公开(公告)号：US20210214785A1

公开(公告)日：2021-07-15

申请号：US17147874

申请日：2021-01-13

Applicant: Spatial Transcriptomics AB

Inventor： Marlon Stoeckius

IPC: C12Q1/6874 ,  C12Q1/6837

Abstract: Provided herein are methods of determining a location of a target analyte in a non-permeabilized biological sample and methods of reducing background binding of an analyte on an array.

公开(公告)号：US20210285046A1

公开(公告)日：2021-09-16

申请号：US17220534

申请日：2021-04-01

Applicant: 10x Genomics, Inc. ,  Spatial Transcriptomics AB

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20210223227A1

公开(公告)日：2021-07-22

申请号：US17150997

申请日：2021-01-15

Applicant: Spatial Transcriptomics AB

Inventor： Marlon Stoeckius

IPC: G01N33/487 ,  G01N27/30 ,  G01N27/404

Abstract: An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.