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公开(公告)号：US20220403319A1
公开(公告)日：2022-12-22
申请号：US17765548
申请日：2021-02-04
发明人： Sean FARMER , Ken ALIBEK
摘要： The subject invention provides materials and methods for producing, isolating, extracting and purifying single-molecule products. The subject invention provides materials and methods for extracting microbial metabolites at a high level of purity, for example, a purity of at least 80% by weight, and preferably at least 95% by weight or more. Specifically, the subject invention provides materials and methods for isolating or extracting biosurfactants and polyketides at a high level of purity. Preferably, the biosurfactant is a sophorolipid (SLP).
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公开(公告)号：US10316052B2
公开(公告)日：2019-06-11
申请号：US15324555
申请日：2015-07-07
发明人： Huijun Ren , Daochao Li , Xuexiao Ying , Feng Chen , Linghui Zheng , Lingping Wang , Hua Bai
摘要： A fidaxomicin purification method, comprising: fermenting Actinoplanes sp. HS-16-20 to generate fermented liquid; conducting solid/liquid separation on the fermented liquid, soaking mycelium in an organic solvent, and filtering to obtain a solution containing fidaxomicin; treating the solution with nanofiltration concentrate, and separating to obtain fidaxomicin crude product; conducting preparative column chromatography on the fidaxomicin crude product, eluting with an acid aqueous solution containing an organic solvent, and separating to obtain the refined fidaxomicin product.
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公开(公告)号：US10119158B2
公开(公告)日：2018-11-06
申请号：US14785696
申请日：2014-04-21
发明人： Ankur Sudhir Vaid , Mitesh Jagdishkumar Patel , Dhimant Punjalal Mehta , Anand Mahadev Dhiman , Jayesh Chandubhai Patel , Traunkant Parshuram Sharma
IPC分类号： C12P19/58 , C07D407/14 , C12P19/62 , C07H17/08 , C12N1/20
摘要： The present invention provides an improved process for the preparation of fidaxomicin by culturing Actinoplanes deccanensis in the culture medium of the invention wherein the fidaxomicin is produced in a yield of greater than 500 mg/L broth. The present invention also provides a whole broth extraction process for the isolation of fidaxomicin from the fermentation broth. The present invention also provides fidaxomicin having purity of greater than 97% area by HPLC.
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公开(公告)号：US20180179244A1
公开(公告)日：2018-06-28
申请号：US15324555
申请日：2015-07-07
发明人： Huijun REN , Daochao LI , Xuexiao YING , Feng CHEN , Linghui ZHENG , Lingping WANG , Hua BAI
摘要： A fidaxomicin purification method, comprising: fermenting Actinoplanes sp. HS-16-20 to generate fermented liquid; conducting solid/liquid separation on the fermented liquid, soaking mycelium in an organic solvent, and filtering to obtain a solution containing fidaxomicin; treating the solution with nanofiltration concentrate, and separating to obtain fidaxomicin crude product; conducting preparative column chromatography on the fidaxomicin crude product, eluting with an acid aqueous solution containing an organic solvent, and separating to obtain the refined fidaxomicin product.
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公开(公告)号：US09970041B2
公开(公告)日：2018-05-15
申请号：US14419955
申请日：2013-07-30
申请人： OLON SPA
发明人： Guido Trione , Antonella Malcangi
摘要： The present invention relates to a procedure for the production of tiacumicin B comprising fermentation of a micro-organism capable of producing tiacumicin B, in particular of the species Dactylosporangium aurantiacum or Actinoplanes deccanensis, in a culture broth containing emulsifiers, such as ethoxylated castor oil, in combination with antifoaming products and vegetable oils.
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公开(公告)号：US09944967B2
公开(公告)日：2018-04-17
申请号：US14913601
申请日：2014-08-20
IPC分类号： C12P19/62 , C07D309/32 , C12N9/10 , C12P7/62
CPC分类号： C12P19/62 , C07D309/32 , C12N9/1025 , C12P7/62
摘要： Organohalides (e.g., organofluorides) represent a rapidly expanding proportion of molecules used in pharmaceuticals, diagnostics, agrochemicals, and materials. The present invention exploits the naturally occurring acetate condensation pathway as a means of introducing halogenated building blocks into natural product scaffolds. In an exemplary embodiment, the invention provides a pathway involving at least one polyketide synthase system and utilizes haloacetate or a haloacetate synthon (e.g., halomalonate) to incorporate an organohalide into the polyketide backbone in vitro. In an exemplary embodiment, the invention provides an analogous method for site-selective introduction of haloacetate or a synthon into polyketide products in vivo. The present invention expands the scope of existing chemical methods for producing halogenated natural product scaffolds such as complex halogenated natural products.
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公开(公告)号：US09670518B2
公开(公告)日：2017-06-06
申请号：US13467783
申请日：2012-05-09
申请人： Adam Meadows
发明人： Adam Meadows
CPC分类号： C12P19/62 , C12N1/38 , C12N15/52 , C12P5/007 , C12P7/6409 , C12P7/6427 , C12P13/04 , C12P17/06
摘要： The present disclosure relates to the use of pantothenate compounds as a non-genetic switch for the production of heterologous acetyl-CoA derived (HACD) compounds in microbial host cells. The invention provides genetically modified microorganisms that are more stable when stored and initially cultured under reduced pantothenate concentrations, cell culture media having reduced concentrations of pantothenate compounds, and methods of producing HACD compounds using the cell culture media and the genetically engineered microorganisms of the invention.
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公开(公告)号：US20170101659A1
公开(公告)日：2017-04-13
申请号：US14903190
申请日：2014-07-09
IPC分类号： C12P21/02 , C12N15/10 , C12P17/08 , C12P17/06 , C12N9/10 , C12P19/62 , C12P17/16 , C12P17/10 , C12P17/12 , C12P19/60 , C12P17/18
CPC分类号： C12P21/02 , A01N43/40 , A01N43/90 , A61K31/436 , A61K31/453 , A61K45/06 , C07D405/06 , C07D491/052 , C07D498/18 , C12N9/1029 , C12N15/1082 , C12P17/06 , C12P17/08 , C12P17/10 , C12P17/12 , C12P17/16 , C12P17/162 , C12P17/181 , C12P17/182 , C12P17/188 , C12P17/189 , C12P19/60 , C12P19/62 , C12Y203/01
摘要： A method for increasing the molecular diversity of polyketides and non-ribsomomal peptides by using recombination to efficiently increase or decrease the number of modules in the polyketide synthase or non-ribosomal peptide synthetase encoding said polyketide or peptide.
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公开(公告)号：US09137992B2
公开(公告)日：2015-09-22
申请号：US14082494
申请日：2013-11-18
申请人： DOW AGROSCIENCES LLC
发明人： Lesley S. Burns , Paul Richard Graupner , Paul Lewer , Christine J. Martin , William A. Vousden , Clive Waldron , Barnie Wilkinson
摘要： The invention provides, biologically active spinosyns, hybrid spinosyn polyketide synthases capable of functioning in Saccharopolyspora spinosa to produce the spinosyns, and methods of controlling insects using the spinosyns.
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公开(公告)号：US09040259B2
公开(公告)日：2015-05-26
申请号：US13046304
申请日：2011-03-11
IPC分类号： C12P19/62 , C12P17/18 , C07D471/18 , C07D487/18 , C07D493/18 , C07K14/36 , C12N15/52 , C12N15/70 , C12N15/76 , C12N1/20
CPC分类号： C07D498/18 , C07D405/12 , C07D471/18 , C07D487/18 , C07D493/18 , C07K14/36 , C12N15/52 , C12N15/70 , C12N15/76 , C12P17/188
摘要： The present invention relates to production of polyketides and other natural products and to libraries of compounds and individual novel compounds. One important area is the isolation and potential use of novel FKBP-ligand analogs and host cells that produce these compounds. The invention is particularly concerned with methods for the efficient transformation of strains that produce FKBP analogs and recombinant cells in which cloned genes or gene cassettes are expressed to generate novel compounds such as polyketide (especially rapamycin) FKBP-ligand analogs, and to processes for their preparation, and to means employed therein (e.g. nucleic acids, vectors, gene cassettes and genetically modified strains).
摘要翻译： 本发明涉及聚酮化合物和其它天然产物的生产以及化合物和单个新化合物的文库。 一个重要的领域是产生这些化合物的新型FKBP-配体类似物和宿主细胞的分离和潜在用途。 本发明特别涉及用于产生FKBP类似物和重组细胞的菌株的有效转化的方法，其中克隆的基因或基因盒被表达以产生新的化合物，例如聚酮化合物（特别是雷帕霉素）FKBP-配体类似物， 制备和其中使用的手段（例如核酸，载体，基因盒和遗传修饰的菌株）。
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