摘要:
A separation device and a separation method for biomolecular sample material and in particular protein mixtures. For this purpose a separation element 10 for the two-dimensional and preferable electrophoretic separation of components of the sample material is provided in area 30 of a separation plane. According to the invention it is proposed that the separation element 10 has a channel or transfer structure 14 for the locally resolved discharge of separated sample components in a transport direction that is at right angles to the separation plane onto a support surface 16 that is preferably suitable for mass spectroscopic analyses.
摘要:
Diagnostic methods for in vivo and ex vivo detection of circulating tumor cells (CTCs) for the diagnosis and treatment of cancer are provided. The diagnostic methods employ oncolytic viruses alone or in combination with one or more tumor cell enrichment and/or detection methods. Combinations and kits for use in the practicing the methods also are provided.
摘要:
The present invention concerns a matrix reactor for reactions in which low-molecular and high-molecular reactants are involved wherein the reactor space VR contains a matrix and comprises a reaction compartment and a supply compartment, wherein the matrix is composed of a porous material which can take up low-molecular reactants and wherein the exclusion volume V0 of the matrix is available as the reaction compartment and the matrix volume VM of the matrix is available as the supply compartment. The matrix reactor can be used for enzymatic processes, in particular for a coupled in vitro transcription/translation reaction to produce proteins.
摘要翻译:本发明涉及用于其中涉及低分子和高分子反应物的反应的基质反应器,其中反应器空间V R R含有基质并且包含反应室和供应室,其中基质 由可以吸收低分子量反应物的多孔材料组成,并且其中基质的排阻体积V 0 O可用作反应室,并且基体体积V M 的矩阵可用作供应隔室。 基质反应器可用于酶法,特别是用于偶联的体外转录/翻译反应以产生蛋白质。
摘要:
A separation device and a separation method for biomolecular sample material and in particular protein mixtures. For this purpose a separation element 10 for the two-dimensional and preferable electrophoretic separation of components of the sample material is provided in area 30 of a separation plane. According to the invention it is proposed that the separation element 10 has a channel or transfer structure 14 for the locally resolved discharge of separated sample components in a transport direction that is at right angles to the separation plane onto a support surface 16 that is preferably suitable for mass spectroscopic analyses.