Porous polymer particle, anion exchanger, producing method thereof, column for ion chromatography, and method for measuring anions
    1.
    发明授权
    Porous polymer particle, anion exchanger, producing method thereof, column for ion chromatography, and method for measuring anions 有权
    多孔聚合物颗粒,阴离子交换剂,其制备方法,离子色谱柱和阴离子测定方法

    公开(公告)号:US06881761B2

    公开(公告)日:2005-04-19

    申请号:US09799806

    申请日:2001-03-07

    摘要: Disclosed are (1) a porous polymer particle comprising a polymer substrate having ester bonds connected to a group containing a quaternary ammonium structure through a spacer molecule, (2) an alkali-resistant, high-strength porous polymer particle having ester bonds connected to a group containing a quaternary ammonium structure through a spacer molecule, a part of the ester bonds being saponified and converted to a hydroxyl group(s), (3) a porous polymer particle comprising a polymer substrate having ester bonds connected to a group containing a quaternary ammonium structure through a spacer molecule, the porous polymer particles being treated with an alkaline solution to generate a hydroxyl and/or a carboxyl group on a surface of the substrate, (4) an anion exchanger comprising any one of the particles (1) to (3) above, (5) a packing material for anion chromatography comprising the anion exchanger (4) above, (6) a column for anion chromatography using the anion exchanger (4) above, and (7) a method for measuring anions using the anion exchanger (4) above.

    摘要翻译: 公开了(1)一种多孔聚合物颗粒,其包含具有通过间隔分子连接到含有季铵结构的基团的酯键的聚合物基材,(2)具有连接到酯基的酯键的耐碱性高强度多孔聚合物颗粒 通过间隔分子含有季铵结构的基团,一部分酯键被皂化并转化成羟基,(3)多孔聚合物颗粒,其包含具有连接到含有四价基团的酯键的聚合物底物 通过间隔分子的铵结构,所述多孔聚合物颗粒用碱性溶液处理以在所述基材的表面上产生羟基和/或羧基,(4)阴离子交换剂,其包含颗粒(1)至 (3),(5)阴离子色谱用填充材料,其包含上述阴离子交换体(4),(6)使用上述阴离子交换体(4)的阴离子色谱柱, nd(7)使用上述阴离子交换器(4)测量阴离子的方法。

    Lipase, microorganisms producing the lipase, method of producing the
lipase and use of the lipase
    2.
    发明授权
    Lipase, microorganisms producing the lipase, method of producing the lipase and use of the lipase 失效
    脂肪酶,产生脂肪酶的微生物,生产脂肪酶的方法和脂肪酶的使用

    公开(公告)号:US5827718A

    公开(公告)日:1998-10-27

    申请号:US605015

    申请日:1996-03-14

    摘要: Bacteria belonging to the genus Pseudomonas, alkaline lipase produced by the bacteria and having the following properties, a method of producing the lipase, and detergent compositions containing the lipase: (1) Operating pH and optimum pH an operating pH is in the range of from 3.5 to 12 and an optimum pH is in the range of from 10 to 11 using a triolein emulsion as a substrate; (2) Operating temperature and optimum temperature an operating temperature is in the range of from 30.degree. C. to 80.degree. C. and an optimum temperature is in the range of from 55.degree. C. to 65.degree. C. using the triolein emulsion as a substrate;(3) Molecular weight a molecular weight measured by SDS-polyacrylamide gel electrophoresis is 31,000.+-.2,000; and (4) Isoelectric point an Isoelectric point measured by isoelectric point polyacrylamide gel electrophoresis is 5.2.+-.0.5. The lipase has high stability against detergent components such as surfactants, protease, etc. and can be blended together with protease with detergents. Further, the lipase suffers less inhibition of its activity, so that it can enhance the washing power of detergents containing it.

    摘要翻译: PCT No.PCT / JP94 / 01416 Sec。 371日期:1996年3月14日 102(e)1996年3月14日PCT PCT 1994年8月26日PCT公布。 公开号WO95 / 06720 1995年3月9日属于假单胞菌属的细菌,由细菌产生并具有以下性质的碱性脂肪酶,生产脂肪酶的方法和含有脂肪酶的洗涤剂组合物:(1)操作pH和最适pH,操作pH为 在3.5至12的范围内,并且使用三油酸酯乳液作为底物,最佳pH在10至11的范围内; (2)工作温度和最佳温度,工作温度在30℃至80℃的范围内,最佳温度为55℃至65℃,使用三油酸酯乳液作为 底物; (3)通过SDS-聚丙烯酰胺凝胶电泳测定的分子量分子量为31,000 +/- 2,000; 和(4)等电点通过等电点聚丙烯酰胺凝胶电泳测量的等电点为5.2 +/- 0.5。 脂肪酶对洗涤剂组分如表面活性剂,蛋白酶等具有高稳定性,并且可与蛋白酶一起与洗涤剂共混。 此外,脂肪酶对其活性的抑制较少,从而可以提高含有它的洗涤剂的洗涤能力。

    Thermostable lipase isolated from Pseudomonas solanacearum
    3.
    发明授权
    Thermostable lipase isolated from Pseudomonas solanacearum 失效
    从番茄假单胞菌分离的热稳定脂肪酶

    公开(公告)号:US5846801A

    公开(公告)日:1998-12-08

    申请号:US860982

    申请日:1997-07-15

    IPC分类号: C12N1/20 C12N9/20 C12R1/38

    CPC分类号: C12N9/20

    摘要: A thermostable lipase has been isolated from Pseudomonas solanacearum SD709 (FERM BP-5358) which has a mass of 32 kD by SDS-PAGE, enzymatic activity in a pH range of about 4-12, a pH optimum of 6.5-9.5 and a temperature optimum of 80.degree.-90.degree. C.

    摘要翻译: PCT No.PCT / JP96 / 00454 Sec。 371日期1997年7月15日 102(e)日期1997年7月15日PCT提交1996年2月27日PCT公布。 公开号WO96 / 27659 日期1996年9月12日热稳定脂肪酶已经从Solanacearum SD709(FERM BP-5358)中分离,其质量为32kD,通过SDS-PAGE,酶活性在约4-12的pH范围内,pH最佳值为6.5 -9.5,最适温度为80-90℃。

    Modified polymer particle, packing material and column for chromatography
    4.
    发明申请
    Modified polymer particle, packing material and column for chromatography 审中-公开
    改性聚合物颗粒,填料和色谱柱

    公开(公告)号:US20060052531A1

    公开(公告)日:2006-03-09

    申请号:US10527096

    申请日:2003-09-11

    IPC分类号: C08G63/91

    CPC分类号: C08G63/91

    摘要: An object of the present invention is to prevent an isolation of carboxyl group caused by hydrolysis of ester bond, which occurs when a polymer particle containing an ester bond is used in the presence of a strongly acidic or strongly alkaline solution to thereby improve the acid Ealkali durability of the polymer particle. When a polymer particle containing an ester bond is treated with an acid or alkali hydrous solution and thereby an ester bond which is located and exposed on the surface of the particle and is readily hydrolyzed is previously hydrolyzed to isolate a carboxyl group and then the free carboxyl group is capped by amidation, to thereby dissolve the above problem.

    摘要翻译: 本发明的目的是防止在强酸性或强碱性溶液的存在下使用含有酯键的聚合物粒子时酯键的水解引起的羧基的分离,从而改善酸的碱性 聚合物颗粒的耐久性。 当用酸或碱水溶液处理含有酯键的聚合物颗粒时,预先水解位于并暴露在颗粒表面并容易水解的酯键以分离羧基,然后将游离羧基 组被酰胺化所限制,从而解决了上述问题。