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1.发明授权Microbiological process for the preparation of a protein by culturing a mutant bacterial strain 失效通过培养突变型菌株制备蛋白质的微生物过程
公开(公告)号：US4945047A
公开(公告)日：1990-07-31
申请号：US36263
申请日：1987-04-09
IPC分类号： C12N15/09 , C07H21/04 , C07K14/61 , C12N1/20 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/67 , C12P21/00 , C12P21/02 , C12R1/19
摘要： The invention relates to a microbiological process for the preparation of a protein by culturing a mutant bacterial strain.This process consists in using a bacterial strain carrying a mutation which limits, or even suppresses, the expression of the operons whose transcription depends on the fixation of the cAMP-CRP complex, a DNA sequence coding for a protein precursor having been introduced into the said bacterial strain.
摘要翻译： 本发明涉及通过培养突变型细菌菌株制备蛋白质的微生物过程。 该方法包括使用携带突变的细菌菌株，其限制或甚至抑制其转录取决于cAMP-CRP复合物的固定的操纵子的表达，编码已引入所述蛋白前体的蛋白质前体的DNA序列 细菌菌株。
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公开(公告)号：US5417970A
公开(公告)日：1995-05-23
申请号：US152886
申请日：1993-11-16
申请人： Willem Roskam, deceased , Bertrand Basuyaux , Pascual Ferrara , Martine Laporte , Thierry Maureaud , Natalio Vita , Alain Bayol , Genevieve Perry
发明人： Willem Roskam, deceased , Bertrand Basuyaux , Pascual Ferrara , Martine Laporte , Thierry Maureaud , Natalio Vita , Alain Bayol , Genevieve Perry
摘要： An interleukin-2 preparation suitable for pharmaceutical purposes, consisting essentially of disialilated glycosylated interleukin-2, monosialilated glycosylated interleukin-2, or a mixture thereof, and substantially free of organic solvents, is isolated from recombinant CHO cells transformed with a vector containing a DNA sequence coding for a natural precursor of human interleukin-2, and is purified by a multi-step process.
摘要翻译： 适用于药物目的的白细胞介素2制剂基本上由重组CHO细胞分离，其由含有DNA的载体转化的重组CHO细胞分离，所述白细胞介素-2制剂基本上由二次唾液酸化的糖基化白细胞介素-2，单唾液酸化的糖基化白细胞介素-2或其混合物和基本上不含有机溶剂组成 编码人白细胞介素-2天然前体的序列，并通过多步法纯化。
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