air from the upstream side of the channel, a delivery port delivers the solution or air in the channel, and the sample is injected into the channel through a sample injection port.

摘要翻译： 碱基序列检测装置具有形成在基序列检测芯片上的信道。 沿通道形成工作电极，并且包括固定在其上的探针，在通道的内表面上形成相对电极，并且在通道的内表面上形成参考电极。 引入端口从通道的上游侧引入溶液或空气，输送口将溶液或空气输送到通道中，并且样品通过样品注入口注入通道。