Separation method and device for protein mixtures
    1.
    发明申请
    Separation method and device for protein mixtures 失效
    蛋白质混合物的分离方法和装置

    公开(公告)号:US20060000712A1

    公开(公告)日:2006-01-05

    申请号:US11120098

    申请日:2005-05-02

    IPC分类号: B01D57/02 C02F1/40

    CPC分类号: G01N27/44773

    摘要: A separation device and a separation method for biomolecular sample material and in particular protein mixtures. For this purpose a separation element 10 for the two-dimensional and preferable electrophoretic separation of components of the sample material is provided in area 30 of a separation plane. According to the invention it is proposed that the separation element 10 has a channel or transfer structure 14 for the locally resolved discharge of separated sample components in a transport direction that is at right angles to the separation plane onto a support surface 16 that is preferably suitable for mass spectroscopic analyses.

    摘要翻译: 用于生物分子样品材料,特别是蛋白质混合物的分离装置和分离方法。 为此,在分离平面的区域30中提供用于样品材料的组分的二维和优选的电泳分离的分离元件10。 根据本发明,提出了分离元件10具有通道或转移结构14,用于将分离的样品组分在与分离平面成直角的运输方向上局部分解排放到支撑表面16上,其优选地适合 用于质谱分析。

    Matrix reactor and a method for producing products in said reactor
    3.
    发明授权
    Matrix reactor and a method for producing products in said reactor 失效
    基质反应器和在所述反应器中产生产物的方法

    公开(公告)号:US07192767B2

    公开(公告)日:2007-03-20

    申请号:US10221124

    申请日:2001-03-07

    摘要: The present invention concerns a matrix reactor for reactions in which low-molecular and high-molecular reactants are involved wherein the reactor space VR contains a matrix and comprises a reaction compartment and a supply compartment, wherein the matrix is composed of a porous material which can take up low-molecular reactants and wherein the exclusion volume V0 of the matrix is available as the reaction compartment and the matrix volume VM of the matrix is available as the supply compartment. The matrix reactor can be used for enzymatic processes, in particular for a coupled in vitro transcription/translation reaction to produce proteins.

    摘要翻译: 本发明涉及用于其中涉及低分子和高分子反应物的反应的基质反应器,其中反应器空间V R R含有基质并且包含反应室和供应室,其中基质 由可以吸收低分子量反应物的多孔材料组成,并且其中基质的排阻体积V 0 O可用作反应室,并且基体体积V M 的矩阵可用作供应隔室。 基质反应器可用于酶法,特别是用于偶联的体外转录/翻译反应以产生蛋白质。

    Separation method and device for protein mixtures
    5.
    发明授权
    Separation method and device for protein mixtures 失效
    蛋白质混合物的分离方法和装置

    公开(公告)号:US07645369B2

    公开(公告)日:2010-01-12

    申请号:US11120098

    申请日:2005-05-02

    IPC分类号: G01N27/447 G01N27/453

    CPC分类号: G01N27/44773

    摘要: A separation device and a separation method for biomolecular sample material and in particular protein mixtures. For this purpose a separation element 10 for the two-dimensional and preferable electrophoretic separation of components of the sample material is provided in area 30 of a separation plane. According to the invention it is proposed that the separation element 10 has a channel or transfer structure 14 for the locally resolved discharge of separated sample components in a transport direction that is at right angles to the separation plane onto a support surface 16 that is preferably suitable for mass spectroscopic analyses.

    摘要翻译: 用于生物分子样品材料,特别是蛋白质混合物的分离装置和分离方法。 为此,在分离平面的区域30中提供用于样品材料的组分的二维和优选的电泳分离的分离元件10。 根据本发明,提出了分离元件10具有通道或转移结构14,用于将分离的样品组分在与分离平面成直角的运输方向上局部分解排放到支撑表面16上,其优选地适合 用于质谱分析。