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    Culture Medium for Culturing Feeder Cells for Embryonic Stem Cells Culture and the Prepared Feeder Cells
    3.
    发明申请
    Culture Medium for Culturing Feeder Cells for Embryonic Stem Cells Culture and the Prepared Feeder Cells 审中-公开
    用于培养胚胎干细胞培养和准备的饲养细胞的培养基的培养基

    公开(公告)号：US20080085554A1

    公开(公告)日：2008-04-10

    申请号：US10588804

    申请日：2005-02-10

    申请人： Norio Nakatsuji ,  Hirofumi Suemori ,  Isao Asaka ,  Harafumi Sugai ,  Reiko Okamoto

    发明人： Norio Nakatsuji ,  Hirofumi Suemori ,  Isao Asaka ,  Harafumi Sugai ,  Reiko Okamoto

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0652 ,  C12N5/0606 ,  C12N2500/44 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2533/54

    摘要： A culture medium for preparation of feeder cells for embryonic stem cells, which can efficiently establish feeder cells for use in culture of embryonic stem cells including human's from limited donor-derived materials and culture them in a condition of a reduced risk of infection, is provided. Further, a preparation method of feeder cells, which is relatively safe even when subjected to coculture with embryonic stem cells including human's, and the resulting feeder cells therefrom are provided. With the culture medium for preparation of feeder cells for embryonic stem cells comprising at least a serum albumin and insulin in a basal medium, a cell population comprising at least one kind of cells selected from fetal skin fibroblasts, fetal myofibroblasts, fetal lung fibroblasts, fetal epithelial cells, fetal endothelial cells, adult skin fibroblasts, adult lung fibroblasts, adult epithelial cells and endothelial cells which can become feeder cells for embryonic stem cells can be stably proliferated.

    摘要翻译： 提供用于胚胎干细胞的饲养细胞的制备的培养基，其可以有效地建立饲养细胞用于胚胎干细胞的培养，所述饲养细胞包括来自有限供体来源的材料的人的胚胎干细胞的培养，并且在感染风险降低的条件下培养它们。 。 此外，提供了饲养细胞的制备方法，即使在与包括人的胚胎干细胞进行共培养的情况下也是相对安全的，以及所得到的饲养细胞。 使用包含至少一种选自胎儿皮肤成纤维细胞，胎儿肌成纤维细胞，胎儿肺成纤维细胞，胎儿成纤维细胞，胎儿成纤维细胞，胎儿成纤维细胞，胎儿成纤维细胞， 可以稳定地增殖上皮细胞，胎儿内皮细胞，成年皮肤成纤维细胞，成年肺成纤维细胞，成年上皮细胞和成为胚胎干细胞的饲养细胞的内皮细胞。

    Cryopreservation medium for primate embryo stem cells and cryopreservation method
    4.
    发明申请
    Cryopreservation medium for primate embryo stem cells and cryopreservation method 审中-公开
    用于灵长类动物胚胎干细胞的冷冻保存培养基和冷冻保存方法

    公开(公告)号：US20050026133A1

    公开(公告)日：2005-02-03

    申请号：US10902571

    申请日：2004-07-30

    申请人： Norio Nakatsuji ,  Hirofumi Suemori ,  Isao Asaka

    发明人： Norio Nakatsuji ,  Hirofumi Suemori ,  Isao Asaka

    IPC分类号： A01N1/02 ,  C12N5/02 ,  C12N5/0735 ,  C12N5/08

    CPC分类号： A01N1/0221 ,  A01N1/02 ,  C12N5/0606

    摘要： A cryopreservation medium and a cryopreservation method which make it possible to cryopreserve ES cells from primates simply with high viability are provided. A cryopreservation medium containing a cryoprotectant at a Concentration of from 12% (W/V) to 50% (W/V) and a cryopreservation method for primate embryonic stem cells, which comprises a step of suspending primate embryonic stem cells in the cryopreservation medium, and a refrigeration step of freezing the suspension of the primate embryonic stem cells in the cryopreservation medium by cooling it to −80° C. or below at a rate of from 0.5° C. to 10° C. per minute, and a preservation step of storing the frozen suspension of primate embryonic stem cells in the cryopreservation medium enable simple cryopreservation of primate embryonic stem cells with high viability.

    摘要翻译： 提供了一种冷冻保存培养基和一种冷冻保存方法，其可以简单地以高活力从灵长类动物中冷冻保存ES细胞。 含有浓度为12％（W ​​/ V）至50％（W / V）的冷冻保护剂的冷冻保存培养基和用于灵长类动物胚胎干细胞的冷冻保存方法，其包括将灵长类动物胚胎干细胞悬浮于冷冻保存培养基 以及冷冻步骤，其通过以0.5℃至10℃/分钟的速率将冷冻至-80℃或更低的冷冻保存在冷冻保存介质中的灵长类动物胚胎干细胞的悬浮液进行冷冻和保存 将冷冻的灵长类动物胚胎干细胞的悬浮液储存在冷冻保存培养基中的步骤能够实现具有高活力的灵长类动物胚胎干细胞的简单的低温保存。

    METHOD FOR REMOVING DESIRED CHROMOSOME AND TAILOR-MADE MEDICAL TREATMENT UTILIZING THE SAME
    5.
    发明申请
    METHOD FOR REMOVING DESIRED CHROMOSOME AND TAILOR-MADE MEDICAL TREATMENT UTILIZING THE SAME 审中-公开
    移除所需染色体和使用其的定制化妆品医疗治疗方法

    公开(公告)号：US20090264312A1

    公开(公告)日：2009-10-22

    申请号：US11813930

    申请日：2006-01-12

    申请人： Takashi Tada ,  Norio Nakatsuji ,  Hiroyuki Matsumura ,  Masako Tada

    发明人： Takashi Tada ,  Norio Nakatsuji ,  Hiroyuki Matsumura ,  Masako Tada

    IPC分类号： C40B40/02 ,  C12N15/74 ,  C12N15/00 ,  C12N5/10

    CPC分类号： A61K35/48 ,  C12N5/0081 ,  C12N5/12 ,  C12N15/907 ,  C12N2510/00 ,  C12N2800/30 ,  C12N2840/203

    摘要： It is intended to provide a regenerable cell in which a desired chromosome has been deleted, a method for producing the cell, and a gene cassette and a kit to be used for the method. More particularly, it is intended to obtain an individual corresponding pluripotent stem cell easily and simply. More specifically, it is intended to efficiently establish a cell, tissue or organ that can be a donor for treating a disease without causing immune rejection response, without newly obtaining and establishing a stem cell such as ES cell from the individual, and without using an ovum as a material. It was achieved by a gene cassette in which not two recombinase recognition sites in a cis orientation are inserted, but one recombinase recognition site or two recombinase recognition sites in an opposite orientation is/are inserted and a marker gene is connected, and by application of the gene cassette to a cell fusion technique.

    摘要翻译： 旨在提供其中缺失所需染色体的可再生细胞，生产细胞的方法，以及用于该方法的基因盒和试剂盒。 更具体地，旨在容易且简单地获得单个相应的多能干细胞。 更具体地，旨在有效地建立可以是用于治疗疾病而不引起免疫排斥反应的供体的细胞，组织或器官，而不从个体中新获得和建立诸如ES细胞的干细胞，并且不使用 卵子为材料。 通过插入不具有顺式取向的两个重组酶识别位点的基因盒来实现，但是插入一个相反方向的一个重组酶识别位点或两个重组酶识别位点，并连接标记基因，并通过应用 基因盒到细胞融合技术。

    Tailor-made pluripotent stem cell and use of the same
    8.
    发明申请
    Tailor-made pluripotent stem cell and use of the same 审中-公开
    定制多能干细胞及其使用

    公开(公告)号：US20080003560A1

    公开(公告)日：2008-01-03

    申请号：US11818935

    申请日：2007-06-14

    申请人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

    发明人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

    IPC分类号： C12N15/07 ,  C12N5/06

    CPC分类号： A61L27/3895 ,  A61K2035/122 ,  A61L27/3604 ,  A61L27/3834 ,  C12N5/0606 ,  C12N5/16 ,  C12N2510/00 ,  C12N2510/02

    摘要： An object of the present invention is to efficiently establish cells, tissues, and organs capable of serving as donors for treating diseases, without eliciting immune rejection reactions, without starting with an egg cell. This object was achieved by providing a pluripotent stem cell having a desired genome. The cell was produced by treating with a reprogramming agent, producing a fusion cell of an MHC deficient stem cell with a somatic cell, or after producing a fusion cell of a stem cell with a somatic cell, removing a gene derived from the stem cell by performing genetic manipulation with a retrovirus.

    摘要翻译： 本发明的一个目的是有效地建立能够作为用于治疗疾病的供体的细胞，组织和器官，而不引发免疫排斥反应，而不是从卵细胞开始。 该目的通过提供具有所需基因组的多能干细胞来实现。 通过用编程剂处理细胞，产生具有体细胞的MHC缺陷型干细胞的融合细胞，或在产生具有体细胞的干细胞的融合细胞后，通过以下步骤除去源自干细胞的基因： 用逆转录病毒进行遗传操作。

    Marker for undifferentiated state of cell and composition and method for separation and preparation of stem cells
    9.
    发明申请
    Marker for undifferentiated state of cell and composition and method for separation and preparation of stem cells 审中-公开
    细胞未分化状态的标记物及其分离和制备干细胞的方法

    公开(公告)号：US20060288431A1

    公开(公告)日：2006-12-21

    申请号：US10545368

    申请日：2004-02-12

    申请人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

    发明人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

    IPC分类号： A01K67/027 ,  C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C07K14/705 ,  C07K16/28

    CPC分类号： C07K14/47 ,  C12N5/0607 ,  C12N2510/00

    摘要： A gene is provided, which can be used as a marker for determining whether a certain cell, particularly an undifferentiated cell including a tissue stem cell, has pluripotency or an undifferentiated state. The gene is called Stm and includes a Stm1 gene, which is expressed specifically in a cell under an undifferentiated state if the cell has pluripotency. A kit for determining a differentiated state of a cell is also provided. The kit comprises (a) an agent capable of reacting specifically with a Stm gene or a Stm gene product; and (b) means for determining whether or not the Stm gene is expressed in the cell.

    摘要翻译： 提供了可用作确定特定细胞，特别是包括组织干细胞的未分化细胞是否具有多能性或未分化状态的标记的基因。 该基因称为Stm，包括Stm1基因，如果细胞具有多能性，其在未分化状态的细胞中特异性表达。 还提供了用于确定细胞分化状态的试剂盒。 试剂盒包含（a）能够与Stm基因或Stm基因产物特异性反应的试剂; 和（b）用于确定Stm基因是否在细胞中表达的手段。