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公开(公告)号：US08114591B2
公开(公告)日：2012-02-14
申请号：US11625844
申请日：2007-01-23
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： A method of observing reaction intermediaries during a chemical reaction and comprising detecting an electrical signal output from an ion sensitive field effect transistor exposed to said reaction, and monitoring the detected electrical signal to discriminate discrete fluctuations in the electrical signal, the discrete fluctuations indicating reaction intermediaries occurring during a chemical reaction.
摘要翻译： 一种在化学反应期间观察反应中间体的方法，包括检测暴露于所述反应的离子敏感场效应晶体管的电信号输出，并监测检测到的电信号以区分电信号中的离散波动，指示反应中间体的离散波动 在化学反应期间发生。
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公开(公告)号：US08685228B2
公开(公告)日：2014-04-01
申请号：US12711249
申请日：2010-02-23
IPC分类号： G01N27/327
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： A sensing apparatus comprising an ion sensitive field effect transistor arranged to generate an electrical output signal in response to localized fluctuations of ionic charge at or adjacent the surface of the transistor, and means for detecting the electrical output signal from the ion sensitive field effect transistor, the localized fluctuations of ionic charge indicating events occurring during a chemical reaction.
摘要翻译： 一种感测装置，包括离子敏感场效应晶体管，其布置成响应于晶体管的表面处或邻近表面处的离子电荷的局部波动而产生电输出信号，以及用于检测来自离子敏感场效应晶体管的电输出信号的装置， 离子电荷的局部波动指示化学反应期间发生的事件。
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公开(公告)号：US20100151479A1
公开(公告)日：2010-06-17
申请号：US12711249
申请日：2010-02-23
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： A sensing apparatus comprising an ion sensitive field effect transistor arranged to generate an electrical output signal in response to localised fluctuations of ionic charge at or adjacent the surface of the transistor, and means for detecting the electrical output signal from the ion sensitive field effect transistor, the localised fluctuations of ionic charge indicating events occurring during a chemical reaction.
摘要翻译： 一种感测装置，包括离子敏感场效应晶体管，其布置成响应于晶体管的表面处或邻近表面处的离子电荷的局部波动而产生电输出信号，以及用于检测来自离子敏感场效应晶体管的电输出信号的装置， 离子电荷的局部波动指示化学反应期间发生的事件。
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公开(公告)号：US07686929B2
公开(公告)日：2010-03-30
申请号：US10471197
申请日：2002-03-11
IPC分类号： G01N27/327 , G01N27/333 , G01N27/414
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： A sensing apparatus comprising an ion sensitive field effect transistor arranged to generate an electrical output signal in response to localized fluctuations of ionic charge at or adjacent the surface of the transistor, and means for detecting the electrical output signal from the ion sensitive field effect transistor, the localized fluctuations of ionic charge indicating events occurring during a chemical reaction.
摘要翻译： 一种感测装置，包括离子敏感场效应晶体管，其布置成响应于晶体管的表面处或邻近表面处的离子电荷的局部波动而产生电输出信号，以及用于检测来自离子敏感场效应晶体管的电输出信号的装置， 离子电荷的局部波动指示化学反应期间发生的事件。
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公开(公告)号：US20120175252A1
公开(公告)日：2012-07-12
申请号：US13352063
申请日：2012-01-17
IPC分类号： G01N27/414 , G01N33/50
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： A method of observing reaction intermediaries during a chemical reaction and comprising detecting an electrical signal output from an ion sensitive field effect transistor exposed to said reaction, and monitoring the detected electrical signal to discriminate discrete fluctuations in the electrical signal, the discrete fluctuations indicating reaction intermediaries occurring during a chemical reaction.
摘要翻译： 一种在化学反应期间观察反应中间体的方法，包括检测暴露于所述反应的离子敏感场效应晶体管的电信号输出，并监测检测到的电信号以区分电信号中的离散波动，指示反应中间体的离散波动 在化学反应期间发生。
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公开(公告)号：US07888015B2
公开(公告)日：2011-02-15
申请号：US11681212
申请日：2007-03-02
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： Use of a pH sensor comprising an ion-sensitive field effect transistor (ISFET) to perform real time detection/quantification of nucleic acid amplification, e.g. polymerase chain reaction (PCR) nucleic acid amplification, based on detection of protons released during the primer extension phase.
摘要翻译： 使用包含离子敏感场效应晶体管（ISFET）的pH传感器来进行核酸扩增的实时检测/定量，例如， 基于检测在引物延伸期释放的质子的聚合酶链反应（PCR）核酸扩增。
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公开(公告)号：US20100255595A1
公开(公告)日：2010-10-07
申请号：US11625844
申请日：2007-01-23
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： A method of observing reaction intermediaries during a chemical reaction and comprising detecting an electrical signal output from an ion sensitive field effect transistor exposed to said reaction, and monitoring the detected electrical signal to discriminate discrete fluctuations in the electrical signal, the discrete fluctuations indicating reaction intermediaries occurring during a chemical reaction.
摘要翻译： 一种在化学反应期间观察反应中间体的方法，包括检测暴露于所述反应的离子敏感场效应晶体管的电信号输出，并监测检测到的电信号以区分电信号中的离散波动，指示反应中间体的离散波动 在化学反应期间发生。
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公开(公告)号：US08698211B2
公开(公告)日：2014-04-15
申请号：US13352063
申请日：2012-01-17
IPC分类号： G01N27/403 , C12M1/00
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： A method of observing reaction intermediaries during a chemical reaction and comprising detecting an electrical signal output from an ion sensitive field effect transistor exposed to said reaction, and monitoring the detected electrical signal to discriminate discrete fluctuations in the electrical signal, the discrete fluctuations indicating reaction intermediaries occurring during a chemical reaction.
摘要翻译： 一种在化学反应期间观察反应中间体的方法，包括检测暴露于所述反应的离子敏感场效应晶体管的电信号输出，并监测检测到的电信号以区分电信号中的离散波动，指示反应中间体的离散波动 在化学反应期间发生。
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公开(公告)号：US20080032295A1
公开(公告)日：2008-02-07
申请号：US11681212
申请日：2007-03-02
CPC分类号： C12Q1/6825 , C12Q2565/607 , G01N27/4145
摘要： Use of a pH sensor comprising an ion-sensitive field effect transistor (ISFET) to perform real time detection/quantification of nucleic acid amplification, e.g. polymerase chain reaction (PCR) nucleic acid amplification, based on detection of protons released during the primer extension phase.
摘要翻译： 使用包含离子敏感场效应晶体管（ISFET）的pH传感器来执行核酸扩增的实时检测/定量，例如， 基于检测在引物延伸期释放的质子的聚合酶链反应（PCR）核酸扩增。
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