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公开(公告)号：US20240254419A1
公开(公告)日：2024-08-01
申请号：US18400922
申请日：2023-12-29
摘要： An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.
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公开(公告)号：US20240182833A1
公开(公告)日：2024-06-06
申请号：US18512262
申请日：2023-11-17
摘要： The current invention relates to a method for obtaining one or more target biomolecules from cells cultured in a fixed bed bioreactor, said method comprises: the addition of a lysis solution to the bioreactor, thereby lysing the cells in the bioreactor, and prior to, simultaneous with, and/or after lysing said cells, applying one or more mechanical actions to said bioreactor, after which the one or more target biomolecules are recovered from said bioreactor. The invention further relates to a system for obtaining one or more target biomolecules from cells cultured in a fixed bed bioreactor.
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公开(公告)号：US20210155892A1
公开(公告)日：2021-05-27
申请号：US17263402
申请日：2019-06-27
发明人： Bastien Mairesse , Laetitia De Viron , Alex Chatel
摘要： The current disclosure concerns a system for producing biomolecules comprising a docking station (30), said docking station encompasses: —a bioreactor (1); —a concentrator (2) fluidly linked to the bioreactor; —an intermediate vessel (4), positioned between said bioreactor (1) and concentrator (2), wherein said intermediate vessel (4) and concentrator (2) are connected by a retentate conduit (303), allowing recirculating of liquid from an output of the concentrator to an input of said intermediate vessel and —a controller, integrated in said docking station (30), which is able to control the biomolecule process.
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公开(公告)号：US20210047599A1
公开(公告)日：2021-02-18
申请号：US16933398
申请日：2020-07-20
摘要： The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.
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公开(公告)号：US12036549B2
公开(公告)日：2024-07-16
申请号：US16347961
申请日：2017-11-08
发明人： José Castillo , Bastien Mairesse
CPC分类号： B01L3/502715 , B01L1/04 , C12M23/52 , C12M37/00 , C12N7/00 , B01L2300/06
摘要： The invention provides a method and a system for the production of viruses or virus derived products. The system comprises a containment enclosure provided with a single entry means and a single exit means which are movable between a closed position and an open position. The containment enclosure comprises at least one production unit comprising at least one bioreactor for culturing the virus, said bioreactor is provided with at least one inlet and at least one outlet; at least one purification unit fluidly connected to the production unit and comprising at least one purification means having at least one inlet and at least one outlet. The system further comprises at least one inactivation and/or at least one gas decontamination unit. The containment enclosure comprises at least one process control device connected to the exit means. The movement of the exit means from the closed position to the open position is controllable by said process control device.
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公开(公告)号：US20240209310A1
公开(公告)日：2024-06-27
申请号：US18288459
申请日：2022-04-29
摘要： A system for producing and purifying target biologics is disclosed. A cell culture unit is provided for generating target biologics in a volume of fluid. A purification unit is also provided for purifying the target biologics in the volume of fluid portion directly from the cell culture unit without a clarifying step, such as by using one or more separators, such as expanded beds or chromatography columns arranged in parallel, and potentially operating in series to achieve continuous operation. The one or more separators may include magnetic beads adapted for binding to the target biologics. The purification unit may receive at least a portion of the volume of fluid directly from the cell culture unit, and a collection unit may be provided for collecting the purified target biologics from the purification unit, and also may be adapted for returning the volume of fluid without the target biologics to the cell culture unit. The system may comprise a first vessel adapted to concentrate a volume of fluid including the target biologics, and a second vessel adapted to further concentrating the volume of fluid, the first or second vessels including magnetic beads adapted for binding with the target biologics. Related methods are also disclosed.
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公开(公告)号：US11946032B2
公开(公告)日：2024-04-02
申请号：US16981333
申请日：2019-03-18
发明人： José Castillo , Bastien Mairesse , Alex Chatel , Sebastien Jean-Pierre Michel Rodriguez , Alexandre VAnhaver
摘要： A sampler for use with a bioreactor for growing a cell culture is disclosed. In one embodiment, the bioreactor includes a structured fixed bed including a removable sample portion for recovering a sample of cells from the cell culture. Related apparatuses and methods are also disclosed.
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公开(公告)号：US20230272323A1
公开(公告)日：2023-08-31
申请号：US18028276
申请日：2021-09-27
摘要： A cell culturing container including a fixed bed is provided for forming a bioreactor (100). In one version, a container in the form of a tubular body is formed by first and second interlocking portions (115a, 115b). The container forms a compartment for receiving the fixed bed (122) for culturing cells, which container may be removably inserted into an inner compartment of the bioreactor so as form an outer chamber for circulating fluid to or from the fixed bed. The container may also include a removable base (130) for receiving an agitator (118) for agitating and thus circulating the fluid. Related methods are also disclosed.
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公开(公告)号：US20230146372A1
公开(公告)日：2023-05-11
申请号：US17981568
申请日：2022-11-07
摘要： An apparatus for sampling a cell culture includes a sampler for connecting to a bioreactor with a fixed bed including the cell culture. A releasably attached sample portion for positioning within or adjacent the fixed bed includes a substrate on which cells may be grown and then removed from the bioreactor to provide an indication of cell growth. A kit for sampling a cell culture bed of a bioreactor includes the sampler for sampling the cell culture bed with the releasably attached sample portion, along with a container adapted to detach the sample portion from the sampler. A method of obtaining a sample of a cell culture in a bed within a bioreactor includes positioning a sampler positioned at least partially in or adjacent to the bed to promote the growth of cells on a releasably attached portion of the sampler, withdrawing the sampler from the bioreactor, and detaching the portion of the sampler.
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10.发明申请公开(公告)号：US20210355428A1
公开(公告)日：2021-11-18
申请号：US17286269
申请日：2019-10-21
发明人： José Castillo , Bastien Mairesse , Sarah Beck
摘要： The current disclosure concerns a method for decontaminating a virus production system, wherein virus particles are produced in at least one bioreactor and purified from liquids comprising said virus particles thereby generating liquid waste, said method comprising the step of exposing said system or a part thereof to heated air and/or liquid at a temperature of at least 60° C. after viral production took place, thereby eradicating remaining virus particles in said system. In a second and third aspect the current disclosure concerns a virus production system wherein the system comprises a heating device suitable for heating air and/or liquid to a temperature of at least 60° C., and the use of such a system for the production of viruses and/or viral vaccines.
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