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公开(公告)号：US10398149B2
公开(公告)日：2019-09-03
申请号：US14428284
申请日：2013-09-10
发明人： Yan Zheng , Ruihua Guo , Tiankui Yang , Xuebing Xu
摘要： A flour product whitener containing polysaccharide alcohol. When the flour product whitener is used in a flour product, the amount of the polysaccharide alcohol is 0.01-10 parts by mass in every 100 parts by mass of total flour in the flour product.
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2.发明申请公开(公告)号：US20160270415A1
公开(公告)日：2016-09-22
申请号：US15032010
申请日：2014-10-24
CPC分类号： A23D9/013 , A23D9/007 , A23D9/04 , A23L33/17 , A23V2002/00 , C11B5/0028 , C11B5/005 , C11B5/0071
摘要： A composition including algal oil body(ies), a modified algal oil body, a method for stabilizing an algal oil body, and a method for improving the oxidation resistance of an algal oil body and/or preventing oil leakage of an algal oil body are disclosed. Specifically, a modified algal oil body including an algal of body, a phospholipid and an of body protein, a method for improving the stability of an algal oil body by adding an oil body protein and a phospholipid into the algal oil body, and a method for improving oxidation resistance of an algal oil body by adding thereinto an oil body protein are disclosed.
摘要翻译： 包含藻油体，改性藻油体，稳定藻油体的方法的组合物，以及提高藻油体的抗氧化性和/或防止藻油体的油渗漏的方法， 披露 具体地说，包括人体，磷脂和身体蛋白质的藻类的改性藻体，通过向海藻油体中添加油体蛋白质和磷脂来提高藻油体的稳定性的方法， 公开了通过添加油体蛋白来改善藻油体的抗氧化性的方法。
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公开(公告)号：US20220073889A1
公开(公告)日：2022-03-10
申请号：US17419082
申请日：2019-12-27
发明人： Yaoji XUAN , Qiwen NIU , Zhengjun XU
摘要： Provided is a polypeptide having phospholipase C activity. The polypeptide has: 1) an amino acid sequence represented by SEQ ID No: 2, comprising amino acid substitutions occurred at one or more positions, wherein the one or more positions are selected from positions 6, 8, 10, 104, and 205 in the amino acid sequence represented by SEQ ID No: 2 or any combination thereof; or 2) having at least 80% sequence identity with 1), and at least one of positions 6, 8, 10, 104 and 205 being different from positions 6, 8, 10, 104, and 205 in the amino acid sequence represented by SEQ ID No: 2. Provided are a nucleic acid molecule for encoding the polypeptide, a vector comprising the nucleic acid molecule, and a cell comprising the nucleic acid molecule or the vector. Provided are uses of the polypeptide, the nucleic acid molecule, the vector, and the cell.
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公开(公告)号：US09969995B2
公开(公告)日：2018-05-15
申请号：US15529657
申请日：2015-11-27
CPC分类号： C12N9/2445 , C07K2319/00 , C07K2319/02 , C12N9/58 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y304/23 , C12Y304/23023
摘要： Provided is a method for recombinant expression of β-glucosidase gene. Also provided is a recombinant expression vector comprising: (a) a coding sequence of an aspartic protease or active fragment thereof, (b) a coding sequence of β-glucosidase or active fragment thereof, and optionally (c) a linker sequence between (a) and (b). Further provided are the recombinant host cell and the recombinant cellulose-degrading microorganism comprising the recombinant expression vector, the preparation method and uses thereof.
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公开(公告)号：US20220162543A1
公开(公告)日：2022-05-26
申请号：US17419122
申请日：2019-12-27
发明人： Wei Wu , Xiaojun Dai , Haisheng Cao , Meifeng Zhou , Qiwen Niu
摘要： Provided is a Pichia pastoris mutant strain for expressing an exogenous gene. Specifically, provided is a Pichia pastoris mutant strain comprising, with respect to Pichia pastoris mutant strain GS115 or CICC32806, one or more of the following six mutations: BQ9382_C1-2260, EKK deletions at positions 308-310, a hypothetical protein; BQ9382_C1-3800, E129K, 60S ribosomal subunit assembly/exported protein LOC1; BQ9382_C1-5700, I312M, mitochondrial external NADH dehydrogenase, type II NAD(P)H:quinone oxidoreductase; BQ9382_C2-3950, Q145X, an essential protein having a binding partner Psr1p and used for completely activating a general stress response; BQ9382_C3-2220, E188K, a hypothetical protein; and BQ9382_C3-4370, W196X, orotidine 5\′-phosphate decarboxylase. The provided Pichia pastoris mutant strain is an effective commonly employed host for exogenous expression, and can efficiently express different proteins, especially phospholipase and lipase.
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公开(公告)号：US11618865B2
公开(公告)日：2023-04-04
申请号：US16957618
申请日：2018-12-24
发明人： Xiang Chen , Yan Zheng , Yongqing Chi
IPC分类号： C11C3/06 , A23L33/115 , A23G1/36 , C11C3/10
摘要： The present disclosure relates to an oil and fat composition, use thereof and a food comprising the oil and fat composition. The oil and fat composition of the disclosure comprises 50-99.5 wt % triglyceride and 0.5-50 wt % wax by the total weight of the composition, the triglyceride is represented by the following formula (1), and the definitions of the groups in formula (1) are as recorded in the description. The oil and fat composition is suitable for preparation of a food.
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公开(公告)号：US20200037654A1
公开(公告)日：2020-02-06
申请号：US16491824
申请日：2018-05-03
申请人： Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences , Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd.
发明人： Qiang WANG , Li LIU , Jinchuang ZHANG , Song ZHU , Aimin SHI , Hui HU , Hongzhi LIU , Yuanrong JIANG , Yuquan ZHANG
IPC分类号： A23L33/185 , A23L19/10 , A23L29/00 , A23P30/10 , A23P30/25
摘要： A peanut protein vegetarian sausage and a preparation method thereof are described. The peanut protein vegetarian sausage is prepared from components including low-temperature defatted peanut protein powder, soybean protein isolate, vital wheat gluten, and modifier. The vegetarian sausage has a protein content of 60% or more, zero cholesterol and low fat, and has the characteristics of good taste, being rich in nutrients and convenience to eat. Also described are a preparation method of the vegetarian sausage, which includes mixing the main materials, performing an extrusion treatment, adding water and an auxiliary material online during the extrusion, and cooling the product obtained after extrusion molding to give the peanut protein vegetarian sausage. The preparation method is a pure physical processing method with a high degree of automation, and the processing is free from any pollutant discharge.
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公开(公告)号：US20180362942A1
公开(公告)日：2018-12-20
申请号：US16061574
申请日：2016-12-15
摘要： Provided is a mutant of the wild type phosphatidylcholine-specific phospholipase C of Bacillus cereus. The mutations involved comprise the amino acid residue at position 63 being mutated from asparagine to aspartic acid, the amino acid residue at position 131 being mutated from asparagine to serine, and the amino acid residue at position 134 being mutated from asparagine to aspartic acid, and may comprise the amino acid residue at position 56 being mutated from tyrosine to alanine, lysine, asparagine, glutamine, histidine or tryptophan, and further, may also comprise the amino acid residue at position 106 being mutated from methionine to valine. Also provided are a polynucleotide molecule encoding the mutant, a nucleic acid construct and a host cell comprising the polynucleotide molecule, a composition comprising the mutant, and the use of the mutant, the polynucleotide molecule, the nucleic acid construct and the host cell.
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公开(公告)号：US10144919B2
公开(公告)日：2018-12-04
申请号：US15035105
申请日：2014-11-04
发明人： Jun Xu , Yaoji Xuan , Jinmin Li
摘要： The present application provides mutants of wild type phospholipase C (PLC) specific to phosphatidylcholine from Bacillus cereus. The related mutations include mutation of asparagine at position 63 to another amino acid, also including mutation of arginine at position 20 to histidine and of alanine at position 83 to aspartic acid. The present application also provides a nucleic acid molecule encoding the mutant, a vector containing the nucleic acid molecule, and a cell containing the nucleic acid molecule or vector. The present application also provides uses of the mutant, nucleic acid molecule vector, and cell.
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公开(公告)号：US20170327807A1
公开(公告)日：2017-11-16
申请号：US15529657
申请日：2015-11-27
CPC分类号： C12N9/2445 , C07K2319/00 , C07K2319/02 , C12N9/58 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y304/23 , C12Y304/23023
摘要： Provided is a method for recombinant expression of β-glucosidase gene. Also provided is a recombinant expression vector comprising: (a) a coding sequence of an aspartic protease or active fragment thereof, (b) a coding sequence of β-glucosidase or active fragment thereof, and optionally (c) a linker sequence between (a) and (b). Further provided are the recombinant host cell and the recombinant cellulose-degrading microorganism comprising the recombinant expression vector, the preparation method and uses thereof.
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