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    COMPOSITIONS AND METHODS FOR ENHANCING PRODUCTION OF A BIOLOGICAL PRODUCT
    1.
    发明申请
    COMPOSITIONS AND METHODS FOR ENHANCING PRODUCTION OF A BIOLOGICAL PRODUCT 审中-公开
    用于增强生物产品生产的组合物和方法

    公开(公告)号：US20140099666A1

    公开(公告)日：2014-04-10

    申请号：US13379511

    申请日：2010-07-06

    申请人： Anthony Rossomando ,  John M. Maraganore ,  Stuart Pollard ,  David Kocisko ,  Muthiah Manoharan ,  Gregory Hinkle ,  Brian Bettencourt ,  Shannon Hogan

    发明人： Anthony Rossomando ,  John M. Maraganore ,  Stuart Pollard ,  David Kocisko ,  Muthiah Manoharan ,  Gregory Hinkle ,  Brian Bettencourt ,  Shannon Hogan

    IPC分类号： C07K16/00

    CPC分类号： C07K16/00 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/3515

    摘要： The invention provides compositions and methods for producing a biological product from a host cell. In various embodiments, the biological product is a polypeptide, a metabolite, a nutraceutical, a chemical intermediate, a biofuel, a food additive, or an antibiotic. In one aspect, the invention provides for a method for producing a biological product from a host cell. The method generally comprises contacting the cell with a RNA effector molecule, a portion of which is complementary to a target gene, maintaining the cell in a large-scale bioreactor for a time sufficient to modulate expression of the target gene, wherein the modulation enhances production of the biological product from the cell, and isolating the biological product from the cell.

    摘要翻译： 本发明提供了用于从宿主细胞生产生物制品的组合物和方法。 在各种实施方案中，生物产物是多肽，代谢物，营养保健品，化学中间体，生物燃料，食品添加剂或抗生素。 一方面，本发明提供从宿主细胞产生生物制品的方法。 该方法通常包括使细胞与RNA效应分子接触，其一部分与靶基因互补，将细胞维持在大规模生物反应器中足以调节靶基因表达的时间，其中调节增强生产 的来自细胞的生物产物，并从细胞中分离生物产物。

    BIOPROCESSING
    2.
    发明申请
    BIOPROCESSING 审中-公开
    生物学

    公开(公告)号：US20140154783A1

    公开(公告)日：2014-06-05

    申请号：US13379797

    申请日：2010-07-06

    申请人： Anthony Rossomando ,  John M. Maraganore ,  Stuart Pollard ,  David Kocisko ,  Muthiah Manogaran ,  Todd Borland ,  Shannon Hogan

    发明人： Anthony Rossomando ,  John M. Maraganore ,  Stuart Pollard ,  David Kocisko ,  Muthiah Manogaran ,  Todd Borland ,  Shannon Hogan

    IPC分类号： C12N7/00

    CPC分类号： C12N7/00 ,  C12N15/113 ,  C12N15/1131 ,  C12N2310/14

    摘要： The present invention relates to molecular biology, molecular genetics, and bioprocessing. The embodiments provide for compositions and methods for producing a biological product, such as an immunogenic agent, in an embryonated egg by introducing into the egg a RNA effector molecule capable of modulating expression of a target gene, wherein the modulation enhances production of the biological product in the egg. These methods provide for RNAi-based approaches to optimize the production of biologics from embryonated eggs, such as the production of viral vaccines including seasonal and pandemic flu vaccines. The invention also relates to molecules, reagents, cells, and kits useful for carrying out the methods, and biological products produced by the methods.

    摘要翻译： 本发明涉及分子生物学，分子遗传学和生物处理。 这些实施方案提供了通过向蛋中引入能够调节靶基因表达的RNA效应分子来生产在胚胎卵中的生物产物如免疫原性试剂的组合物和方法，其中所述调节增强了生物产物的产生 在蛋里 这些方法提供了基于RNAi的方法来优化来自胚胎卵的生物制品的生产，例如生产包括季节性和大流行性流感疫苗的病毒疫苗。 本发明还涉及可用于实施方法的分子，试剂，细胞和试剂盒，以及通过该方法生产的生物制品。

    GENE AMPLIFICATION AND TRANSFECTION METHODS AND REAGENTS RELATED THERETO
    3.
    发明申请
    GENE AMPLIFICATION AND TRANSFECTION METHODS AND REAGENTS RELATED THERETO 审中-公开
    基因扩增和转运方法和相关试剂

    公开(公告)号：US20130164851A1

    公开(公告)日：2013-06-27

    申请号：US13636379

    申请日：2011-03-25

    申请人： Anthony Rossomando ,  Gregory P. Thill ,  Stuart Pollard

    发明人： Anthony Rossomando ,  Gregory P. Thill ,  Stuart Pollard

    IPC分类号： C12N15/85

    CPC分类号： C12N15/1137 ,  C12N15/1138 ,  C12N2310/14

    摘要： Provided herein are methods and compositions for generating a cell line capable of producing a biological product, using a gene amplification based system. Methods and compositions are provided to inhibit endogenous selectable amplifiable marker genes using RNA interference and prevent the selection of false positives during generation of a custom cell line. Such methods improve efficiency of cell line development and do not require the use of specialized substrates or cells lacking the endogenous selectable amplifiable marker gene to negate the effect of endogenously expressed levels of the selectable amplifiable marker gene in cells.

    摘要翻译： 本文提供了使用基于基因扩增的系统产生能够产生生物产物的细胞系的方法和组合物。 提供方法和组合物以使用RNA干扰来抑制内源性选择性可扩增标记基因，并防止在产生定制细胞系期间选择假阳性。 这样的方法提高了细胞系发育的效率，并且不需要使用缺乏内源可选择可扩增标记基因的特异性底物或细胞来消除细胞内可选择可扩增标记基因的内源表达水平的作用。

    Multi-axis air/electrical control system
    4.
    发明授权
    Multi-axis air/electrical control system 失效
    多轴空气/电气控制系统

    公开(公告)号：US06989646B2

    公开(公告)日：2006-01-24

    申请号：US10422743

    申请日：2003-04-24

    申请人： Stuart Pollard Jackson ,  Marlin Harold Thompson ,  Earl Christian Close ,  Larry Patrick Munger ,  Jeffrey William Fenner ,  Ted Anthony Reed

    发明人： Stuart Pollard Jackson ,  Marlin Harold Thompson ,  Earl Christian Close ,  Larry Patrick Munger ,  Jeffrey William Fenner ,  Ted Anthony Reed

    IPC分类号： G05D16/00

    CPC分类号： F15B9/12 ,  Y10T74/18592

    摘要： A system for allowing a wide selection of components to affect a desired result in the positioning business. Up to nine axes of coordinated motion are offered each of which may use a servomotor, a single or double rod air cylinder. The control for the system is a series of digital commands that cause the cylinder to accelerate, decelerate, maintain a given velocity, stop or pause and repeat a series of commands. It uses a low power servo system which, when activated, opens an air valve to an air cylinder which then moves until the valve actuator is turned off by the cylinder thus causing the air cylinder to follow the lower power servo system.

    摘要翻译： 一种允许广泛选择的组件影响定位业务所需结果的系统。 提供多达九个协调运动的轴，每个轴可以使用伺服电机，单杆或双杆气缸。 系统的控制是一系列数字命令，使得气缸加速，减速，保持给定的速度，停止或暂停并重复一系列命令。 它使用低功率伺服系统，其在被激活时将空气阀打开到气缸，然后气缸移动直到阀致动器被气缸关闭，从而使气缸跟随下功率伺服系统。

    CELL-BASED BIOPROCESSING
    5.
    发明申请
    CELL-BASED BIOPROCESSING 审中-公开
    基于细胞的生物学方法

    公开(公告)号：US20140004565A1

    公开(公告)日：2014-01-02

    申请号：US13380182

    申请日：2010-07-06

    申请人： Anthony Rossomando ,  Muthiah Manoharan ,  Gregory Hinkle ,  Brian Bettencourt ,  John M. Maraganora ,  Stuart Pollard ,  David Kocisko ,  Shannon Hogan

    发明人： Anthony Rossomando ,  Muthiah Manoharan ,  Gregory Hinkle ,  Brian Bettencourt ,  John M. Maraganora ,  Stuart Pollard ,  David Kocisko ,  Shannon Hogan

    IPC分类号： C07K16/28

    CPC分类号： C07K16/2887 ,  C12N15/1135 ,  C12N15/1137 ,  C12N2310/14 ,  C12Y101/01027

    摘要： The invention provides compositions and methods for producing an immunogenic agent from a host cell. In various embodiments, the immunogenic agent is a polypeptide, an antigen, a virus particle, or a vaccine In one aspect, the invention provides for a method for producing an immunogenic agent from a host cell. The method generally comprises contacting the cell with a RNA effector molecule, a portion of which is complementary to a target gene, maintaining the cell in a large-scale bioreactor for a time sufficient to modulate expression of the target gene, wherein the modulation enhances production of the immunogenic agent from the cell, and isolating the immunogenic agent from the cell.

    摘要翻译： 本发明提供了用于从宿主细胞产生免疫原性试剂的组合物和方法。 在各种实施方案中，免疫原性试剂是多肽，抗原，病毒颗粒或疫苗。一方面，本发明提供了用于从宿主细胞产生免疫原性试剂的方法。 该方法通常包括使细胞与RNA效应分子接触，其一部分与靶基因互补，将细胞维持在大规模生物反应器中足以调节靶基因表达的时间，其中调节增强生产 的免疫原性试剂，并从细胞中分离免疫原性试剂。