公开(公告)号：US20060246544A1

公开(公告)日：2006-11-02

申请号：US11396215

申请日：2006-03-30

申请人： Yun Kang ,  Walter Willett ,  Thomas Klimek ,  Basil Campbell ,  Paul Cino ,  Bradley Thomas ,  John Bermel ,  Chun-Chiang Chen

发明人： Yun Kang ,  Walter Willett ,  Thomas Klimek ,  Basil Campbell ,  Paul Cino ,  Bradley Thomas ,  John Bermel ,  Chun-Chiang Chen

IPC分类号： C12P21/06 ,  C12N9/24 ,  C12N5/06 ,  C07K14/53 ,  C07K14/51

CPC分类号： C07K14/535 ,  C07K1/20 ,  C07K14/505 ,  C07K14/51 ,  C07K14/53 ,  C12N9/1081 ,  C12P21/02 ,  C12Y204/99003

摘要： The present invention provides a manufacturing method for the production of peptides that are grown in insect cell lines. The peptides are grown in insect cell cultures that are infected with baculovirus particles in a culture supplemented with a lipid mixture. The peptides are then isolated from the insect cell culture using a method that employs a tangential flow filtration cascade. The isolated peptides are glycopeptides having an insect specific glycosylation pattern. The glycopeptides may then be conjugated to a modifying group via linkage through a glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase.

摘要翻译： 本发明提供了生产在昆虫细胞系中生长的肽的制备方法。 这些肽在补充有脂质混合物的培养物中被杆状病毒颗粒感染的昆虫细胞培养物中生长。 然后使用采用切向流过滤级联的方法从昆虫细胞培养物中分离肽。 分离的肽是具有昆虫特异性糖基化模式的糖肽。 然后可以通过插入在肽和修饰基团之间并且共价连接到肽和修饰基团上的糖基连接基团通过连接将糖肽与修饰基团缀合。 通过糖基转移酶的作用，由糖基化肽形成缀合物。

公开(公告)号：US20060287224A1

公开(公告)日：2006-12-21

申请号：US11440839

申请日：2006-05-25

申请人： Shawn DeFrees ,  Robert Bayer ,  David Zopf ,  Jichao Kang ,  Walter Willett

发明人： Shawn DeFrees ,  Robert Bayer ,  David Zopf ,  Jichao Kang ,  Walter Willett

IPC分类号： A61K38/16

CPC分类号： C07K14/505 ,  A61K38/1816 ,  A61K47/549 ,  A61K47/60

摘要： The present invention provides conjugates between erythropoietin and PEG moieties. The conjugates are linked via an intact glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto a glycosyl residue on the peptide. Also provided are methods for preparing the conjugates, methods for treating various disease conditions with the conjugates, and pharmaceutical formulations including the conjugates.

摘要翻译： 本发明提供红细胞生成素和PEG部分之间的缀合物。 缀合物通过插入和共价连接到肽和修饰基团之间的完整糖基连接基团连接。 通过糖基转移酶的作用，由糖基化肽形成缀合物。 糖基转移酶将修饰的糖部分连接到肽上的糖基残基上。 还提供了制备缀合物的方法，用缀合物治疗各种疾病状况的方法，以及包括缀合物的药物制剂。

公开(公告)号：US20070105755A1

公开(公告)日：2007-05-10

申请号：US11580669

申请日：2006-10-13

申请人： Shawn DeFrees ,  David Zopf ,  Susann Taudte ,  Walter Willett ,  Robert Bayer ,  Matthew Kalo

发明人： Shawn DeFrees ,  David Zopf ,  Susann Taudte ,  Walter Willett ,  Robert Bayer ,  Matthew Kalo

IPC分类号： A61K38/36 ,  C12P21/06

CPC分类号： C12P21/005 ,  A61K38/4846

摘要： The present invention provides conjugates between peptides and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.

摘要翻译： 本发明提供肽和PEG部分之间的缀合物。 缀合物经由完整的糖基连接基团连接，该基团介于并共价连接到肽和修饰基团之间。 通过糖基转移酶的作用，缀合物由糖基化和未糖基化的肽形成。 糖基转移酶将修饰的糖部分连接到肽上的氨基酸或糖基残基上。 还提供了包括缀合物的药物制剂。 缀合物的制备方法也在本发明的范围内。

公开(公告)号：US20070105192A1

公开(公告)日：2007-05-10

申请号：US10555123

申请日：2004-05-05

申请人： Joseph Villafranca ,  David Hakes ,  Karl Johnson ,  Walter Willett ,  Chester Meyers

发明人： Joseph Villafranca ,  David Hakes ,  Karl Johnson ,  Walter Willett ,  Chester Meyers

IPC分类号： C12P21/06 ,  C12P19/62 ,  C12P19/28 ,  C12P19/04 ,  C12N9/10

CPC分类号： C07H21/04 ,  C12N11/10 ,  C12P19/04 ,  C12P21/005

摘要： A method of immobilizing a molecular species that include a starch-binding domain is provided. There also is provided a material upon which the molecular species is immobilized, and a material that is capable of immobilizing the species The method includes binding the species to a solid support, e.g., membranes, chromatographic supports and the like. The immobilized species is optionally purified by the method of the invention. Alternatively, the immobilized species is use in another method, such as in a synthesis as a synthetic reagent, or to purify another species that has an affinity for the immobilized species. Exemplary immobilized molecular species include bioactive agents, and biomolecules.

摘要翻译： 提供了固定包含淀粉结合结构域的分子种的方法。 还提供了固定有分子种类的材料和能够固定物种的材料。该方法包括将物质结合到固体支持物，例如膜，色谱载体等。 任选地通过本发明的方法纯化固定化的物质。 或者，固定化物质用于另一种方法，例如在作为合成试剂的合成中，或用于纯化对固定化物质具有亲和性的另一物质。 示例性的固定化分子种类包括生物活性剂和生物分子。