公开(公告)号：US5843634A

公开(公告)日：1998-12-01

申请号：US657392

申请日：1996-06-04

申请人： Elaine M Brate ,  Catherine A. Brennan ,  Dominique P. Bridon ,  Keeve D. Jaffe ,  Grant A. Krafft ,  Wlodzimierz Mandecki ,  Steven C. March ,  John C. Russell ,  Vincent T. Yue

发明人： Elaine M Brate ,  Catherine A. Brennan ,  Dominique P. Bridon ,  Keeve D. Jaffe ,  Grant A. Krafft ,  Wlodzimierz Mandecki ,  Steven C. March ,  John C. Russell ,  Vincent T. Yue

IPC分类号： G01N33/566 ,  C07K14/59 ,  C12N9/00 ,  C12N9/16 ,  C12N15/09 ,  C12N15/55 ,  C12Q1/00 ,  C12Q1/42 ,  C12R1/19 ,  C07K19/00 ,  C12Q1/44

CPC分类号： C07K14/59 ,  C12N9/16 ,  C12Q1/42 ,  C07K2319/40

摘要： This invention relates to genetically engineered enzymes, their ligand conjugates, their manufacture, and their use in qualitative or quantitative assays. A hybrid enzyme, such as an AP-epitope, has a foreign amino acid moiety (an epitope) inserted near the active site of the starting AP enzyme. The foreign amino acid moiety binds with an analyte, and, as a consequence of this binding, the enzymatic activity of the hybrid enzyme, AP-epitope, is modified. The changes in the enzymatic activity are dependent upon the presence, or the amount, of the analyte. In another embodiment, the hybrid enzyme consists of a cysteine introduced near the active site of an AP to give a hybrid enzyme. The cysteine on the hybrid enzyme serves as a point of conjugation of a ligand, such as theophylline, ferritin, thyroxine, or digoxigenin, to form the hybrid enzyme-ligand conjugate. The ligand binds with an antibody, an analyte or a binding molecule to an analyte and as a result of this binding, the enzymatic activity of the hybrid enzyme-ligand conjugate is modified or modulated.

摘要翻译： 本发明涉及基因工程改造的酶，其配体缀合物，其制备及其在定性或定量测定中的用途。 诸如AP-表位的杂交酶在起始AP酶的活性位点附近插入外源氨基酸部分（表位）。 外来氨基酸部分与分析物结合，并且作为该结合的结果，修饰了杂合酶AP-表位的酶活性。 酶活性的变化取决于分析物的存在或数量。 在另一个实施方案中，杂交酶由在AP的活性位点附近引入的半胱氨酸组成以产生杂交酶。 杂交酶上的半胱氨酸用作配体如茶碱，铁蛋白，甲状腺素或地高辛的缀合点，以形成杂交酶 - 配体缀合物。 配体与分析物结合抗体，分析物或结合分子，作为该结合的结果，修饰或调节杂交酶 - 配体结合物的酶活性。

公开(公告)号：US5270166A

公开(公告)日：1993-12-14

申请号：US859772

申请日：1992-03-30

申请人： Robert Parsons ,  Robert Kowal ,  Vincent T. Yue

发明人： Robert Parsons ,  Robert Kowal ,  Vincent T. Yue

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/556 ,  G01N33/58 ,  G01N33/546

CPC分类号： G01N33/53 ,  G01N33/54306 ,  G01N33/556 ,  G01N33/586 ,  Y10S435/807 ,  Y10S435/973 ,  Y10S436/816 ,  Y10S436/817 ,  Y10S436/822 ,  Y10S436/901

摘要： The present invention relates to immunoassay methods for detecting and measuring the amount of an analyte in a sample by means of generic anti-hapten antibodies. Also disclosed are multi-analyte immunoassay methods. Reagents, devices, and kits using the anti-hapten antibodies are also disclosed. The present invention also relates to dyed erythrocytes, preferably fixed, which are coated with antibodies. Also disclosed is the use of these dyed erythrocytes in agglutination assays to detect and measure the presence of an analyte in a sample. The analyte can be a hapten, an antigen, or an antibody. Also included are agglutination assays, compositions and kits using these dyed and coated erythrocytes.

摘要翻译： 本发明涉及通过通用抗半抗原抗体检测和测量样品中分析物的量的免疫测定方法。 还公开了多分析物免疫测定方法。 还公开了使用抗半抗原抗体的试剂，装置和试剂盒。 本发明还涉及用抗体包被的染色的红细胞，优选固定的。 还公开了这些染色的红细胞在凝集测定中用于检测和测量样品中分析物的存在的用途。 分析物可以是半抗原，抗原或抗体。 还包括使用这些染色和包被的红细胞的凝集测定法，组合物和试剂盒。