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    Method of removing recalcitrant organic pollutants
    1.
    发明授权
    Method of removing recalcitrant organic pollutants 有权

    公开(公告)号：US09994470B2

    公开(公告)日：2018-06-12

    申请号：US14003667

    申请日：2011-03-07

    申请人： Sijing Wang ,  Zhonghong Cheng ,  Weiqing Xu

    发明人： Sijing Wang ,  Zhonghong Cheng ,  Weiqing Xu

    IPC分类号： C02F3/00 ,  C02F3/10 ,  C02F3/34 ,  C02F3/06 ,  C02F1/28

    CPC分类号： C02F3/348 ,  C02F1/281 ,  C02F1/283 ,  C02F3/06 ,  C02F3/106 ,  C02F3/34 ,  Y02W10/15

    摘要： Recalcitrant chemical oxygen demand (COD) of a liquid is reduced in a water treatment system. The method includes pretreating the liquid in a pretreatment unit to remove indigenous bacteria or microbes to a population level below which the indigenous organisms can interfere with the screened and externally introduced microorganisms. The liquid is then provided to a reactor that has a filter bed formed with a carrier material. Special microbes are screened and used to colonize the carrier material to remove recalcitrant COD. A biofilm is cultured on the surface of the carrier material to immobilize the screened microbes in the reactor. The method further includes percolating the liquid from the pretreatment unit through the filter bed colonized with the screened microbes to degrade at least part of the recalcitrant COD under aerobic conditions. In one embodiment, the filter is formed with biological granular activated carbon (GAC) as the carrier material and the screened microbes comprise at least one microbial species selected from the group consisting of Bacillus, Comamonas, Arthrobacter, Micrococcus, Pseudomonas, Pediococcus, Achromobacter, Flavobacterium, Mycobacterium, Rhodanobacter, Stenotrophomonas and Yeast.

    Process for concentrating and processing fluid samples
    3.
    发明授权
    Process for concentrating and processing fluid samples 有权
    浓缩和处理流体样品的方法

    公开(公告)号：US08158405B2

    公开(公告)日：2012-04-17

    申请号：US12215768

    申请日：2008-06-30

    申请人： Scott Boyette ,  Rong Xu ,  Jing Luo ,  Weiqing Xu ,  Jing Chen ,  Weimin Xiao ,  Tong Chen

    发明人： Scott Boyette ,  Rong Xu ,  Jing Luo ,  Weiqing Xu ,  Jing Chen ,  Weimin Xiao ,  Tong Chen

    IPC分类号： C12N1/06

    CPC分类号： C12Q1/24

    摘要： A method of treating a liquid sample having microbiological target species therein to concentrate the species and collect lysate is disclosed. The liquid sample comprises non-target microbiological particles, inorganic particles, and microbiological target species. The liquid is passed through a prefilter medium to allow the target species to pass through as filtrate and retain non-target microbiological products and inorganic particles thereon. The filtrate is contacted with a main filtration medium adapted to retain the target species thereon as retentate. The retentate is lysed to form a lysate containing target material that was enveloped within the microbiological target species. The microbiological species may comprise cell containing or viral material. Target materials comprise intracellular nucleic acids, or in the case of viral sampling, nucleic acids encased within the protein sheath or coating of the virus.

    摘要翻译： 公开了一种处理其中具有微生物靶物种的液体样品以浓缩物种并收集裂解物的方法。 液体样品包括非目标微生物颗粒，无机颗粒和微生物目标物种。 使液体通过预过滤介质，使目标物质作为滤液通过，并在其上保留非目标微生物产物和无机颗粒。 将滤液与适于将其上保留目标物质的主过滤介质接触，作为保留物。 将渗余物溶解以形成包含在微生物目标物种内的目标物质的裂解物。 微生物物种可以包含含细胞或病毒物质。 目标材料包含细胞内核酸，或者在病毒取样的情况下，包含在病毒的蛋白质鞘或涂层内的核酸。

    Process for concentrating and processing fluid samples
    4.
    发明申请
    Process for concentrating and processing fluid samples 有权
    浓缩和处理流体样品的方法

    公开(公告)号：US20090326211A1

    公开(公告)日：2009-12-31

    申请号：US12215768

    申请日：2008-06-30

    申请人： Scott Boyette ,  Rong Xu ,  Jing Luo ,  Weiqing Xu ,  Jing Chen ,  Weimin Xiao ,  Tong Chen

    发明人： Scott Boyette ,  Rong Xu ,  Jing Luo ,  Weiqing Xu ,  Jing Chen ,  Weimin Xiao ,  Tong Chen

    IPC分类号： C07H1/06 ,  C12N1/06

    CPC分类号： C12Q1/24

    摘要： A method of treating a liquid sample having microbiological target species therein to concentrate the species and collect lysate is disclosed. The liquid sample comprises non-target microbiological particles, inorganic particles, and microbiological target species. The liquid is passed through a prefilter medium to allow the target species to pass through as filtrate and retain non-target microbiological products and inorganic particles thereon. The filtrate is contacted with a main filtration medium adapted to retain the target species thereon as retentate. The retentate is lysed to form a lysate containing target material that was enveloped within the microbiological target species. The microbiological species may comprise cell containing or viral material. Target materials comprise intracellular nucleic acids, or in the case of viral sampling, nucleic acids encased within the protein sheath or coating of the virus.

    摘要翻译： 公开了一种处理其中具有微生物靶物种的液体样品以浓缩物种并收集裂解物的方法。 液体样品包括非目标微生物颗粒，无机颗粒和微生物目标物种。 使液体通过预过滤介质，使目标物质作为滤液通过，并在其上保留非目标微生物产物和无机颗粒。 将滤液与适于将其上保留目标物质的主过滤介质接触，作为保留物。 将渗余物溶解以形成包含在微生物目标物种内的目标物质的裂解物。 微生物物种可以包含含细胞或病毒物质。 目标材料包含细胞内核酸，或者在病毒取样的情况下，包含在病毒的蛋白质鞘或涂层内的核酸。

    METHOD OF REMOVING RECALCITRANT ORGANIC POLLUTANTS
    6.
    发明申请
    METHOD OF REMOVING RECALCITRANT ORGANIC POLLUTANTS 有权
    去除重要有机污染物的方法

    公开(公告)号：US20130334135A1

    公开(公告)日：2013-12-19

    申请号：US14003667

    申请日：2011-03-07

    申请人： Sijing Wang ,  Zhonghong Cheng ,  Weiqing Xu

    发明人： Sijing Wang ,  Zhonghong Cheng ,  Weiqing Xu

    IPC分类号： C02F3/34

    CPC分类号： C02F3/348 ,  C02F1/281 ,  C02F1/283 ,  C02F3/06 ,  C02F3/106 ,  C02F3/34 ,  Y02W10/15

    摘要： Recalcitrant chemical oxygen demand (COD) of a liquid is reduced in a water treatment system. The method includes pretreating the liquid in a pretreatment unit to remove indigenous bacteria or microbes to a population level below which the indigenous organisms can interfere with the screened and externally introduced microorganisms. The liquid is then provided to a reactor that has a filter bed formed with a carrier material. Special microbes are screened and used to colonize the carrier material to remove recalcitrant COD. A biofilm is cultured on the surface of the carrier material to immobilize the screened microbes in the reactor. The method further includes percolating the liquid from the pretreatment unit through the filter bed colonized with the screened microbes to degrade at least part of the recalcitrant COD under aerobic conditions. In one embodiment, the filter is formed with biological granular activated carbon (GAC) as the carrier material and the screened microbes comprise at least one microbial species selected from the group consisting of Bacillus, Comamonas, Arthrobacter, Micrococcus, Pseudomonas, Pediococcus, Achromobacter, Flavobacterium, Mycobacterium, Rhodanobacter, Stenotrophomonas and Yeast.

    摘要翻译： 在水处理系统中，液体的化学需氧量（COD）降低。 该方法包括预处理预处理单元中的液体以将本地细菌或微生物除去至低于该水平的本地生物可能干扰被筛选和外部引入的微生物的群体水平。 然后将液体提供到具有由载体材料形成的过滤床的反应器。 筛选特殊的微生物并用于定植载体材料以除去顽固的COD。 在载体材料的表面上培养生物膜以将筛选的微生物固定在反应器中。 该方法还包括将来自预处理单元的液体渗透通过筛选微生物定殖的滤床，以在需氧条件下降解至少部分顽固性COD。 在一个实施方案中，过滤器由生物颗粒状活性炭（GAC）形成为载体材料，筛选的微生物包含至少一种选自芽孢杆菌属，科马矛菌属，节杆菌属，微球菌属，假单胞菌属，球菌属，无色杆菌属， 黄杆菌属，分枝杆菌属，杜氏杆菌属，嗜麦芽寡养单胞菌属和酵母菌属。

    BIOCHEMICAL PROCESS FOR SELENIUM RECOVERY FROM BIOREMEDIATION EFFLUENT OR SLUDGE
    7.
    发明申请
    BIOCHEMICAL PROCESS FOR SELENIUM RECOVERY FROM BIOREMEDIATION EFFLUENT OR SLUDGE 审中-公开
    从生物降解或污泥中回收硒的生物化学过程

    公开(公告)号：US20130260444A1

    公开(公告)日：2013-10-03

    申请号：US13254993

    申请日：2010-12-17

    申请人： Yan Jin ,  Weiqing Xu ,  Yan Huang

    发明人： Yan Jin ,  Weiqing Xu ,  Yan Huang

    IPC分类号： C01B19/00

    CPC分类号： C01B19/00 ,  C02F3/06 ,  C02F3/34 ,  C02F3/342 ,  C02F2101/10 ,  C02F2101/106 ,  C02F2101/20 ,  C02F2303/06 ,  C02F2305/04 ,  Y02W10/15

    摘要： Wastewater containing selenium in a soluble form is treated in a bioreactor. Microorganisms in the reactor reduce the selenium to elemental selenium, which is insoluble. The elemental selenium is discharged from the reactor in waste sludge. The waste sludge is thickened and then treated with a cell lysis reagent to break down or dissolve micro-organism cells in the sludge. After lysis, the sludge is treated to physically separate out the remaining solids, which includes elemental selenium, for reuse.

    摘要翻译： 在生物反应器中处理含有可溶形式的硒的废水。 反应器中的微生物将硒还原成不溶性的元素硒。 元素硒在废渣中从反应器中排出。 废渣被增稠，然后用细胞裂解试剂处理以分解或溶解污泥中的微生物细胞。 在裂解后，处理污泥以物理分离出剩余的固体，其中包括元素硒，以便重新使用。

    METHOD FOR EVALUATING THE VIRULENCE OF PATHOGENIC BIPHASIC BACTERIA
    8.
    发明申请
    METHOD FOR EVALUATING THE VIRULENCE OF PATHOGENIC BIPHASIC BACTERIA 审中-公开
    评估病原性嗜酸细菌病毒的方法

    公开(公告)号：US20110129843A1

    公开(公告)日：2011-06-02

    申请号：US13054988

    申请日：2009-07-29

    申请人： Scott Martell Boyette ,  Jing Chen ,  Jie Li ,  Weiqing Xu ,  Kechao Yang

    发明人： Scott Martell Boyette ,  Jing Chen ,  Jie Li ,  Weiqing Xu ,  Kechao Yang

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  Y02A50/451 ,  C12Q2561/113

    摘要： A method for evaluating relative bacterial virulence of a biphasic bacteria in environmental systems includes measuring the concentration of DNA in the bacteria, measuring the concentration of RNA in the bacteria, determining a ratio of the concentration of RNA to the concentration of DNA and correlating the concentration ratio with a level of relative pathogenicity, wherein the bacteria is preferentially Legionella pneumophila, Mycobacterium tuberculosis and Listeria.

    摘要翻译： 用于评估环境系统中双相细菌的相对细菌毒力的方法包括测量细菌中DNA的浓度，测量细菌中RNA的浓度，确定RNA的浓度与DNA浓度的比例，并将浓度 比例与相对致病性水平，其中细菌优选为嗜肺军团菌，结核分枝杆菌和李斯特菌。