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公开(公告)号：US08691951B2
公开(公告)日：2014-04-08
申请号：US13027088
申请日：2011-02-14
申请人： Shunji Hattori , Yoh-ichi Koyama , Hitomi Fujisaki , Kiyotoshi Sekiguchi , Sugiko Futaki , Ryoko Sato
发明人： Shunji Hattori , Yoh-ichi Koyama , Hitomi Fujisaki , Kiyotoshi Sekiguchi , Sugiko Futaki , Ryoko Sato
IPC分类号： A61K38/17 , A61K47/48 , C07K1/00 , C07K14/00 , C07K16/00 , C07K17/00 , C12M1/22 , C12M3/00 , A61K38/39
摘要： It is an object of the present invention to provide a Type I-Type IV collagen hybrid gel, which maintains characteristics of a Type IV collagen and is superior in gel strength. It is the Type I-Type IV collagen hybrid gel obtained by mixing 100 to 500 parts by mass of the Type I collagen having fibrosis ability, relative to 100 parts by mass of the Type IV collagen having gelling ability. A three-dimensional structure is formed, where a membrane-like substance by the Type IV collagen is formed onto a fibrous substance by the Type I collagen, so as to be able to provide cell culture environment approximate to a basement membrane of a living body.
摘要翻译： 本发明的目的是提供一种IV型胶原蛋白混合凝胶，其保持IV型胶原的特性并且凝胶强度优异。 相对于100质量份具有胶凝能力的IV型胶原，将100〜500质量份具有纤维化能力的I型胶原混合，得到I型IV型胶原混合凝胶。 形成三维结构，其中通过I型胶原将IV型胶原的膜状物质形成在纤维状物质上，以便能够提供接近生物体基底膜的细胞培养环境 。
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公开(公告)号：US20120220031A1
公开(公告)日：2012-08-30
申请号：US13500452
申请日：2010-10-07
申请人： Kiyotoshi Sekiguchi , Sugiko Futaki , Yukimasa Taniguchi , Maria Hayashi , Norio Nakatsuji , Takamichi Miyazaki , Eihachiro Kawase , Hirofumi Suemori
发明人： Kiyotoshi Sekiguchi , Sugiko Futaki , Yukimasa Taniguchi , Maria Hayashi , Norio Nakatsuji , Takamichi Miyazaki , Eihachiro Kawase , Hirofumi Suemori
IPC分类号： C12N5/071
CPC分类号： C12N5/0606 , C12N5/0696 , C12N2533/52
摘要： The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4×104 to 10×104 cells/cm2 onto a culture substrate coated with human laminin α5β1γ1 E8 fragment or human laminin α3β3γ2 E8 fragment preferably at a concentration of 0.5 to 25 μg/cm2, the human pluripotent stem cells can be rapidly expanded in a pluripotent state.
摘要翻译： 本发明提供一种培养基质，其能够在无饲养细胞培养环境下使多能干细胞维持培养，使用培养基质培养人多能干细胞的培养方法。 通过将以4×10 4〜10×10 4个细胞/ cm 2的细胞密度分离为单个细胞的人多能干细胞接种到涂有人层粘连蛋白α5和bgr1Y1E8片段或人层粘连蛋白α3和bgr3Y3E8片段的培养基上，优选浓度 0.5至25μg/ cm 2，人多能干细胞可以在多能状态下快速扩张。
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公开(公告)号：US20120208761A1
公开(公告)日：2012-08-16
申请号：US13027088
申请日：2011-02-14
申请人： Shunji Hattori , Yoh-ichi Koyama , Hitomi Fujisaki , Kiyotoshi Sekiguchi , Sugiko Futaki , Ryoko Sato
发明人： Shunji Hattori , Yoh-ichi Koyama , Hitomi Fujisaki , Kiyotoshi Sekiguchi , Sugiko Futaki , Ryoko Sato
摘要： It is an object of the present invention to provide a Type I-Type IV collagen hybrid gel, which maintains characteristics of a Type IV collagen and is superior in gel strength. It is the Type I-Type IV collagen hybrid gel obtained by mixing 100 to 500 parts by mass of the Type I collagen having fibrosis ability, relative to 100 parts by mass of the Type IV collagen having gelling ability. A three-dimensional structure is formed, where a membrane-like substance by the Type IV collagen is formed onto a fibrous substance by the Type I collagen, so as to be able to provide cell culture environment approximate to a basement membrane of a living body.
摘要翻译： 本发明的目的是提供一种IV型胶原蛋白混合凝胶，其保持IV型胶原的特性并且凝胶强度优异。 相对于100质量份具有胶凝能力的IV型胶原，将100〜500质量份具有纤维化能力的I型胶原混合，得到I型IV型胶原混合凝胶。 形成三维结构，其中通过I型胶原将IV型胶原的膜状物质形成在纤维状物质上，以便能够提供接近生物体基底膜的细胞培养环境 。
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公开(公告)号：US08877493B2
公开(公告)日：2014-11-04
申请号：US13500452
申请日：2010-10-07
申请人： Kiyotoshi Sekiguchi , Sugiko Futaki , Yukimasa Taniguchi , Maria Hayashi , Norio Nakatsuji , Takamichi Miyazaki , Eihachiro Kawase , Hirofumi Suemori
发明人： Kiyotoshi Sekiguchi , Sugiko Futaki , Yukimasa Taniguchi , Maria Hayashi , Norio Nakatsuji , Takamichi Miyazaki , Eihachiro Kawase , Hirofumi Suemori
IPC分类号： C12N5/071 , C12N5/00 , C12N5/074 , C12N5/0735
CPC分类号： C12N5/0606 , C12N5/0696 , C12N2533/52
摘要： The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4×104 to 10×104 cells/cm2 onto a culture substrate coated with human laminin α5β1γ1 E8 fragment or human laminin α3β3γ2 E8 fragment preferably at a concentration of 0.5 to 25 μg/cm2, the human pluripotent stem cells can be rapidly expanded in a pluripotent state.
摘要翻译： 本发明提供一种培养基质，其能够在无饲养细胞培养环境下使多能干细胞维持培养，使用培养基质培养人多能干细胞的培养方法。 通过将以4×10 4〜10×10 4个细胞/ cm 2的细胞密度分离为单个细胞的人多能干细胞接种到涂有人层粘连蛋白α5和bgr1Y1E8片段或人层粘连蛋白α3和bgr3Y3E8片段的培养基上，优选浓度 0.5至25μg/ cm 2，人多能干细胞可以在多能状态下快速扩张。
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