公开(公告)号：US20130101635A1

公开(公告)日：2013-04-25

申请号：US13650670

申请日：2012-10-12

申请人： Kwideok PARK ,  Soon Eon BAE ,  Dong Keun HAN

发明人： Kwideok PARK ,  Soon Eon BAE ,  Dong Keun HAN

IPC分类号： C12N5/00 ,  A61K35/32 ,  A61K35/28

CPC分类号： C12N5/0062 ,  A61K35/28 ,  A61K35/32 ,  C12N2533/90

摘要： Methods of preparing a self-assembled matrix from cell-derived extracellular matrice, and of inducing cell proliferation and differentiation by using the self-assembled matrix, and applying the self-assembled matrix into cell therapy. In detail, unique extracellular matrices obtained from particular cells are collected and then subjected to decellularization in order to form a self-assembled matrix including nano fibers only formed of extracellular matrix. In addition, the matrix prepared as described above provides a platform that is effective for the induction of the mass proliferation or differentiation of cells, and thus, may be applied in manufacturing cell therapy products.

摘要翻译： 从细胞衍生的细胞外基质制备自组装基质的方法，以及通过使用自组装基质诱导细胞增殖和分化，以及将自组装基质应用于细胞治疗。 详细地，从特定细胞获得的独特的细胞外基质被收集，然后进行脱细胞化以形成仅由细胞外基质形成的纳米纤维的自组装基质。 此外，如上所述制备的基质提供了对诱导细胞的大量增殖或分化有效的平台，因此可以应用于制造细胞治疗产品。

公开(公告)号：US08956643B2

公开(公告)日：2015-02-17

申请号：US12473850

申请日：2009-05-28

申请人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim ,  Soon Eon Bae

发明人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim ,  Soon Eon Bae

IPC分类号： A61F2/00 ,  A61F13/00 ,  A61L27/50 ,  A61L27/18 ,  A61L27/54 ,  A61L27/56

CPC分类号： A61L27/50 ,  A61L27/18 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/25 ,  A61L2300/414 ,  A61L2300/428 ,  A61L2300/43 ,  A61L2300/45 ,  A61L2300/604 ,  A61L2400/18 ,  C08L67/04

摘要： The present invention relates to an intelligent porous biodegradable polymer scaffold for in situ tissue regeneration in which two kinds of physiologically active substances having high differentiation potential and biocompatibility are introduced into the polymer scaffold and conjugated to the surface thereof, respectively, and a method for the preparation thereof. Since the intelligent porous biodegradable polymer scaffold exhibits improved biocompatibility and differentiation potential due to the introduction of physiologically active substances capable of efficiently inducing cell proliferation and differentiation into both the surface and the inside thereof, it can directly induce in situ tissue regeneration of the musculoskeletal system from stem cells in a living tissue after transplanting the polymer scaffold and stem cells into a human body without additional in vitro cultivation. Therefore, the intelligent porous biodegradable polymer scaffold according to the present invention can be effectively used in the regeneration of various kinds of tissues and organs including the musculoskeletal system.

摘要翻译： 本发明涉及一种用于原位组织再生的智能多孔可生物降解聚合物支架，其中将具有高分化潜能和生物相容性的两种生理活性物质分别引入聚合物支架并与其表面共轭， 的制备。 由于智能多孔生物可降解聚合物支架由于引入能够有效诱导细胞增殖和分化至其表面和内部的生理活性物质而显示出改善的生物相容性和分化潜能，因此可直接诱导肌肉骨骼系统的原位组织再生 从移植聚合物支架和干细胞到人体中的生物体组织中的干细胞，而不需要额外的体外培养。 因此，根据本发明的智能多孔生物可降解聚合物支架可以有效地用于包括肌肉骨骼系统在内的各种组织和器官的再生。

公开(公告)号：US20090317478A1

公开(公告)日：2009-12-24

申请号：US12255163

申请日：2008-10-21

申请人： Dong Keun HAN ,  Kwideok Park ,  Jae-Jin Kim ,  Jun Sik Son ,  Soon Eon Bae

发明人： Dong Keun HAN ,  Kwideok Park ,  Jae-Jin Kim ,  Jun Sik Son ,  Soon Eon Bae

IPC分类号： A61K9/14 ,  A61K38/21

CPC分类号： A61K9/1647 ,  A61K9/1694 ,  A61K38/00

摘要： The present invention relates to covered porous biodegradable polymer microspheres for sustained-release drug delivery and tissue regeneration which has an interconnected inner open pore structure having a wide surface area and high porosity and an outer closed pore structure in which the surface of the microsphere is covered with a thin layer of a biodegradable polymer, and thereby the pores formed on the surface and exposed to the outside are closed; and methods for preparing the same. Due to such a characteristic pore structure, the covered porous biodegradable polymer microspheres according to the present invention can prevent the biologically active material from being excessively released in the early stage immediately after administration, and after that, can gradually release the biologically active material through the interconnected inner pore structure over a prolonged period as the biodegradable polymer thin layer is degraded. Therefore, the microsphere of the present invention can be effectively used for sustained-release drug delivery and tissue regeneration.

摘要翻译： 本发明涉及用于持续释放药物递送和组织再生的覆盖多孔可生物降解聚合物微球，其具有互连的具有宽表面积和高孔隙率的内开孔结构以及微球表面被覆盖的外封闭孔结构 具有可生物降解聚合物的薄层，从而形成在表面上并暴露于外部的孔闭合; 及其制备方法。 由于这样的特征性的孔结构，本发明的被覆多孔生物降解性聚合物微球体可以防止生物活性物质在给药后立即早期过度释放，之后可以通过使用生物活性物质逐渐释放生物活性物质 随着可生物降解的聚合物薄层的退化，延长的互连内孔结构。 因此，本发明的微球体可以有效地用于缓释药物递送和组织再生。

公开(公告)号：US08192655B2

公开(公告)日：2012-06-05

申请号：US12255163

申请日：2008-10-21

申请人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim ,  Jun Sik Son ,  Soon Eon Bae

发明人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim ,  Jun Sik Son ,  Soon Eon Bae

IPC分类号： B29B9/00

CPC分类号： A61K9/1647 ,  A61K9/1694 ,  A61K38/00

摘要： The present invention relates to covered porous biodegradable polymer microspheres for sustained-release drug delivery and tissue regeneration which has an interconnected inner open pore structure having a wide surface area and high porosity and an outer closed pore structure in which the surface of the microsphere is covered with a thin layer of a biodegradable polymer, and thereby the pores formed on the surface and exposed to the outside are closed; and methods for preparing the same. Due to such a characteristic pore structure, the covered porous biodegradable polymer microspheres according to the present invention can prevent the biologically active material from being excessively released in the early stage immediately after administration, and after that, can gradually release the biologically active material through the interconnected inner pore structure over a prolonged period as the biodegradable polymer thin layer is degraded. Therefore, the microsphere of the present invention can be effectively used for sustained-release drug delivery and tissue regeneration.

摘要翻译： 本发明涉及用于持续释放药物递送和组织再生的覆盖多孔可生物降解聚合物微球，其具有互连的具有宽表面积和高孔隙率的内开孔结构以及微球表面被覆盖的外封闭孔结构 具有可生物降解聚合物的薄层，从而形成在表面上并暴露于外部的孔闭合; 及其制备方法。 由于这样的特征性的孔结构，本发明的被覆多孔生物降解性聚合物微球体可以防止生物活性物质在给药后立即早期过度释放，之后可以通过使用生物活性物质逐渐释放生物活性物质 随着可生物降解的聚合物薄层的退化，延长的互连内孔结构。 因此，本发明的微球体可以有效地用于缓释药物递送和组织再生。

公开(公告)号：US20100129422A1

公开(公告)日：2010-05-27

申请号：US12473850

申请日：2009-05-28

申请人： Dong Keun HAN ,  Kwideok Park ,  Jae-Jin Kim ,  Soon Eon Bae

发明人： Dong Keun HAN ,  Kwideok Park ,  Jae-Jin Kim ,  Soon Eon Bae

IPC分类号： A61F2/00

CPC分类号： A61L27/50 ,  A61L27/18 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/25 ,  A61L2300/414 ,  A61L2300/428 ,  A61L2300/43 ,  A61L2300/45 ,  A61L2300/604 ,  A61L2400/18 ,  C08L67/04

摘要： The present invention relates to an intelligent porous biodegradable polymer scaffold for in situ tissue regeneration in which two kinds of physiologically active substances having high differentiation potential and biocompatibility are introduced into the polymer scaffold and conjugated to the surface thereof, respectively, and a method for the preparation thereof. Since the intelligent porous biodegradable polymer scaffold exhibits improved biocompatibility and differentiation potential due to the introduction of physiologically active substances capable of efficiently inducing cell proliferation and differentiation into both the surface and the inside thereof, it can directly induce in situ tissue regeneration of the musculoskeletal system from stem cells in a living tissue after transplanting the polymer scaffold and stem cells into a human body without additional in vitro cultivation. Therefore, the intelligent porous biodegradable polymer scaffold according to the present invention can be effectively used in the regeneration of various kinds of tissues and organs including the musculoskeletal system.

摘要翻译： 本发明涉及一种用于原位组织再生的智能多孔可生物降解聚合物支架，其中将具有高分化潜能和生物相容性的两种生理活性物质分别引入聚合物支架并与其表面共轭， 的制备。 由于智能多孔生物可降解聚合物支架由于引入能够有效诱导细胞增殖和分化至其表面和内部的生理活性物质而显示出改善的生物相容性和分化潜能，因此可直接诱导肌肉骨骼系统的原位组织再生 从移植聚合物支架和干细胞到人体中的生物体组织中的干细胞，而不需要额外的体外培养。 因此，根据本发明的智能多孔生物可降解聚合物支架可以有效地用于包括肌肉骨骼系统在内的各种组织和器官的再生。