公开(公告)号：US09447388B2

公开(公告)日：2016-09-20

申请号：US14232174

申请日：2012-07-12

申请人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

发明人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

IPC分类号： C12N9/12 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34

CPC分类号： C12N9/1252 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34 ,  C12Y207/07007 ,  Y02P20/52

摘要： Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737,wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).

公开(公告)号：US20140322793A1

公开(公告)日：2014-10-30

申请号：US14232174

申请日：2012-07-12

申请人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

发明人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

IPC分类号： C12N9/12

CPC分类号： C12N9/1252 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34 ,  C12Y207/07007 ,  Y02P20/52

摘要： Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737,wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).

摘要翻译： 提供各种新型DNA聚合酶。 本发明提供一种DNA聚合酶，其包含在SEQ ID NO：8的氨基酸序列之间插入9个氨基酸“-A737-A738-A739-A740-A741-A742-A743-A744-A745-”的氨基酸序列 位置736处的氨基酸残基和737位的氨基酸残基，其中：A737是具有非极性脂肪族侧链的氨基酸残基; A738是具有非极性脂肪族侧链的氨基酸残基; A739是具有带正电侧链的氨基酸残基; A740是具有带正电荷侧链的氨基酸残基; A741是具有非极性脂肪族侧链的氨基酸残基; A742是具有非极性脂肪族侧链的氨基酸残基; A743是任何给定的氨基酸残基; A744是具有带正电侧链的氨基酸残基; A745是具有非极性脂肪族侧链的氨基酸残基）。

公开(公告)号：US20080206811A1

公开(公告)日：2008-08-28

申请号：US11597318

申请日：2005-08-19

申请人： Toshihiro Shodai ,  Hiroshi Kobori ,  Takehiro Sagara ,  Hiroshi Endo ,  Hikaru Takakura ,  Jun Tomono ,  Hiroaki Sagawa ,  Hiroyuki Mukai ,  Ikunoshin Kato

发明人： Toshihiro Shodai ,  Hiroshi Kobori ,  Takehiro Sagara ,  Hiroshi Endo ,  Hikaru Takakura ,  Jun Tomono ,  Hiroaki Sagawa ,  Hiroyuki Mukai ,  Ikunoshin Kato

IPC分类号： C12P21/04 ,  C12N15/00

CPC分类号： C12N15/70 ,  C12N9/1276 ,  C12N9/22 ,  C12P21/02

摘要： A process for producing a target protein at low temperature, comprising inducing expression of not only a vector having introduced therein a gene coding for the target protein but also a vector having a chaperone gene introduced therein.

摘要翻译： 一种在低温下生产靶蛋白的方法，包括不仅引入其中引入了编码靶蛋白的基因的载体，还诱导其中引入伴侣伴侣基因的载体。