公开(公告)号：US20210285046A1

公开(公告)日：2021-09-16

申请号：US17220534

申请日：2021-04-01

Applicant: 10x Genomics, Inc. ,  Spatial Transcriptomics AB

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20210214785A1

公开(公告)日：2021-07-15

申请号：US17147874

申请日：2021-01-13

Applicant: Spatial Transcriptomics AB

Inventor： Marlon Stoeckius

IPC: C12Q1/6874 ,  C12Q1/6837

Abstract: Provided herein are methods of determining a location of a target analyte in a non-permeabilized biological sample and methods of reducing background binding of an analyte on an array.

公开(公告)号：US20210223227A1

公开(公告)日：2021-07-22

申请号：US17150997

申请日：2021-01-15

Applicant: Spatial Transcriptomics AB

Inventor： Marlon Stoeckius

IPC: G01N33/487 ,  G01N27/30 ,  G01N27/404

Abstract: An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.

公开(公告)号：US20250122564A1

公开(公告)日：2025-04-17

申请号：US18980743

申请日：2024-12-13

Applicant: 10x Genomics, Inc.

Inventor： Marco Mignardi ,  Marlon Stoeckius

IPC: C12Q1/6874

Abstract: Provided herein are methods of identifying abundance and location of an RNA in a biological sample using an adaptor sequence and a primer. Also disclosed herein are kits. compositions, and systems that are used to perform the methods.

公开(公告)号：US20240417783A1

公开(公告)日：2024-12-19

申请号：US18785905

申请日：2024-07-26

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Marlon Stoeckius

IPC: C12Q1/6837 ,  C12Q1/48 ,  G01N1/30

Abstract: Provided herein are methods and kits for binding of an analyte capture sequence to a capture domain of a capture probe.

公开(公告)号：US20240043925A1

公开(公告)日：2024-02-08

申请号：US18490390

申请日：2023-10-19

Applicant: 10x Genomics, Inc.

Inventor： Zachary Bent ,  Marlon Stoeckius

IPC: C12Q1/6876

CPC classification number: C12Q1/6876

Abstract: Provided herein are methods of detecting target nucleic acids and uses of the same.

公开(公告)号：US20230383344A1

公开(公告)日：2023-11-30

申请号：US18448618

申请日：2023-08-11

Applicant: 10x Genomics, Inc.

Inventor： Marlon Stoeckius

IPC: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/48 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/485 ,  C12Q1/6806 ,  G01N2333/9015 ,  G01N2333/9126 ,  G01N2333/922 ,  C12Y301/26004 ,  C12Y207/07007 ,  C12Y605/01001

Abstract: Provided herein are methods of identifying a location of an RNA in a sample that include: (a) contacting the sample with an array comprising capture probes, where a capture probe comprises a capture domain and a spatial barcode; (b) releasing the RNA from the sample; (c) extending a 3′ end of the capture probe using the capture domain-bound RNA as a template; (d) generating nick(s) in the extended capture probe-hybridized RNA and performing random-primed DNA synthesis; (e) performing end repair on the second strand DNA molecule; (f) adding a single adenosine nucleotide to the 3′ end of the extended capture probe; (g) ligating a double-stranded sequencing adaptor to the double-stranded DNA product; and (h) determining all or a part of the sequence of the RNA, and the sequence of the spatial barcode, or complements thereof, and using the determined sequences to identify the location of the RNA in the sample.

公开(公告)号：US20230183684A1

公开(公告)日：2023-06-15

申请号：US18165721

申请日：2023-02-07

Applicant: 10x Genomics, Inc.

Inventor： Caroline Julie Gallant ,  Marlon Stoeckius ,  Katherine Pfeiffer

IPC: C12N15/10 ,  C12Q1/6837 ,  C12Q1/6874

CPC classification number: C12N15/1093 ,  C12Q1/6837 ,  C12Q1/6874

Abstract: Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

公开(公告)号：US11332790B2

公开(公告)日：2022-05-17

申请号：US17220529

申请日：2021-04-01

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20220025447A1

公开(公告)日：2022-01-27

申请号：US17312238

申请日：2019-12-06

Applicant: 10x Genomics, Inc.

Inventor： Augusto Manuel Tentori ,  Marlon Stoeckius

IPC: C12Q1/6837 ,  C12Q1/6841

Abstract: The present disclosure relates to materials and methods for generating spatial arrays with gradients and using the generated spatial arrays to identify the location of analytes present in a biological sample.