公开(公告)号：US20130164329A1

公开(公告)日：2013-06-27

申请号：US13578886

申请日：2011-02-16

Applicant: Anthony Rossomando ,  John M. Maraganore ,  Stuart Pollard ,  Muthiah Manoharan

Inventor： Anthony Rossomando ,  John M. Maraganore ,  Stuart Pollard ,  Muthiah Manoharan

IPC: C12N7/02 ,  C12N7/00 ,  G01N33/569 ,  C12N1/20 ,  C12N1/10 ,  C12Q1/70 ,  A61K39/15

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/15 ,  C12N2720/12334 ,  C12N2720/12352 ,  C12N2750/10052 ,  C12Q1/70

Abstract: The present invention relates to compositions and methods for isolating cells devoid of unwanted viral contaminants, and to methods for preparing a virus stock substantially devoid of viral contaminants. Virus stocks, cells, and immunogenic reagents produced using such methods are also provided.

Abstract translation: 本发明涉及用于分离没有不需要的病毒污染物的细胞的组合物和方法，以及用于制备基本上不含病毒污染物的病毒原料的方法。 还提供了使用这些方法生产的病毒库，细胞和免疫原性试剂。

公开(公告)号：US20240200099A1

公开(公告)日：2024-06-20

申请号：US18555035

申请日：2022-04-11

Applicant: Merck Sharp & Dohme LLC

Inventor： Dai Wang ,  Jiajie Wei

IPC: C12N15/86

CPC classification number: C12N15/86 ,  C12N2720/12322 ,  C12N2720/12343 ,  C12N2720/12352

Abstract: Rotavirus vectors encoding in their genome a heterologous gene, and nucleic acid constructs encoding such rotavirus vectors. The rotavirus vector genome may include a rotavirus non-structural protein, a 2A peptide downstream of the rotavirus non-structural protein, and a heterologous protein downstream of the 2A peptide. The heterologous gene may be, for example, a SARS-CoV-2 spike protein or a fragment thereof, or an RSV F protein or a fragment thereof.

公开(公告)号：US20240309336A1

公开(公告)日：2024-09-19

申请号：US18576816

申请日：2022-06-24

Applicant: SK BIOSCIENCE CO., LTD.

Inventor： Ki Weon SEO ,  Tae Woo KWON ,  Seo Yeon JUNG ,  Min Jung PARK ,  Kun Se LEE ,  Hyun Joo LEE

IPC: C12N7/00 ,  C12N15/10

CPC classification number: C12N7/00 ,  C12N15/1086 ,  C12N2720/12352

Abstract: The present application relates to a method for producing a reassortant Reoviridae virus and a vector library for the same, and provides: a method for producing a reassortant Reoviridae virus by using a cell line into which an RNA polymerase is introduced and an expression vector library according to an aspect; a reassortant Reoviridae virus produced by the method; and an expression vector library for producing reassortant rotavirus.

公开(公告)号：US20150252334A1

公开(公告)日：2015-09-10

申请号：US14429586

申请日：2013-06-24

Applicant: XIAMEN UNIVERSITY ,  XIAMEN INNOVAX BIOTECH CO., LTD.

Inventor： Shengxiang Ge ,  Tingdong Li ,  Qingshun Guo ,  Feihai Xu ,  Jun Zhang ,  Ningshao Xia

IPC: C12N7/00

CPC classification number: C12N7/00 ,  A61K38/162 ,  A61K39/00 ,  C12N7/04 ,  C12N2720/12322 ,  C12N2720/12323 ,  C12N2720/12333 ,  C12N2720/12334 ,  C12N2720/12352

Abstract: The invention relates to a method for preparing double-layered virus-like particles of rotavirus in vitro. The method comprises the following steps: purifying rotavirus VP6 proteins from a lysis supernatant, and in vitro assembling double-layered virus-like particles consisting of VP2 proteins and VP6 proteins, wherein the proteins and the virus-like particles can be used for preventing or reducing the clinical symptoms caused by rotavirus infection.

Abstract translation: 本发明涉及一种体外制备轮状病毒双层病毒样颗粒的方法。 该方法包括以下步骤：从裂解上清液中纯化轮状病毒VP6蛋白，以及体外组装由VP2蛋白和VP6蛋白组成的双层病毒样颗粒，其中蛋白质和病毒样颗粒可用于预防或 减少轮状病毒感染引起的临床症状。

公开(公告)号：US11952400B2

公开(公告)日：2024-04-09

申请号：US17232952

申请日：2021-04-16

Applicant: TECON BIOPHARMACEUTICAL CO., LTD.

Inventor： Sun He ,  Yiping Pan ,  Guoqing Zhang ,  Pengxian Yan ,  Na Xi ,  Miaomiao Guo ,  Shengdong Xiao ,  Tianzeng Li ,  Rui Han ,  Yumeng Wang ,  Jiubin Du ,  Pei Zheng ,  Jian Cao

IPC: C07K14/005 ,  A61K39/108 ,  A61K39/15 ,  C07K14/245 ,  C12N7/00 ,  A61K39/00

CPC classification number: C07K14/005 ,  A61K39/0258 ,  A61K39/15 ,  C07K14/245 ,  C12N7/00 ,  A61K2039/5258 ,  A61K2039/552 ,  A61K2039/70 ,  C07K2319/40 ,  C12N2720/12322 ,  C12N2720/12323 ,  C12N2720/12334 ,  C12N2720/12352 ,  C12N2770/20022 ,  C12N2770/20023 ,  C12N2770/20034 ,  C12N2770/20052

Abstract: Provided are a bovine rotavirus fusion protein and calf diarrhea multivalent vaccine. The bovine rotavirus fusion protein contains a VP6 fragment, wherein the VP6 fragment contains an amino acid sequence as represented by SEQ ID NO. 4, and at least one loop region of the following (a)˜(c) is substituted with an antigenic epitope derived from bovine coronavirus and/or an antigenic epitope derived from E. coli: (a) amino acid residues of sites 168-177; with an amino acid sequence as represented by SEQ ID NO. 1; (b) amino acid residues of sites 194-205; with an amino acid sequence as represented by SEQ ID NO. 2; and (a) amino acid residues of sites 296-316, with an amino acid sequence as represented by SEQ ID NO. 3, The bovine rotavirus fusion protein contains a plurality of antigenic epitopes, and can enable a host to generate a plurality of antibodies after immunizing the host.

公开(公告)号：US09862933B2

公开(公告)日：2018-01-09

申请号：US14429586

申请日：2013-06-24

Applicant: Xiamen University ,  Xiamen Innovax Biotech Co., Ltd.

Inventor： Shengxiang Ge ,  Tingdong Li ,  Qingshun Guo ,  Feihai Xu ,  Jun Zhang ,  Ningshao Xia

IPC: C12N7/04 ,  C12N7/00 ,  A61K38/16 ,  A61K39/00

CPC classification number: C12N7/00 ,  A61K38/162 ,  A61K39/00 ,  C12N7/04 ,  C12N2720/12322 ,  C12N2720/12323 ,  C12N2720/12333 ,  C12N2720/12334 ,  C12N2720/12352

Abstract: The invention relates to a method for preparing double-layered virus-like particles of rotavirus in vitro. The method comprises the following steps: purifying rotavirus VP6 proteins from a lysis supernatant, and in vitro assembling double-layered virus-like particles consisting of VP2 proteins and VP6 proteins, wherein the proteins and the virus-like particles can be used for preventing or reducing the clinical symptoms caused by rotavirus infection.