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公开(公告)号:US5686253A
公开(公告)日:1997-11-11
申请号:US450744
申请日:1995-05-25
IPC分类号: C12N5/10 , C12N9/96 , C12N15/02 , C12N15/09 , C12P21/08 , C12Q1/26 , C12Q1/28 , C12Q1/32 , C12Q1/68 , G01N33/535 , G01N33/542 , G01N33/573 , G01N33/577
CPC分类号: G01N33/542 , C12N9/96 , G01N33/535 , Y10S435/963 , Y10S435/975 , Y10S436/822
摘要: A method is disclosed for stabilizing a conjugate of an enzyme and a member of a specific binding pair (enzyme conjugate). The method comprises the step of combining the enzyme conjugate with an effective amount of an antibody for the enzyme where the antibody does not substantially inhibit the activity of the enzyme. The invention has application to assays for the determination of an analyte wherein enzyme conjugates are employed. The improvement comprises employing as a reagent in the assay an immune complex of an enzyme conjugate and an antibody for the enzyme where the antibody does not substantially inhibit the activity of the enzyme. Compositions comprising such an immune complex and kits comprising such an immune complex in packaged combination with other assay reagents are also disclosed.
摘要翻译: 公开了用于稳定酶和特异性结合对(酶结合物)成员的缀合物的方法。 该方法包括将酶结合物与酶的有效量的抗体组合的步骤,其中抗体基本上不抑制酶的活性。 本发明应用于测定其中使用酶缀合物的分析物的测定。 该改进包括在测定中使用酶缀合物的免疫复合物和酶的抗体,其中抗体基本上不抑制酶的活性。 还公开了包含这种免疫复合物的组合物和包含与其它测定试剂的包装组合中的这种免疫复合物的试剂盒。