公开(公告)号：US20100129422A1

公开(公告)日：2010-05-27

申请号：US12473850

申请日：2009-05-28

申请人： Dong Keun HAN ,  Kwideok Park ,  Jae-Jin Kim ,  Soon Eon Bae

发明人： Dong Keun HAN ,  Kwideok Park ,  Jae-Jin Kim ,  Soon Eon Bae

IPC分类号： A61F2/00

CPC分类号： A61L27/50 ,  A61L27/18 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/25 ,  A61L2300/414 ,  A61L2300/428 ,  A61L2300/43 ,  A61L2300/45 ,  A61L2300/604 ,  A61L2400/18 ,  C08L67/04

摘要： The present invention relates to an intelligent porous biodegradable polymer scaffold for in situ tissue regeneration in which two kinds of physiologically active substances having high differentiation potential and biocompatibility are introduced into the polymer scaffold and conjugated to the surface thereof, respectively, and a method for the preparation thereof. Since the intelligent porous biodegradable polymer scaffold exhibits improved biocompatibility and differentiation potential due to the introduction of physiologically active substances capable of efficiently inducing cell proliferation and differentiation into both the surface and the inside thereof, it can directly induce in situ tissue regeneration of the musculoskeletal system from stem cells in a living tissue after transplanting the polymer scaffold and stem cells into a human body without additional in vitro cultivation. Therefore, the intelligent porous biodegradable polymer scaffold according to the present invention can be effectively used in the regeneration of various kinds of tissues and organs including the musculoskeletal system.

摘要翻译： 本发明涉及一种用于原位组织再生的智能多孔可生物降解聚合物支架，其中将具有高分化潜能和生物相容性的两种生理活性物质分别引入聚合物支架并与其表面共轭， 的制备。 由于智能多孔生物可降解聚合物支架由于引入能够有效诱导细胞增殖和分化至其表面和内部的生理活性物质而显示出改善的生物相容性和分化潜能，因此可直接诱导肌肉骨骼系统的原位组织再生 从移植聚合物支架和干细胞到人体中的生物体组织中的干细胞，而不需要额外的体外培养。 因此，根据本发明的智能多孔生物可降解聚合物支架可以有效地用于包括肌肉骨骼系统在内的各种组织和器官的再生。

公开(公告)号：US20090317478A1

公开(公告)日：2009-12-24

申请号：US12255163

申请日：2008-10-21

申请人： Dong Keun HAN ,  Kwideok Park ,  Jae-Jin Kim ,  Jun Sik Son ,  Soon Eon Bae

发明人： Dong Keun HAN ,  Kwideok Park ,  Jae-Jin Kim ,  Jun Sik Son ,  Soon Eon Bae

IPC分类号： A61K9/14 ,  A61K38/21

CPC分类号： A61K9/1647 ,  A61K9/1694 ,  A61K38/00

摘要： The present invention relates to covered porous biodegradable polymer microspheres for sustained-release drug delivery and tissue regeneration which has an interconnected inner open pore structure having a wide surface area and high porosity and an outer closed pore structure in which the surface of the microsphere is covered with a thin layer of a biodegradable polymer, and thereby the pores formed on the surface and exposed to the outside are closed; and methods for preparing the same. Due to such a characteristic pore structure, the covered porous biodegradable polymer microspheres according to the present invention can prevent the biologically active material from being excessively released in the early stage immediately after administration, and after that, can gradually release the biologically active material through the interconnected inner pore structure over a prolonged period as the biodegradable polymer thin layer is degraded. Therefore, the microsphere of the present invention can be effectively used for sustained-release drug delivery and tissue regeneration.

摘要翻译： 本发明涉及用于持续释放药物递送和组织再生的覆盖多孔可生物降解聚合物微球，其具有互连的具有宽表面积和高孔隙率的内开孔结构以及微球表面被覆盖的外封闭孔结构 具有可生物降解聚合物的薄层，从而形成在表面上并暴露于外部的孔闭合; 及其制备方法。 由于这样的特征性的孔结构，本发明的被覆多孔生物降解性聚合物微球体可以防止生物活性物质在给药后立即早期过度释放，之后可以通过使用生物活性物质逐渐释放生物活性物质 随着可生物降解的聚合物薄层的退化，延长的互连内孔结构。 因此，本发明的微球体可以有效地用于缓释药物递送和组织再生。

公开(公告)号：US08192655B2

公开(公告)日：2012-06-05

申请号：US12255163

申请日：2008-10-21

申请人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim ,  Jun Sik Son ,  Soon Eon Bae

发明人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim ,  Jun Sik Son ,  Soon Eon Bae

IPC分类号： B29B9/00

CPC分类号： A61K9/1647 ,  A61K9/1694 ,  A61K38/00

摘要： The present invention relates to covered porous biodegradable polymer microspheres for sustained-release drug delivery and tissue regeneration which has an interconnected inner open pore structure having a wide surface area and high porosity and an outer closed pore structure in which the surface of the microsphere is covered with a thin layer of a biodegradable polymer, and thereby the pores formed on the surface and exposed to the outside are closed; and methods for preparing the same. Due to such a characteristic pore structure, the covered porous biodegradable polymer microspheres according to the present invention can prevent the biologically active material from being excessively released in the early stage immediately after administration, and after that, can gradually release the biologically active material through the interconnected inner pore structure over a prolonged period as the biodegradable polymer thin layer is degraded. Therefore, the microsphere of the present invention can be effectively used for sustained-release drug delivery and tissue regeneration.

摘要翻译： 本发明涉及用于持续释放药物递送和组织再生的覆盖多孔可生物降解聚合物微球，其具有互连的具有宽表面积和高孔隙率的内开孔结构以及微球表面被覆盖的外封闭孔结构 具有可生物降解聚合物的薄层，从而形成在表面上并暴露于外部的孔闭合; 及其制备方法。 由于这样的特征性的孔结构，本发明的被覆多孔生物降解性聚合物微球体可以防止生物活性物质在给药后立即早期过度释放，之后可以通过使用生物活性物质逐渐释放生物活性物质 随着可生物降解的聚合物薄层的退化，延长的互连内孔结构。 因此，本发明的微球体可以有效地用于缓释药物递送和组织再生。

公开(公告)号：US08956643B2

公开(公告)日：2015-02-17

申请号：US12473850

申请日：2009-05-28

申请人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim ,  Soon Eon Bae

发明人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim ,  Soon Eon Bae

IPC分类号： A61F2/00 ,  A61F13/00 ,  A61L27/50 ,  A61L27/18 ,  A61L27/54 ,  A61L27/56

CPC分类号： A61L27/50 ,  A61L27/18 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/25 ,  A61L2300/414 ,  A61L2300/428 ,  A61L2300/43 ,  A61L2300/45 ,  A61L2300/604 ,  A61L2400/18 ,  C08L67/04

摘要： The present invention relates to an intelligent porous biodegradable polymer scaffold for in situ tissue regeneration in which two kinds of physiologically active substances having high differentiation potential and biocompatibility are introduced into the polymer scaffold and conjugated to the surface thereof, respectively, and a method for the preparation thereof. Since the intelligent porous biodegradable polymer scaffold exhibits improved biocompatibility and differentiation potential due to the introduction of physiologically active substances capable of efficiently inducing cell proliferation and differentiation into both the surface and the inside thereof, it can directly induce in situ tissue regeneration of the musculoskeletal system from stem cells in a living tissue after transplanting the polymer scaffold and stem cells into a human body without additional in vitro cultivation. Therefore, the intelligent porous biodegradable polymer scaffold according to the present invention can be effectively used in the regeneration of various kinds of tissues and organs including the musculoskeletal system.

摘要翻译： 本发明涉及一种用于原位组织再生的智能多孔可生物降解聚合物支架，其中将具有高分化潜能和生物相容性的两种生理活性物质分别引入聚合物支架并与其表面共轭， 的制备。 由于智能多孔生物可降解聚合物支架由于引入能够有效诱导细胞增殖和分化至其表面和内部的生理活性物质而显示出改善的生物相容性和分化潜能，因此可直接诱导肌肉骨骼系统的原位组织再生 从移植聚合物支架和干细胞到人体中的生物体组织中的干细胞，而不需要额外的体外培养。 因此，根据本发明的智能多孔生物可降解聚合物支架可以有效地用于包括肌肉骨骼系统在内的各种组织和器官的再生。

公开(公告)号：US20100098762A1

公开(公告)日：2010-04-22

申请号：US12573037

申请日：2009-10-02

申请人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim

发明人： Dong Keun Han ,  Kwideok Park ,  Jae-Jin Kim

IPC分类号： A61K9/10 ,  A61K38/18 ,  A61K38/06 ,  A61K38/07 ,  A61K38/08 ,  A61P19/04

CPC分类号： A61K9/06 ,  A61K47/10 ,  A61K47/60

摘要： The present invention relates to a thermosensitive pluronic derivative hydrogel for tissue regeneration in which a biodegradable polymer is introduced at one end or both ends of a pluronic polymer, a methacryloxyethyl trimellitic acid anhydride is conjugated to the biodegradable polymer, and a physiologically active substance is fixed to the methacryloxyethyl trimellitic acid anhydride, as well as a method for the preparation thereof. The pluronic derivative hydrogel according to the present invention exhibits high biodegradability due to the introduction of a biodegradable polymer while still maintaining the themosensitivity of the pluronic polymer itself and shows good biocompatibility owing to the coupling with a physiologically active substance capable of improving cell adhesion, proliferation and differentiation. Therefore, the pluronic derivative hydrogel according to the present invention can be effectively used in the regeneration of various kinds of tissues and organs.

摘要翻译： 本发明涉及用于组织再生的热敏普朗尼克衍生物水凝胶，其中在聚醚聚合物的一端或两端引入可生物降解的聚合物，将甲基丙烯酰氧基乙基偏苯三酸酐与可生物降解的聚合物缀合，并且将生理活性物质固定 到甲基丙烯酰氧基乙基偏苯三酸酐，以及其制备方法。 本发明的聚醚衍生物水凝胶由于引入生物可降解聚合物而具有高生物降解性，同时仍然保持了普鲁隆聚合物本身的敏感性，并且由于与能够改善细胞粘附，增殖的生理活性物质的偶联而显示出良好的生物相容性 和分化。 因此，根据本发明的普流纶衍生物水凝胶可以有效地用于各种组织和器官的再生。

公开(公告)号：US09212259B2

公开(公告)日：2015-12-15

申请号：US13877384

申请日：2011-10-04

申请人： Won Jong Kim ,  Yanggy Lee ,  Kwideok Park ,  Jihoon Kim ,  Dong Keun Han

发明人： Won Jong Kim ,  Yanggy Lee ,  Kwideok Park ,  Jihoon Kim ,  Dong Keun Han

IPC分类号： A61K47/34 ,  A61L27/34 ,  C08L71/02 ,  C08G73/02 ,  A61L27/54 ,  C08G63/685

CPC分类号： C08G73/024 ,  A61K47/34 ,  A61L27/34 ,  A61L27/54 ,  A61L2300/114 ,  C08G63/6852 ,  C08G73/0206 ,  C08L71/02

摘要： A donor capable of controlled release of nitric monoxide and a prosthetic implant whose surface is coated with this donor are disclosed. The donor comprises a main chain of an organic polymer and a side chain that is covalently linked to the main chain and has a diazeniumdiolate functional group. The donor for controlled release of nitric monoxide exists in a fluid state such as liquid at room temperature and undergoes a sol-to-gel phase transition at a physiological pH as the temperature increases above the critical temperature. The critical temperature of the phase transition is in the range of 25 to 35° C., and the gel is a hydrogel without chemical cross-links.

摘要翻译： 公开了能够控制释放一氧化氮的供体和其表面涂覆有该供体的假体植入物。 供体包括有机聚合物的主链和共价连接到主链并具有二氮烯二硫酸酯官能团的侧链。 用于控制释放一氧化氮的供体以室温下的液体状态存在，并且随着温度升高到临界温度以上，在生理pH下经历溶胶 - 凝胶相变。 相变的临界温度在25至35℃的范围内，凝胶是没有化学交联的水凝胶。

公开(公告)号：US20130189340A1

公开(公告)日：2013-07-25

申请号：US13877384

申请日：2011-10-04

申请人： Won Jong Kim ,  Yanggy Lee ,  Kwideok Park ,  Jihoon Kim ,  Dong Keun Han

发明人： Won Jong Kim ,  Yanggy Lee ,  Kwideok Park ,  Jihoon Kim ,  Dong Keun Han

IPC分类号： C08G73/02 ,  A61L27/34

CPC分类号： C08G73/024 ,  A61K47/34 ,  A61L27/34 ,  A61L27/54 ,  A61L2300/114 ,  C08G63/6852 ,  C08G73/0206 ,  C08L71/02

摘要： A donor capable of controlled release of nitric monoxide and a prosthetic implant whose surface is coated with this donor are disclosed. The donor comprises a main chain of an organic polymer and a side chain that is covalently linked to the main chain and has a diazeniumdiolate functional group. The donor for controlled release of nitric monoxide exists in a fluid state such as liquid at room temperature and undergoes a sol-to-gel phase transition at a physiological pH as the temperature increases above the critical temperature. The critical temperature of the phase transition is in the range of 25 to 35° C., and the gel is a hydrogel without chemical cross-links.

摘要翻译： 公开了能够控制释放一氧化氮的供体和其表面涂覆有该供体的假体植入物。 供体包括有机聚合物的主链和共价连接到主链并具有二氮烯二硫酸酯官能团的侧链。 用于控制释放一氧化氮的供体以室温下的液体状态存在，并且随着温度升高到临界温度以上，在生理pH下经历溶胶 - 凝胶相变。 相变的临界温度在25至35℃的范围内，凝胶是没有化学交联的水凝胶。

公开(公告)号：US07842305B2

公开(公告)日：2010-11-30

申请号：US11526280

申请日：2006-09-21

申请人： Dong Keun Han ,  Kwang-Duk Ahn ,  Hyun Jung Jung ,  Kwideok Park

发明人： Dong Keun Han ,  Kwang-Duk Ahn ,  Hyun Jung Jung ,  Kwideok Park

IPC分类号： A61F2/00

CPC分类号： A61L27/56 ,  A61L27/18 ,  A61L27/58 ,  C08L67/04

摘要： There is provided a method of preparing biodegradable dual pore polymer scaffolds, comprising the steps of: maintaining a polymer solution containing a biodegradable polymer, an effervescent mixture of carbonate and organic acid, and solvent at a temperature range of −196° C. to ambient temperature so as to evaporate the solvent and produce a polymer sample; and foaming the polymer sample in a mixed solution of water and alcohol.According to the present invention, the pore size of the polymer scaffolds can be easily controlled, and biodegradable polymer scaffolds can be more simply prepared compared to conventional methods such as salt leaching technique, phase separation technique or gas foaming technique. Further, since the biodegradable polymer scaffolds prepared according to the present invention have a high surface area and porosity without formation of skin layer, secretion of toxic substances and remnant phenomenon, they can be advantageously used for regenerating almost all living tissues and organs in tissue engineering.

摘要翻译： 提供了一种制备可生物降解的双孔聚合物支架的方法，包括以下步骤：将含有可生物降解的聚合物，碳酸盐和有机酸的泡腾混合物和溶剂的聚合物溶液在-196℃的温度范围内保持至环境温度 温度以蒸发溶剂并产生聚合物样品; 并将聚合物样品在水和酒精的混合溶液中发泡。 根据本发明，与常规方法如盐浸技术，相分离技术或气体发泡技术相比，可以容易地控制聚合物支架的孔径，并且可以更简单地制备可生物降解的聚合物支架。 此外，由于根据本发明制备的可生物降解的聚合物支架具有高的表面积和孔隙率，而没有形成皮肤层，有毒物质的分泌和残留现象，因此它们可有利地用于组织工程中几乎所有活组织和器官的再生 。

公开(公告)号：US20100136645A1

公开(公告)日：2010-06-03

申请号：US12596008

申请日：2007-04-17

申请人： Byoung-Hyun Min ,  So Ra Park ,  Cheng Zhe Jin ,  Kwideok Park

发明人： Byoung-Hyun Min ,  So Ra Park ,  Cheng Zhe Jin ,  Kwideok Park

IPC分类号： C12N13/00 ,  C12N5/02

CPC分类号： C12N5/0655 ,  A61L27/3633 ,  A61L27/3817 ,  A61L27/48 ,  A61L27/56 ,  C12N2533/54 ,  C12N2533/90 ,  C08L67/04

摘要： The present invention relates to a method for fabricating a cell-derived extracellular matrix scaffold, more particularly, to a method for fabricating a cell-derived extracellular matrix scaffold, the method comprising the steps of obtaining a chondrocyte/extracellular matrix (ECM) membrane from chondrocytes derived from animal cartilage, obtaining a pellet-type scaffold-free construct by culturing after centrifuging the obtained chondrocytes/extracellular matrix (ECM) membrane and freeze-drying the obtained pellet-type construct. The cell-derived ECM scaffold according to the invention is a porous scaffold fabricated using cartilage tissue engineered by culturing chondrocytes in an in vitro scaffold-free system, which is not reduced in size during the cultivation and thus useful for cartilage regeneration.

摘要翻译： 本发明涉及一种用于制备细胞衍生的细胞外基质支架的方法，更具体地涉及一种制备细胞衍生的细胞外基质支架的方法，所述方法包括以下步骤：从软骨细胞/细胞外基质（ECM） 从动物软骨衍生的软骨细胞，通过在离心获得的软骨细胞/细胞外基质（ECM）膜并冷冻干燥获得的颗粒型构建体之后培养获得颗粒型无支架构建体。 根据本发明的细胞衍生的ECM支架是使用在体外无支架体系中培养软骨细胞工程化的软骨组织制造的多孔支架，其在培养期间尺寸不减小，因此可用于软骨再生。

公开(公告)号：US20070092557A1

公开(公告)日：2007-04-26

申请号：US11526280

申请日：2006-09-21

申请人： Dong Han ,  Kwang-Duk Ahn ,  Hyun Jung ,  Kwideok Park

发明人： Dong Han ,  Kwang-Duk Ahn ,  Hyun Jung ,  Kwideok Park

IPC分类号： A61K9/70 ,  B29C65/00

CPC分类号： A61L27/56 ,  A61L27/18 ,  A61L27/58 ,  C08L67/04

摘要： There is provided a method of preparing biodegradable dual pore polymer scaffolds, comprising the steps of: maintaining a polymer solution containing a biodegradable polymer, an effervescent mixture of carbonate and organic acid, and solvent at a temperature range of −196° C. to ambient temperature so as to evaporate the solvent and produce a polymer sample; and foaming the polymer sample in a mixed solution of water and alcohol. According to the present invention, the pore size of the polymer scaffolds can be easily controlled, and biodegradable polymer scaffolds can be more simply prepared compared to conventional methods such as salt leaching technique, phase separation technique or gas foaming technique. Further, since the biodegradable polymer scaffolds prepared according to the present invention have a high surface area and porosity without formation of skin layer, secretion of toxic substances and remnant phenomenon, they can be advantageously used for regenerating almost all living tissues and organs in tissue engineering.

摘要翻译： 提供了一种制备可生物降解的双孔聚合物支架的方法，包括以下步骤：将含有可生物降解的聚合物，碳酸盐和有机酸的泡腾混合物和溶剂的聚合物溶液保持在-196℃至环境温度 温度以蒸发溶剂并产生聚合物样品; 并将聚合物样品在水和酒精的混合溶液中发泡。 根据本发明，与常规方法如盐浸技术，相分离技术或气体发泡技术相比，可以容易地控制聚合物支架的孔径，并且可以更简单地制备可生物降解的聚合物支架。 此外，由于根据本发明制备的可生物降解的聚合物支架具有高的表面积和孔隙率，而没有形成皮肤层，有毒物质的分泌和残留现象，因此它们可有利地用于组织工程中几乎所有活组织和器官的再生 。