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公开(公告)号:US20050026239A1
公开(公告)日:2005-02-03
申请号:US10826922
申请日:2004-04-19
申请人: Carlos Castro , Richard Ridge , Thomas Novitsky
发明人: Carlos Castro , Richard Ridge , Thomas Novitsky
IPC分类号: C12M1/34 , C12Q1/04 , C12Q1/22 , G01N20060101 , G01N31/00 , G01N33/53 , G01N33/554 , G01N33/569 , G01N33/579
CPC分类号: G01N33/579 , G01N33/56911
摘要: The present invention relates to a simple, rapid, and cost-effective test kit for specifically detecting bacterial endotoxin in aqueous solutions, such as water or dialysate solutions, using a Limulus Amebocyte Lysate (LAL)-based gel clot assay. Advantageously, the test kit can vary in its level of sensitivity for detecting endotoxin.
摘要翻译: 本发明涉及一种简单,快速且具有成本效益的测试试剂盒,用于使用基于鲎A蛋白裂解物(LAL)的凝胶凝块测定法特异性检测水溶液(如水或透析液)中的细菌内毒素。 有利地,测试试剂盒可以在其检测内毒素的灵敏度水平方面变化。
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公开(公告)号:US20050106746A1
公开(公告)日:2005-05-19
申请号:US11009832
申请日:2004-12-10
申请人: Alan Shinn , Chiko Fan , Elias Elias , Thomas Novitsky , Michael Dawson , Keith Richardson
发明人: Alan Shinn , Chiko Fan , Elias Elias , Thomas Novitsky , Michael Dawson , Keith Richardson
CPC分类号: G01N21/51 , G01N21/253 , G01N21/272 , G01N21/532 , Y10T436/11
摘要: An analytical device that can be used to conveniently and accurately assay plural vessels. In one exemplary embodiment, a pair of LED sources provides illumination through a pair of radial waveguides to plural vessels arranged in a pair of substantially concentric and circular rows about the LED sources. A light pipe receives light transmitted through a vessel from each radial waveguide and reflects the received light downward to a single printed circuit board that contains a photodiode for each light pipe, as well as processing circuitry. The first LED source/radial waveguide optical is used to confirm the presence of a vessel, and the second is used to perform, e.g., turbidometric and/or colorimetric assays upon an analyte within the vessel. The vessel is incubated in a vessel support that includes a heat conducting base and a heat insulating cover. Heat is supplied by a DC heater.
摘要翻译: 可用于方便,准确地测定多个血管的分析装置。 在一个示例性实施例中,一对LED源通过一对径向波导提供照射到围绕LED源布置在一对基本同心和圆形行中的多个容器。 光管接收从每个径向波导传输通过容器的光,并将接收的光向下反射到包含用于每个光管的光电二极管的单个印刷电路板以及处理电路。 第一个LED源/径向波导光学器件用于确认容器的存在,第二个用于对容器内的分析物进行例如浊度和/或比色测定。 将容器在包括导热基底和隔热盖的容器支架中孵育。 热量由直流加热器供电。
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公开(公告)号:US20050048655A1
公开(公告)日:2005-03-03
申请号:US10867162
申请日:2004-06-14
申请人: Thomas Novitsky , Richard Ridge , Carlos Castro
发明人: Thomas Novitsky , Richard Ridge , Carlos Castro
IPC分类号: C12Q1/04 , C12Q1/34 , G01N31/00 , G01N33/554 , G01N33/569
CPC分类号: G01N33/56911 , G01N33/579 , Y10T436/10
摘要: Kits and method for detecting bacterial endotoxin in an aqueous solution are provided. In certain examples, the kit includes at least a first container comprising solid, endotoxin-specific, horseshoe crab amebocyte lysate, whereby the sensitivity of the amebocyte lysate is pre-certified. In certain examples, the kit also contains at least a second container comprising a defined quantity of endotoxin configured as a positive product control, wherein the defined quantity of the endotoxin is pre-certified to react positively with the amebocyte lysate in the first container.
摘要翻译: 提供了在水溶液中检测细菌内毒素的试剂盒和方法。 在某些实施例中,该试剂盒包括至少第一容器,其包含固体内毒素特异性马蹄形细胞裂解物,由此预先验证了血小板裂解物的敏感性。 在某些实施例中,该试剂盒还含有至少一个第二容器,该第二容器包含配置为阳性产物对照物的限定量的内毒素,其中所述限定量的内毒素被预认证以与第一容器中的微血管细胞溶解产物正相反应。
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公开(公告)号:US20050069972A1
公开(公告)日:2005-03-31
申请号:US10897979
申请日:2004-07-23
申请人: Carlos Castro , Richard Ridge , Thomas Novitsky
发明人: Carlos Castro , Richard Ridge , Thomas Novitsky
IPC分类号: C12Q1/04 , C12Q1/34 , G01N31/00 , G01N33/554 , G01N33/569
CPC分类号: G01N33/56911 , G01N33/579 , Y10T436/10
摘要: Kits and method for detecting bacterial endotoxin in an aqueous solution are provided. In certain examples, the kit includes at least a first container comprising solid, endotoxin-specific, horseshoe crab amebocyte lysate and at least one buffer, whereby the sensitivity of the amebocyte lysate is pre-certified. In certain examples, the kit also contains at least a second container comprising a defined quantity of endotoxin configured as a positive product control, wherein the defined quantity of the endotoxin is pre-certified to react positively with the amebocyte lysate in the first container.
摘要翻译: 提供了在水溶液中检测细菌内毒素的试剂盒和方法。 在某些实施例中,试剂盒包括至少第一容器,其包含固体,内毒素特异性,马蹄蟹变形细胞溶解产物和至少一种缓冲液,由此对所述微生物细胞裂解液的敏感性进行预认证。 在某些实施例中,该试剂盒还含有至少一个第二容器,该第二容器包含配置为阳性产物对照物的限定量的内毒素,其中所述限定量的内毒素被预认证以与第一容器中的微血管细胞溶解产物正相反应。
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