公开(公告)号：US06596521B1

公开(公告)日：2003-07-22

申请号：US09529570

申请日：2000-04-13

申请人： Ho Nam Chang ,  Yong Keun Chang ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Pyung Cheon Lee ,  Ik Keun Yoo ,  Seong Jin Lim

发明人： Ho Nam Chang ,  Yong Keun Chang ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Pyung Cheon Lee ,  Ik Keun Yoo ,  Seong Jin Lim

IPC分类号： C12P740

CPC分类号： C12P7/46 ,  C12M23/58 ,  C12M29/18 ,  C12M41/26 ,  C12P7/40 ,  C12P7/42 ,  C12P7/48

摘要： The present invention provides a method for manufacturing organic acid by high-efficiency fermentation, which comprises the steps of continuous culture of organic acid bacteria and collection of organic acid produced from the culture employing a cell-recycle multiple-stage continuous fermentor with serially connected fermentors, each of which comprises a fermentor containing a ferment container, temperature controller, stirrer, and pH controller; pumps for efflux-circulation of media from the fermentor; and, cell separator for separation and circulation of media from the pumps. According to the present method, the high-concentration lactic acid of 90 g/L can be produced with the high productivity of 50 g/L/h, which can reduce the facility cost and production cost in the bulk manufacturing process. In addition, the present invention can be effectively applied to the production of other organic acids such as acetic acid, formic acid, citric acid, malic acid, maleic acid, fumaric acid, and succinic acid, which show the end-product inhibition.

摘要翻译： 本发明提供了一种通过高效发酵制造有机酸的方法，其包括以下步骤：连续培养有机酸细菌和收集由使用连续发酵罐的细胞循环多级连续发酵罐的培养物产生的有机酸 ，其中每个包含含有发酵容器，温度控制器，搅拌器和pH控制器的发酵罐; 用于来自发酵罐的介质的外排循环的泵; 以及用于从泵分离和循环介质的细胞分离器。 根据本方法，可以以50g / L / h的高生产率生产90g / L的高浓度乳酸，这可以降低批量制造过程中的设备成本和生产成本。 此外，本发明可以有效地应用于生产其它有机酸如乙酸，甲酸，柠檬酸，苹果酸，马来酸，富马酸和琥珀酸，这些有机酸显示出最终产物的抑制作用。

公开(公告)号：US06406628B1

公开(公告)日：2002-06-18

申请号：US09398390

申请日：1999-09-17

申请人： Ho Nam Chang ,  Seong Jin Lim ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Dong Won Choi

发明人： Ho Nam Chang ,  Seong Jin Lim ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Dong Won Choi

IPC分类号： C02F300

CPC分类号： C02F3/1263 ,  C02F3/308 ,  Y02W10/15

摘要： The present invention provides to a method for removing nitrogen and phosphorous in wastewater simultaneously from wastewater by employing fermentation broth obtained by anaerobic fermentation of organic solid wastes. The method for removing nitrogen and phosphorous in wastewater of the invention comprises the steps of subjecting organic waste material to anaerobic fermentation at 30 to 40° C. for 2 to 4 days by using anaerobic sludge to obtain fermentation broth; and, adding the fermentation broth to a sequencing batch reactor with alternating anaerobic-aerobic-anoxic cycles under anaerobic and anoxic conditions in a separate manner. In accordance with the present invention, nitrogen in the influent wastewater can be removed more than 95% in total and phosphorous concentration can be kept below 1 ppm regardless of the concentration of organic materials in the influent wastewater. Further, the present method requires no extra pretreatment steps or chemicals which are essential to remove residual ammonia nitrogen in the prior art systems, and lowers the overall cost for wastewater treatment due to the usage of organic waste materials.

摘要翻译： 本发明提供一种通过使用通过有机固体废物的厌氧发酵获得的发酵液，从废水中同时除去废水中的氮和磷的方法。 本发明废水中除氮和磷的方法包括以下步骤：通过使用厌氧污泥将有机废物在30〜40℃进行厌氧发酵2〜4天，得到发酵液; 并将发酵液添加到在厌氧和缺氧条件下以分开的方式交替的厌氧 - 需氧 - 缺氧循环的测序间歇反应器。 根据本发明，流入废水中的氮总量可以去除95％以上，磷浓度可以保持在1ppm以下，而与进水废水中有机物质的浓度无关。 此外，本方法不需要额外的预处理步骤或在现有技术系统中除去残余氨氮所必需的化学品，并且由于使用有机废物材料而降低了废水处理的总体成本。